Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2587 and Psest_2588 are separated by 136 nucleotides Psest_2588 and Psest_2589 are separated by 54 nucleotides
Psest_2587: Psest_2587 - Aspartate-semialdehyde dehydrogenase, at 2,768,900 to 2,769,910
_2587
Psest_2588: Psest_2588 - aspartate-semialdehyde dehydrogenase, gamma-proteobacterial, at 2,770,047 to 2,771,159
_2588
Psest_2589: Psest_2589 - 3-isopropylmalate dehydrogenase, at 2,771,214 to 2,772,296
_2589
Position (kb)
2770
2771
2772 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2769.170 kb on + strand, within Psest_2587 at 2769.170 kb on + strand, within Psest_2587 at 2769.170 kb on + strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.171 kb on - strand, within Psest_2587 at 2769.378 kb on - strand, within Psest_2587 at 2769.378 kb on - strand, within Psest_2587 at 2769.378 kb on - strand, within Psest_2587 at 2769.449 kb on - strand, within Psest_2587 at 2769.462 kb on - strand, within Psest_2587 at 2769.462 kb on - strand, within Psest_2587 at 2769.743 kb on + strand, within Psest_2587 at 2769.863 kb on + strand at 2769.864 kb on - strand at 2769.904 kb on + strand at 2769.904 kb on + strand at 2769.905 kb on - strand at 2769.992 kb on + strand at 2769.992 kb on + strand at 2769.993 kb on - strand at 2769.993 kb on - strand at 2769.993 kb on - strand at 2771.167 kb on - strand at 2771.167 kb on - strand at 2771.167 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.173 kb on - strand at 2771.175 kb on - strand at 2771.175 kb on - strand at 2771.175 kb on - strand at 2771.467 kb on + strand, within Psest_2589 at 2771.468 kb on - strand, within Psest_2589 at 2771.468 kb on - strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.632 kb on + strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.633 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.663 kb on - strand, within Psest_2589 at 2771.822 kb on - strand, within Psest_2589 at 2771.875 kb on + strand, within Psest_2589 at 2771.876 kb on - strand, within Psest_2589 at 2771.876 kb on - strand, within Psest_2589 at 2772.146 kb on - strand, within Psest_2589
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,769,170 + Psest_2587 0.27 +1.3 2,769,170 + Psest_2587 0.27 +1.0 2,769,170 + Psest_2587 0.27 -0.3 2,769,171 - Psest_2587 0.27 -1.0 2,769,171 - Psest_2587 0.27 -0.4 2,769,171 - Psest_2587 0.27 -0.4 2,769,171 - Psest_2587 0.27 -0.2 2,769,171 - Psest_2587 0.27 -0.1 2,769,171 - Psest_2587 0.27 +1.9 2,769,171 - Psest_2587 0.27 -0.1 2,769,171 - Psest_2587 0.27 +0.8 2,769,378 - Psest_2587 0.47 -0.2 2,769,378 - Psest_2587 0.47 +0.2 2,769,378 - Psest_2587 0.47 +0.0 2,769,449 - Psest_2587 0.54 +1.0 2,769,462 - Psest_2587 0.56 -0.2 2,769,462 - Psest_2587 0.56 -0.2 2,769,743 + Psest_2587 0.83 +0.0 2,769,863 + +1.0 2,769,864 - -0.3 2,769,904 + +0.8 2,769,904 + +0.3 2,769,905 - -0.0 2,769,992 + +0.5 2,769,992 + -0.2 2,769,993 - +0.4 2,769,993 - -0.7 2,769,993 - -0.3 2,771,167 - -0.5 2,771,167 - -0.4 2,771,167 - -1.0 2,771,173 - -1.6 2,771,173 - -0.7 2,771,173 - +0.3 2,771,173 - -1.5 2,771,173 - -1.9 2,771,173 - -1.2 2,771,173 - +0.3 2,771,175 - -0.8 2,771,175 - +0.6 2,771,175 - -0.3 2,771,467 + Psest_2589 0.23 -3.8 2,771,468 - Psest_2589 0.23 -2.5 2,771,468 - Psest_2589 0.23 -2.9 2,771,632 + Psest_2589 0.39 -1.0 2,771,632 + Psest_2589 0.39 -0.5 2,771,632 + Psest_2589 0.39 -2.0 2,771,632 + Psest_2589 0.39 -4.2 2,771,632 + Psest_2589 0.39 -2.7 2,771,633 - Psest_2589 0.39 -4.1 2,771,633 - Psest_2589 0.39 -2.9 2,771,633 - Psest_2589 0.39 -1.3 2,771,633 - Psest_2589 0.39 -5.1 2,771,633 - Psest_2589 0.39 -4.3 2,771,633 - Psest_2589 0.39 +0.0 2,771,633 - Psest_2589 0.39 -0.5 2,771,633 - Psest_2589 0.39 -2.7 2,771,633 - Psest_2589 0.39 -2.0 2,771,633 - Psest_2589 0.39 -5.0 2,771,633 - Psest_2589 0.39 -1.5 2,771,633 - Psest_2589 0.39 -2.4 2,771,633 - Psest_2589 0.39 -1.1 2,771,633 - Psest_2589 0.39 -2.4 2,771,633 - Psest_2589 0.39 -2.8 2,771,633 - Psest_2589 0.39 -3.3 2,771,633 - Psest_2589 0.39 -3.3 2,771,633 - Psest_2589 0.39 -1.8 2,771,633 - Psest_2589 0.39 -0.1 2,771,633 - Psest_2589 0.39 -3.2 2,771,633 - Psest_2589 0.39 -2.1 2,771,633 - Psest_2589 0.39 -4.8 2,771,633 - Psest_2589 0.39 -2.7 2,771,633 - Psest_2589 0.39 -3.8 2,771,633 - Psest_2589 0.39 -4.2 2,771,633 - Psest_2589 0.39 -3.1 2,771,633 - Psest_2589 0.39 -2.8 2,771,633 - Psest_2589 0.39 -3.7 2,771,633 - Psest_2589 0.39 -3.2 2,771,633 - Psest_2589 0.39 -1.5 2,771,663 - Psest_2589 0.41 -3.0 2,771,663 - Psest_2589 0.41 -2.5 2,771,663 - Psest_2589 0.41 -0.8 2,771,663 - Psest_2589 0.41 -2.5 2,771,663 - Psest_2589 0.41 -4.0 2,771,822 - Psest_2589 0.56 -3.1 2,771,875 + Psest_2589 0.61 -2.3 2,771,876 - Psest_2589 0.61 -0.8 2,771,876 - Psest_2589 0.61 -3.4 2,772,146 - Psest_2589 0.86 -0.9
Or see this region's nucleotide sequence