Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2218 and Psest_2219 overlap by 4 nucleotides Psest_2219 and Psest_2220 overlap by 1 nucleotides
Psest_2218: Psest_2218 - Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit, at 2,367,168 to 2,368,142
_2218
Psest_2219: Psest_2219 - pyruvate dehydrogenase E1 component, alpha subunit, at 2,368,139 to 2,369,131
_2219
Psest_2220: Psest_2220 - Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases, at 2,369,131 to 2,370,888
_2220
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2368.161 kb on + strand at 2368.161 kb on + strand at 2368.161 kb on + strand at 2368.162 kb on - strand at 2368.162 kb on - strand at 2368.308 kb on + strand, within Psest_2219 at 2368.308 kb on + strand, within Psest_2219 at 2368.308 kb on + strand, within Psest_2219 at 2368.309 kb on - strand, within Psest_2219 at 2368.362 kb on + strand, within Psest_2219 at 2368.402 kb on - strand, within Psest_2219 at 2368.402 kb on - strand, within Psest_2219 at 2368.422 kb on + strand, within Psest_2219 at 2368.857 kb on + strand, within Psest_2219 at 2368.858 kb on - strand, within Psest_2219 at 2368.858 kb on - strand, within Psest_2219 at 2368.920 kb on + strand, within Psest_2219 at 2368.968 kb on - strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.992 kb on + strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2368.993 kb on - strand, within Psest_2219 at 2369.505 kb on - strand, within Psest_2220 at 2369.547 kb on - strand, within Psest_2220 at 2369.547 kb on - strand, within Psest_2220 at 2369.594 kb on + strand, within Psest_2220 at 2369.595 kb on - strand, within Psest_2220 at 2369.656 kb on + strand, within Psest_2220 at 2369.961 kb on - strand, within Psest_2220 at 2370.071 kb on + strand, within Psest_2220 at 2370.071 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.113 kb on + strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.114 kb on - strand, within Psest_2220 at 2370.158 kb on + strand, within Psest_2220 at 2370.158 kb on + strand, within Psest_2220 at 2370.158 kb on + strand, within Psest_2220 at 2370.159 kb on - strand, within Psest_2220 at 2370.159 kb on - strand, within Psest_2220 at 2370.159 kb on - strand, within Psest_2220 at 2370.159 kb on - strand, within Psest_2220 at 2370.347 kb on + strand, within Psest_2220 at 2370.502 kb on + strand, within Psest_2220 at 2370.539 kb on + strand, within Psest_2220 at 2370.540 kb on - strand, within Psest_2220 at 2370.540 kb on - strand, within Psest_2220 at 2370.643 kb on + strand, within Psest_2220 at 2370.643 kb on + strand, within Psest_2220 at 2370.643 kb on + strand, within Psest_2220 at 2370.643 kb on + strand, within Psest_2220 at 2370.643 kb on + strand, within Psest_2220 at 2370.644 kb on - strand, within Psest_2220 at 2370.644 kb on - strand, within Psest_2220 at 2370.647 kb on + strand, within Psest_2220 at 2370.648 kb on - strand, within Psest_2220 at 2370.882 kb on + strand at 2370.888 kb on + strand at 2371.054 kb on + strand at 2371.055 kb on - strand at 2371.183 kb on - strand at 2371.257 kb on + strand at 2371.257 kb on + strand at 2371.258 kb on - strand at 2371.281 kb on + strand at 2371.291 kb on + strand at 2371.291 kb on + strand at 2371.292 kb on - strand at 2371.790 kb on + strand at 2371.790 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,368,161 + +0.3 2,368,161 + +0.7 2,368,161 + -1.4 2,368,162 - -0.5 2,368,162 - +0.7 2,368,308 + Psest_2219 0.17 +0.2 2,368,308 + Psest_2219 0.17 -0.6 2,368,308 + Psest_2219 0.17 -0.6 2,368,309 - Psest_2219 0.17 -0.2 2,368,362 + Psest_2219 0.22 -1.0 2,368,402 - Psest_2219 0.26 -1.3 2,368,402 - Psest_2219 0.26 -0.9 2,368,422 + Psest_2219 0.28 +1.3 2,368,857 + Psest_2219 0.72 +1.0 2,368,858 - Psest_2219 0.72 -0.1 2,368,858 - Psest_2219 0.72 -0.6 2,368,920 + Psest_2219 0.79 -0.2 2,368,968 - Psest_2219 0.83 +0.1 2,368,992 + Psest_2219 0.86 -0.0 2,368,992 + Psest_2219 0.86 +1.1 2,368,992 + Psest_2219 0.86 +1.1 2,368,992 + Psest_2219 0.86 -0.4 2,368,992 + Psest_2219 0.86 +2.0 2,368,992 + Psest_2219 0.86 -1.2 2,368,992 + Psest_2219 0.86 +0.5 2,368,993 - Psest_2219 0.86 +1.0 2,368,993 - Psest_2219 0.86 -0.0 2,368,993 - Psest_2219 0.86 -1.3 2,368,993 - Psest_2219 0.86 +1.0 2,368,993 - Psest_2219 0.86 +0.7 2,368,993 - Psest_2219 0.86 +1.0 2,368,993 - Psest_2219 0.86 +1.1 2,369,505 - Psest_2220 0.21 -0.3 2,369,547 - Psest_2220 0.24 -0.5 2,369,547 - Psest_2220 0.24 +1.0 2,369,594 + Psest_2220 0.26 -0.0 2,369,595 - Psest_2220 0.26 -1.0 2,369,656 + Psest_2220 0.30 +1.7 2,369,961 - Psest_2220 0.47 -0.6 2,370,071 + Psest_2220 0.53 +0.3 2,370,071 + Psest_2220 0.53 -0.0 2,370,113 + Psest_2220 0.56 -0.1 2,370,113 + Psest_2220 0.56 -0.3 2,370,113 + Psest_2220 0.56 +0.2 2,370,113 + Psest_2220 0.56 -1.5 2,370,113 + Psest_2220 0.56 -0.5 2,370,113 + Psest_2220 0.56 -2.3 2,370,113 + Psest_2220 0.56 +0.8 2,370,113 + Psest_2220 0.56 +0.1 2,370,113 + Psest_2220 0.56 +0.4 2,370,113 + Psest_2220 0.56 -2.2 2,370,114 - Psest_2220 0.56 +0.5 2,370,114 - Psest_2220 0.56 +0.7 2,370,114 - Psest_2220 0.56 -1.8 2,370,114 - Psest_2220 0.56 -0.7 2,370,114 - Psest_2220 0.56 -1.6 2,370,114 - Psest_2220 0.56 +0.6 2,370,114 - Psest_2220 0.56 -0.3 2,370,158 + Psest_2220 0.58 -1.8 2,370,158 + Psest_2220 0.58 -0.4 2,370,158 + Psest_2220 0.58 -0.9 2,370,159 - Psest_2220 0.58 +1.9 2,370,159 - Psest_2220 0.58 -0.1 2,370,159 - Psest_2220 0.58 +1.6 2,370,159 - Psest_2220 0.58 +1.7 2,370,347 + Psest_2220 0.69 +1.8 2,370,502 + Psest_2220 0.78 +0.6 2,370,539 + Psest_2220 0.80 -0.7 2,370,540 - Psest_2220 0.80 -0.9 2,370,540 - Psest_2220 0.80 +0.3 2,370,643 + Psest_2220 0.86 -0.6 2,370,643 + Psest_2220 0.86 -0.1 2,370,643 + Psest_2220 0.86 -0.2 2,370,643 + Psest_2220 0.86 +0.9 2,370,643 + Psest_2220 0.86 +3.0 2,370,644 - Psest_2220 0.86 -0.4 2,370,644 - Psest_2220 0.86 +1.6 2,370,647 + Psest_2220 0.86 +1.5 2,370,648 - Psest_2220 0.86 +1.3 2,370,882 + -0.2 2,370,888 + +0.9 2,371,054 + -0.0 2,371,055 - -0.2 2,371,183 - -1.0 2,371,257 + -0.2 2,371,257 + -0.0 2,371,258 - +0.4 2,371,281 + +1.4 2,371,291 + -0.8 2,371,291 + -0.2 2,371,292 - +0.5 2,371,790 + -1.3 2,371,790 + -0.2
Or see this region's nucleotide sequence