Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1462 and Psest_1463 are separated by 3 nucleotides Psest_1463 and Psest_1464 overlap by 4 nucleotides Psest_1464 and Psest_1465 are separated by 75 nucleotides
Psest_1462: Psest_1462 - 3-oxoacyl-[acyl-carrier-protein] synthase III, at 1,570,051 to 1,571,172
_1462
Psest_1463: Psest_1463 - SufE protein probably involved in Fe-S center assembly, at 1,571,176 to 1,571,598
_1463
Psest_1464: Psest_1464 - Selenocysteine lyase, at 1,571,595 to 1,572,800
_1464
Psest_1465: Psest_1465 - 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase, at 1,572,876 to 1,573,910
_1465
Position (kb)
1571
1572
1573 Strain fitness (log2 ratio)
-2
-1
0
1 at 1570.666 kb on - strand, within Psest_1462 at 1570.666 kb on - strand, within Psest_1462 at 1571.036 kb on - strand, within Psest_1462 at 1571.036 kb on - strand, within Psest_1462 at 1571.036 kb on - strand, within Psest_1462 at 1571.036 kb on - strand, within Psest_1462 at 1571.036 kb on - strand, within Psest_1462 at 1571.212 kb on - strand at 1571.469 kb on + strand, within Psest_1463 at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.662 kb on + strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.663 kb on - strand at 1571.664 kb on + strand at 1571.665 kb on - strand at 1571.935 kb on + strand, within Psest_1464 at 1572.028 kb on + strand, within Psest_1464 at 1572.029 kb on - strand, within Psest_1464 at 1572.081 kb on + strand, within Psest_1464 at 1572.100 kb on + strand, within Psest_1464 at 1572.101 kb on - strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.151 kb on + strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.152 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.253 kb on - strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.667 kb on + strand, within Psest_1464 at 1572.668 kb on - strand, within Psest_1464 at 1572.668 kb on - strand, within Psest_1464 at 1572.670 kb on + strand, within Psest_1464 at 1572.730 kb on + strand at 1572.730 kb on + strand at 1572.882 kb on - strand at 1572.882 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,570,666 - Psest_1462 0.55 +0.2 1,570,666 - Psest_1462 0.55 -0.9 1,571,036 - Psest_1462 0.88 -1.4 1,571,036 - Psest_1462 0.88 -1.1 1,571,036 - Psest_1462 0.88 -1.7 1,571,036 - Psest_1462 0.88 -1.5 1,571,036 - Psest_1462 0.88 -0.0 1,571,212 - -0.2 1,571,469 + Psest_1463 0.69 -0.1 1,571,662 + +0.6 1,571,662 + -0.2 1,571,662 + -0.5 1,571,662 + -0.2 1,571,662 + +0.1 1,571,662 + -0.4 1,571,662 + +0.7 1,571,662 + +0.1 1,571,662 + -1.1 1,571,662 + +0.2 1,571,662 + -2.7 1,571,662 + -0.7 1,571,662 + +0.7 1,571,662 + -0.9 1,571,663 - +0.5 1,571,663 - +0.3 1,571,663 - -2.4 1,571,663 - -1.1 1,571,663 - -1.3 1,571,663 - +0.7 1,571,663 - +0.1 1,571,663 - -1.2 1,571,663 - -0.3 1,571,663 - +0.1 1,571,663 - +0.4 1,571,663 - +0.4 1,571,664 + +0.5 1,571,665 - -0.8 1,571,935 + Psest_1464 0.28 -0.2 1,572,028 + Psest_1464 0.36 -0.6 1,572,029 - Psest_1464 0.36 -1.7 1,572,081 + Psest_1464 0.40 -0.1 1,572,100 + Psest_1464 0.42 +0.1 1,572,101 - Psest_1464 0.42 -0.8 1,572,151 + Psest_1464 0.46 -0.5 1,572,151 + Psest_1464 0.46 -0.6 1,572,151 + Psest_1464 0.46 -0.6 1,572,151 + Psest_1464 0.46 -0.5 1,572,152 - Psest_1464 0.46 +0.6 1,572,152 - Psest_1464 0.46 +0.6 1,572,152 - Psest_1464 0.46 -0.7 1,572,152 - Psest_1464 0.46 +0.0 1,572,152 - Psest_1464 0.46 -0.4 1,572,152 - Psest_1464 0.46 -0.4 1,572,253 - Psest_1464 0.55 -0.4 1,572,253 - Psest_1464 0.55 +0.6 1,572,253 - Psest_1464 0.55 -0.5 1,572,667 + Psest_1464 0.89 -0.1 1,572,667 + Psest_1464 0.89 -0.1 1,572,667 + Psest_1464 0.89 -1.5 1,572,667 + Psest_1464 0.89 -0.2 1,572,667 + Psest_1464 0.89 -0.4 1,572,668 - Psest_1464 0.89 -0.5 1,572,668 - Psest_1464 0.89 +1.1 1,572,670 + Psest_1464 0.89 -0.6 1,572,730 + -0.2 1,572,730 + -1.1 1,572,882 - +0.0 1,572,882 - -0.3
Or see this region's nucleotide sequence