Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1266 and Psest_1267 are separated by 4 nucleotides Psest_1267 and Psest_1268 are separated by 158 nucleotides Psest_1268 and Psest_1269 overlap by 13 nucleotides Psest_1269 and Psest_1270 are separated by 11 nucleotides
Psest_1266: Psest_1266 - ribosome-associated GTPase EngA, at 1,371,182 to 1,372,675
_1266
Psest_1267: Psest_1267 - serine O-acetyltransferase, at 1,372,680 to 1,373,474
_1267
Psest_1268: Psest_1268 - Aspartate/tyrosine/aromatic aminotransferase, at 1,373,633 to 1,374,781
_1268
Psest_1269: Psest_1269 - Predicted amidohydrolase, at 1,374,769 to 1,375,563
_1269
Psest_1270: Psest_1270 - hypothetical protein, at 1,375,575 to 1,375,766
_1270
Position (kb)
1373
1374
1375 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.965 kb on + strand, within Psest_1267 at 1372.966 kb on - strand, within Psest_1267 at 1372.966 kb on - strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.166 kb on + strand, within Psest_1267 at 1373.167 kb on - strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.265 kb on + strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.266 kb on - strand, within Psest_1267 at 1373.486 kb on + strand at 1373.486 kb on + strand at 1373.487 kb on - strand at 1373.487 kb on - strand at 1373.487 kb on - strand at 1373.523 kb on + strand at 1373.524 kb on - strand at 1373.644 kb on + strand at 1373.645 kb on - strand at 1373.677 kb on + strand at 1373.758 kb on - strand, within Psest_1268 at 1373.973 kb on + strand, within Psest_1268 at 1373.996 kb on + strand, within Psest_1268 at 1374.015 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.206 kb on + strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.207 kb on - strand, within Psest_1268 at 1374.228 kb on - strand, within Psest_1268 at 1374.287 kb on + strand, within Psest_1268 at 1374.287 kb on + strand, within Psest_1268 at 1374.353 kb on + strand, within Psest_1268 at 1374.404 kb on + strand, within Psest_1268 at 1374.498 kb on - strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.572 kb on + strand, within Psest_1268 at 1374.768 kb on + strand at 1374.976 kb on + strand, within Psest_1269 at 1374.976 kb on + strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1374.977 kb on - strand, within Psest_1269 at 1375.016 kb on + strand, within Psest_1269 at 1375.017 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.106 kb on - strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.150 kb on + strand, within Psest_1269 at 1375.151 kb on - strand, within Psest_1269 at 1375.274 kb on - strand, within Psest_1269 at 1375.351 kb on + strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.352 kb on - strand, within Psest_1269 at 1375.567 kb on + strand at 1375.568 kb on - strand at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.612 kb on + strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.613 kb on - strand, within Psest_1270 at 1375.640 kb on - strand, within Psest_1270 at 1375.760 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,372,965 + Psest_1267 0.36 +0.2 1,372,965 + Psest_1267 0.36 -0.4 1,372,965 + Psest_1267 0.36 +0.6 1,372,965 + Psest_1267 0.36 -0.3 1,372,965 + Psest_1267 0.36 -0.1 1,372,965 + Psest_1267 0.36 +0.6 1,372,966 - Psest_1267 0.36 -0.1 1,372,966 - Psest_1267 0.36 +0.7 1,373,166 + Psest_1267 0.61 +0.8 1,373,166 + Psest_1267 0.61 +1.6 1,373,166 + Psest_1267 0.61 -0.2 1,373,167 - Psest_1267 0.61 +2.0 1,373,265 + Psest_1267 0.74 +1.3 1,373,265 + Psest_1267 0.74 -0.2 1,373,265 + Psest_1267 0.74 -0.2 1,373,265 + Psest_1267 0.74 -0.6 1,373,265 + Psest_1267 0.74 -0.3 1,373,266 - Psest_1267 0.74 +0.1 1,373,266 - Psest_1267 0.74 +0.2 1,373,266 - Psest_1267 0.74 -0.0 1,373,486 + -0.0 1,373,486 + +1.3 1,373,487 - -0.8 1,373,487 - +0.7 1,373,487 - -0.4 1,373,523 + -0.7 1,373,524 - -0.2 1,373,644 + -0.3 1,373,645 - +1.0 1,373,677 + -0.2 1,373,758 - Psest_1268 0.11 +0.6 1,373,973 + Psest_1268 0.30 -0.2 1,373,996 + Psest_1268 0.32 -0.2 1,374,015 + Psest_1268 0.33 +0.0 1,374,206 + Psest_1268 0.50 -0.5 1,374,206 + Psest_1268 0.50 -1.0 1,374,206 + Psest_1268 0.50 +0.8 1,374,206 + Psest_1268 0.50 -0.6 1,374,207 - Psest_1268 0.50 -0.8 1,374,207 - Psest_1268 0.50 -0.9 1,374,207 - Psest_1268 0.50 -0.1 1,374,207 - Psest_1268 0.50 +0.4 1,374,207 - Psest_1268 0.50 +0.0 1,374,207 - Psest_1268 0.50 +1.4 1,374,207 - Psest_1268 0.50 +0.6 1,374,228 - Psest_1268 0.52 -0.4 1,374,287 + Psest_1268 0.57 -0.7 1,374,287 + Psest_1268 0.57 -0.7 1,374,353 + Psest_1268 0.63 +0.6 1,374,404 + Psest_1268 0.67 -0.7 1,374,498 - Psest_1268 0.75 -1.7 1,374,572 + Psest_1268 0.82 +0.5 1,374,572 + Psest_1268 0.82 +0.9 1,374,572 + Psest_1268 0.82 +0.1 1,374,572 + Psest_1268 0.82 +0.6 1,374,768 + -0.8 1,374,976 + Psest_1269 0.26 -0.3 1,374,976 + Psest_1269 0.26 -0.9 1,374,977 - Psest_1269 0.26 +0.6 1,374,977 - Psest_1269 0.26 -0.8 1,374,977 - Psest_1269 0.26 -0.1 1,375,016 + Psest_1269 0.31 -0.4 1,375,017 - Psest_1269 0.31 -0.3 1,375,106 - Psest_1269 0.42 -0.6 1,375,106 - Psest_1269 0.42 -0.3 1,375,106 - Psest_1269 0.42 +0.2 1,375,150 + Psest_1269 0.48 -1.9 1,375,150 + Psest_1269 0.48 +0.8 1,375,151 - Psest_1269 0.48 -0.7 1,375,274 - Psest_1269 0.64 -0.2 1,375,351 + Psest_1269 0.73 +0.4 1,375,352 - Psest_1269 0.73 -0.4 1,375,352 - Psest_1269 0.73 +0.2 1,375,567 + +0.5 1,375,568 - +0.2 1,375,612 + Psest_1270 0.19 -0.0 1,375,612 + Psest_1270 0.19 -0.0 1,375,612 + Psest_1270 0.19 +0.2 1,375,612 + Psest_1270 0.19 +0.9 1,375,613 - Psest_1270 0.20 -0.1 1,375,613 - Psest_1270 0.20 +0.1 1,375,640 - Psest_1270 0.34 +0.9 1,375,760 + +1.1
Or see this region's nucleotide sequence