Experiment: m.b. Vancomycin 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00560 and PGA1_262p00570 are separated by 27 nucleotides PGA1_262p00570 and PGA1_262p00580 are separated by 26 nucleotides PGA1_262p00580 and PGA1_262p00590 are separated by 34 nucleotides PGA1_262p00590 and PGA1_262p00600 overlap by 4 nucleotides
PGA1_262p00560: PGA1_262p00560 - uracil DNA glycosylase-like protein, at 65,033 to 66,478
_262p00560
PGA1_262p00570: PGA1_262p00570 - hypothetical protein, at 66,506 to 66,763
_262p00570
PGA1_262p00580: PGA1_262p00580 - putative polysaccharide biosynthesis/export protein, at 66,790 to 68,163
_262p00580
PGA1_262p00590: PGA1_262p00590 - Protein of unknown function (DUF2886)., at 68,198 to 68,857
_262p00590
PGA1_262p00600: PGA1_262p00600 - Bacterial putative lipoprotein (DUF940)., at 68,854 to 70,983
_262p00600
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 66.019 kb on - strand, within PGA1_262p00560 at 66.035 kb on - strand, within PGA1_262p00560 at 66.311 kb on + strand, within PGA1_262p00560 at 66.365 kb on - strand at 66.532 kb on + strand, within PGA1_262p00570 at 66.540 kb on - strand, within PGA1_262p00570 at 66.540 kb on - strand, within PGA1_262p00570 at 66.542 kb on + strand, within PGA1_262p00570 at 66.542 kb on + strand, within PGA1_262p00570 at 66.661 kb on + strand, within PGA1_262p00570 at 66.692 kb on - strand, within PGA1_262p00570 at 66.751 kb on + strand at 66.754 kb on - strand at 66.761 kb on + strand at 66.844 kb on - strand at 66.924 kb on + strand at 66.932 kb on - strand, within PGA1_262p00580 at 66.932 kb on - strand, within PGA1_262p00580 at 67.007 kb on + strand, within PGA1_262p00580 at 67.007 kb on + strand, within PGA1_262p00580 at 67.055 kb on + strand, within PGA1_262p00580 at 67.063 kb on - strand, within PGA1_262p00580 at 67.135 kb on + strand, within PGA1_262p00580 at 67.137 kb on + strand, within PGA1_262p00580 at 67.197 kb on + strand, within PGA1_262p00580 at 67.205 kb on - strand, within PGA1_262p00580 at 67.234 kb on + strand, within PGA1_262p00580 at 67.240 kb on - strand, within PGA1_262p00580 at 67.368 kb on - strand, within PGA1_262p00580 at 67.368 kb on - strand, within PGA1_262p00580 at 67.738 kb on - strand, within PGA1_262p00580 at 67.875 kb on - strand, within PGA1_262p00580 at 67.875 kb on - strand, within PGA1_262p00580 at 67.875 kb on - strand, within PGA1_262p00580 at 68.008 kb on - strand, within PGA1_262p00580 at 68.008 kb on - strand, within PGA1_262p00580 at 68.101 kb on - strand at 68.197 kb on + strand at 68.208 kb on + strand at 68.322 kb on - strand, within PGA1_262p00590 at 68.423 kb on - strand, within PGA1_262p00590 at 68.643 kb on - strand, within PGA1_262p00590 at 68.709 kb on - strand, within PGA1_262p00590 at 68.742 kb on - strand, within PGA1_262p00590 at 68.771 kb on + strand, within PGA1_262p00590 at 68.798 kb on - strand at 68.850 kb on + strand at 68.892 kb on + strand at 68.956 kb on + strand at 68.982 kb on - strand at 69.035 kb on + strand at 69.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Vancomycin 0.032 mg/ml remove 66,019 - PGA1_262p00560 0.68 +0.4 66,035 - PGA1_262p00560 0.69 -0.1 66,311 + PGA1_262p00560 0.88 +1.1 66,365 - +3.7 66,532 + PGA1_262p00570 0.10 +0.1 66,540 - PGA1_262p00570 0.13 -1.1 66,540 - PGA1_262p00570 0.13 -2.1 66,542 + PGA1_262p00570 0.14 -1.0 66,542 + PGA1_262p00570 0.14 +0.5 66,661 + PGA1_262p00570 0.60 -1.3 66,692 - PGA1_262p00570 0.72 +0.4 66,751 + +2.6 66,754 - +1.4 66,761 + -0.7 66,844 - -0.8 66,924 + -2.7 66,932 - PGA1_262p00580 0.10 -0.9 66,932 - PGA1_262p00580 0.10 -1.6 67,007 + PGA1_262p00580 0.16 -1.6 67,007 + PGA1_262p00580 0.16 -0.1 67,055 + PGA1_262p00580 0.19 -1.4 67,063 - PGA1_262p00580 0.20 +0.1 67,135 + PGA1_262p00580 0.25 -0.6 67,137 + PGA1_262p00580 0.25 -2.8 67,197 + PGA1_262p00580 0.30 -1.0 67,205 - PGA1_262p00580 0.30 -0.6 67,234 + PGA1_262p00580 0.32 -1.6 67,240 - PGA1_262p00580 0.33 -1.2 67,368 - PGA1_262p00580 0.42 -1.4 67,368 - PGA1_262p00580 0.42 -2.2 67,738 - PGA1_262p00580 0.69 -3.7 67,875 - PGA1_262p00580 0.79 +0.8 67,875 - PGA1_262p00580 0.79 -1.0 67,875 - PGA1_262p00580 0.79 -1.2 68,008 - PGA1_262p00580 0.89 -1.1 68,008 - PGA1_262p00580 0.89 -1.1 68,101 - -0.1 68,197 + +0.8 68,208 + -0.2 68,322 - PGA1_262p00590 0.19 -1.0 68,423 - PGA1_262p00590 0.34 +0.8 68,643 - PGA1_262p00590 0.67 -1.2 68,709 - PGA1_262p00590 0.77 +1.4 68,742 - PGA1_262p00590 0.82 -0.7 68,771 + PGA1_262p00590 0.87 -2.2 68,798 - -0.7 68,850 + +0.8 68,892 + -0.2 68,956 + -0.8 68,982 - -2.3 69,035 + -0.4 69,043 - +0.5
Or see this region's nucleotide sequence