Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0412 and Psest_0413 overlap by 8 nucleotides Psest_0413 and Psest_0414 overlap by 8 nucleotides Psest_0414 and Psest_0415 are separated by 1 nucleotides
Psest_0412: Psest_0412 - 8-amino-7-oxononanoate synthase, at 447,762 to 448,940
_0412
Psest_0413: Psest_0413 - Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily), at 448,933 to 449,658
_0413
Psest_0414: Psest_0414 - biotin biosynthesis protein BioC, at 449,651 to 450,445
_0414
Psest_0415: Psest_0415 - dethiobiotin synthase, at 450,447 to 451,133
_0415
Position (kb) 448
449
450 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.080 kb on + strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.081 kb on - strand, within Psest_0412 at 448.082 kb on + strand, within Psest_0412 at 448.083 kb on - strand, within Psest_0412 at 448.282 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.545 kb on + strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.546 kb on - strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.551 kb on + strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.552 kb on - strand, within Psest_0412 at 448.613 kb on + strand, within Psest_0412 at 448.614 kb on - strand, within Psest_0412 at 448.859 kb on + strand at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.196 kb on + strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.197 kb on - strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.341 kb on + strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.342 kb on - strand, within Psest_0413 at 449.461 kb on + strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.462 kb on - strand, within Psest_0413 at 449.721 kb on - strand at 449.721 kb on - strand at 449.721 kb on - strand at 449.993 kb on + strand, within Psest_0414 at 449.993 kb on + strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 449.994 kb on - strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.182 kb on + strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.183 kb on - strand, within Psest_0414 at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.407 kb on + strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.408 kb on - strand at 450.420 kb on - strand at 450.420 kb on - strand at 450.557 kb on - strand, within Psest_0415
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 448,080 + Psest_0412 0.27 -2.9 448,080 + Psest_0412 0.27 -4.0 448,080 + Psest_0412 0.27 -1.6 448,080 + Psest_0412 0.27 -2.0 448,080 + Psest_0412 0.27 -0.6 448,080 + Psest_0412 0.27 -2.7 448,080 + Psest_0412 0.27 -0.6 448,081 - Psest_0412 0.27 -1.7 448,081 - Psest_0412 0.27 -2.3 448,081 - Psest_0412 0.27 -2.9 448,081 - Psest_0412 0.27 -3.3 448,082 + Psest_0412 0.27 -2.6 448,083 - Psest_0412 0.27 -1.3 448,282 + Psest_0412 0.44 +1.3 448,545 + Psest_0412 0.66 -2.9 448,545 + Psest_0412 0.66 -1.1 448,545 + Psest_0412 0.66 -2.4 448,545 + Psest_0412 0.66 -2.6 448,546 - Psest_0412 0.66 -2.2 448,546 - Psest_0412 0.66 -5.1 448,546 - Psest_0412 0.66 -2.5 448,551 + Psest_0412 0.67 -2.9 448,551 + Psest_0412 0.67 -2.9 448,551 + Psest_0412 0.67 -3.4 448,552 - Psest_0412 0.67 -3.0 448,552 - Psest_0412 0.67 -2.1 448,552 - Psest_0412 0.67 -1.7 448,552 - Psest_0412 0.67 -1.5 448,552 - Psest_0412 0.67 -1.9 448,552 - Psest_0412 0.67 -2.2 448,552 - Psest_0412 0.67 -1.1 448,552 - Psest_0412 0.67 -1.1 448,552 - Psest_0412 0.67 -1.5 448,552 - Psest_0412 0.67 -1.3 448,552 - Psest_0412 0.67 -2.3 448,552 - Psest_0412 0.67 -2.5 448,552 - Psest_0412 0.67 -2.6 448,552 - Psest_0412 0.67 -3.9 448,552 - Psest_0412 0.67 -2.9 448,613 + Psest_0412 0.72 -2.8 448,614 - Psest_0412 0.72 -1.3 448,859 + -2.7 449,196 + Psest_0413 0.36 +0.3 449,196 + Psest_0413 0.36 -4.4 449,196 + Psest_0413 0.36 -1.3 449,196 + Psest_0413 0.36 -1.9 449,197 - Psest_0413 0.36 -0.8 449,197 - Psest_0413 0.36 -2.0 449,197 - Psest_0413 0.36 -3.1 449,197 - Psest_0413 0.36 -2.5 449,197 - Psest_0413 0.36 -3.1 449,341 + Psest_0413 0.56 -1.8 449,341 + Psest_0413 0.56 -1.1 449,341 + Psest_0413 0.56 -1.2 449,342 - Psest_0413 0.56 -1.3 449,342 - Psest_0413 0.56 -1.8 449,461 + Psest_0413 0.73 -1.9 449,462 - Psest_0413 0.73 -0.7 449,462 - Psest_0413 0.73 -3.0 449,721 - -2.8 449,721 - -3.0 449,721 - -2.7 449,993 + Psest_0414 0.43 -1.2 449,993 + Psest_0414 0.43 -2.3 449,994 - Psest_0414 0.43 -2.4 449,994 - Psest_0414 0.43 -3.1 449,994 - Psest_0414 0.43 -2.7 450,182 + Psest_0414 0.67 -2.0 450,182 + Psest_0414 0.67 -1.9 450,182 + Psest_0414 0.67 -3.1 450,182 + Psest_0414 0.67 -1.5 450,183 - Psest_0414 0.67 -2.1 450,183 - Psest_0414 0.67 -1.1 450,183 - Psest_0414 0.67 -4.1 450,183 - Psest_0414 0.67 -1.5 450,407 + -2.4 450,407 + -2.1 450,407 + -2.3 450,407 + -3.1 450,408 - -2.5 450,408 - -0.3 450,408 - -3.2 450,408 - -0.5 450,420 - -2.5 450,420 - -1.1 450,557 - Psest_0415 0.16 -2.3
Or see this region's nucleotide sequence