Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_2021

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_2019 and Psest_2020 are separated by 87 nucleotidesPsest_2020 and Psest_2021 overlap by 4 nucleotidesPsest_2021 and Psest_2022 are separated by 69 nucleotidesPsest_2022 and Psest_2023 are separated by 102 nucleotides Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434 _2019 Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160 _2020 Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284 _2021 Psest_2022: Psest_2022 - ADP-ribose pyrophosphatase, at 2,141,354 to 2,141,794 _2022 Psest_2023: Psest_2023 - isocitrate dehydrogenase, NADP-dependent, monomeric type, at 2,141,897 to 2,144,125 _2023 Position (kb) 2140 2141 2142Strain fitness (log2 ratio) -2 -1 0 1 2at 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.483 kb on - strandat 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.754 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.756 kb on + strand, within Psest_2020at 2139.757 kb on - strand, within Psest_2020at 2139.905 kb on - strand, within Psest_2020at 2139.910 kb on - strand, within Psest_2020at 2140.000 kb on + strand, within Psest_2020at 2141.285 kb on - strandat 2141.553 kb on + strand, within Psest_2022at 2141.553 kb on + strand, within Psest_2022at 2141.580 kb on + strand, within Psest_2022at 2141.581 kb on - strand, within Psest_2022at 2141.586 kb on + strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.587 kb on - strand, within Psest_2022at 2141.777 kb on - strandat 2141.794 kb on + strandat 2141.795 kb on - strandat 2141.810 kb on + strandat 2141.810 kb on + strandat 2141.810 kb on + strandat 2141.811 kb on - strandat 2141.811 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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2,139,483 - -0.4
2,139,483 - +0.3
2,139,483 - +0.7
2,139,754 + Psest_2020 0.36 +0.6
2,139,754 + Psest_2020 0.36 -1.4
2,139,754 + Psest_2020 0.36 -1.9
2,139,756 + Psest_2020 0.37 -1.6
2,139,756 + Psest_2020 0.37 -0.6
2,139,757 - Psest_2020 0.37 -1.2
2,139,905 - Psest_2020 0.60 -0.3
2,139,910 - Psest_2020 0.61 +0.2
2,140,000 + Psest_2020 0.75 +0.3
2,141,285 - +0.2
2,141,553 + Psest_2022 0.45 +0.9
2,141,553 + Psest_2022 0.45 +0.3
2,141,580 + Psest_2022 0.51 -1.1
2,141,581 - Psest_2022 0.51 +0.7
2,141,586 + Psest_2022 0.53 -0.4
2,141,587 - Psest_2022 0.53 +1.6
2,141,587 - Psest_2022 0.53 -2.2
2,141,587 - Psest_2022 0.53 +1.8
2,141,777 - -0.9
2,141,794 + +0.6
2,141,795 - -0.4
2,141,810 + +0.8
2,141,810 + -0.0
2,141,810 + +0.6
2,141,811 - +0.2
2,141,811 - +0.7

Or see this region's nucleotide sequence