Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2019 and Psest_2020 are separated by 87 nucleotides Psest_2020 and Psest_2021 overlap by 4 nucleotides Psest_2021 and Psest_2022 are separated by 69 nucleotides Psest_2022 and Psest_2023 are separated by 102 nucleotides
Psest_2019: Psest_2019 - adenylosuccinate lyase, at 2,138,064 to 2,139,434
_2019
Psest_2020: Psest_2020 - Uncharacterized protein involved in purine metabolism, at 2,139,522 to 2,140,160
_2020
Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284
_2021
Psest_2022: Psest_2022 - ADP-ribose pyrophosphatase, at 2,141,354 to 2,141,794
_2022
Psest_2023: Psest_2023 - isocitrate dehydrogenase, NADP-dependent, monomeric type, at 2,141,897 to 2,144,125
_2023
Position (kb)
2140
2141
2142 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.483 kb on - strand at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.754 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.756 kb on + strand, within Psest_2020 at 2139.757 kb on - strand, within Psest_2020 at 2139.905 kb on - strand, within Psest_2020 at 2139.910 kb on - strand, within Psest_2020 at 2140.000 kb on + strand, within Psest_2020 at 2141.285 kb on - strand at 2141.553 kb on + strand, within Psest_2022 at 2141.553 kb on + strand, within Psest_2022 at 2141.580 kb on + strand, within Psest_2022 at 2141.581 kb on - strand, within Psest_2022 at 2141.586 kb on + strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.777 kb on - strand at 2141.794 kb on + strand at 2141.795 kb on - strand at 2141.810 kb on + strand at 2141.810 kb on + strand at 2141.810 kb on + strand at 2141.811 kb on - strand at 2141.811 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 2,139,483 - -0.4 2,139,483 - +0.3 2,139,483 - +0.7 2,139,754 + Psest_2020 0.36 +0.6 2,139,754 + Psest_2020 0.36 -1.4 2,139,754 + Psest_2020 0.36 -1.9 2,139,756 + Psest_2020 0.37 -1.6 2,139,756 + Psest_2020 0.37 -0.6 2,139,757 - Psest_2020 0.37 -1.2 2,139,905 - Psest_2020 0.60 -0.3 2,139,910 - Psest_2020 0.61 +0.2 2,140,000 + Psest_2020 0.75 +0.3 2,141,285 - +0.2 2,141,553 + Psest_2022 0.45 +0.9 2,141,553 + Psest_2022 0.45 +0.3 2,141,580 + Psest_2022 0.51 -1.1 2,141,581 - Psest_2022 0.51 +0.7 2,141,586 + Psest_2022 0.53 -0.4 2,141,587 - Psest_2022 0.53 +1.6 2,141,587 - Psest_2022 0.53 -2.2 2,141,587 - Psest_2022 0.53 +1.8 2,141,777 - -0.9 2,141,794 + +0.6 2,141,795 - -0.4 2,141,810 + +0.8 2,141,810 + -0.0 2,141,810 + +0.6 2,141,811 - +0.2 2,141,811 - +0.7
Or see this region's nucleotide sequence