Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1251

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1249 and Psest_1250 are separated by 159 nucleotidesPsest_1250 and Psest_1251 are separated by 31 nucleotidesPsest_1251 and Psest_1252 are separated by 43 nucleotidesPsest_1252 and Psest_1253 are separated by 11 nucleotidesPsest_1253 and Psest_1254 are separated by 7 nucleotides Psest_1249: Psest_1249 - serine O-acetyltransferase, at 1,356,862 to 1,357,638 _1249 Psest_1250: Psest_1250 - iron-sulfur cluster assembly transcription factor IscR, at 1,357,798 to 1,358,289 _1250 Psest_1251: Psest_1251 - cysteine desulfurase IscS, at 1,358,321 to 1,359,535 _1251 Psest_1252: Psest_1252 - FeS cluster assembly scaffold IscU, at 1,359,579 to 1,359,965 _1252 Psest_1253: Psest_1253 - iron-sulfur cluster assembly protein IscA, at 1,359,977 to 1,360,300 _1253 Psest_1254: Psest_1254 - Fe-S protein assembly co-chaperone HscB, at 1,360,308 to 1,360,829 _1254 Position (kb) 1358 1359 1360Strain fitness (log2 ratio) -1 0 1at 1357.450 kb on - strand, within Psest_1249at 1357.450 kb on - strand, within Psest_1249at 1357.525 kb on - strand, within Psest_1249at 1357.525 kb on - strand, within Psest_1249at 1357.663 kb on + strandat 1357.853 kb on + strand, within Psest_1250at 1357.853 kb on + strand, within Psest_1250at 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strandat 1358.267 kb on + strandat 1359.564 kb on + strandat 1359.564 kb on + strandat 1359.564 kb on + strandat 1360.294 kb on + strandat 1360.294 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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1,357,450 - Psest_1249 0.76 +1.7
1,357,450 - Psest_1249 0.76 +1.3
1,357,525 - Psest_1249 0.85 +1.5
1,357,525 - Psest_1249 0.85 +0.9
1,357,663 + -0.3
1,357,853 + Psest_1250 0.11 -0.8
1,357,853 + Psest_1250 0.11 -0.9
1,358,267 + +0.0
1,358,267 + -0.3
1,358,267 + -0.5
1,358,267 + +0.5
1,358,267 + -0.7
1,359,564 + -0.4
1,359,564 + -0.8
1,359,564 + +0.8
1,360,294 + -0.8
1,360,294 + -0.2

Or see this region's nucleotide sequence