Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1249 and Psest_1250 are separated by 159 nucleotides Psest_1250 and Psest_1251 are separated by 31 nucleotides Psest_1251 and Psest_1252 are separated by 43 nucleotides Psest_1252 and Psest_1253 are separated by 11 nucleotides Psest_1253 and Psest_1254 are separated by 7 nucleotides
Psest_1249: Psest_1249 - serine O-acetyltransferase, at 1,356,862 to 1,357,638
_1249
Psest_1250: Psest_1250 - iron-sulfur cluster assembly transcription factor IscR, at 1,357,798 to 1,358,289
_1250
Psest_1251: Psest_1251 - cysteine desulfurase IscS, at 1,358,321 to 1,359,535
_1251
Psest_1252: Psest_1252 - FeS cluster assembly scaffold IscU, at 1,359,579 to 1,359,965
_1252
Psest_1253: Psest_1253 - iron-sulfur cluster assembly protein IscA, at 1,359,977 to 1,360,300
_1253
Psest_1254: Psest_1254 - Fe-S protein assembly co-chaperone HscB, at 1,360,308 to 1,360,829
_1254
Position (kb)
1358
1359
1360 Strain fitness (log2 ratio)
-1
0
1 at 1357.450 kb on - strand, within Psest_1249 at 1357.450 kb on - strand, within Psest_1249 at 1357.525 kb on - strand, within Psest_1249 at 1357.525 kb on - strand, within Psest_1249 at 1357.663 kb on + strand at 1357.853 kb on + strand, within Psest_1250 at 1357.853 kb on + strand, within Psest_1250 at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand at 1358.267 kb on + strand at 1359.564 kb on + strand at 1359.564 kb on + strand at 1359.564 kb on + strand at 1360.294 kb on + strand at 1360.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 1,357,450 - Psest_1249 0.76 +1.7 1,357,450 - Psest_1249 0.76 +1.3 1,357,525 - Psest_1249 0.85 +1.5 1,357,525 - Psest_1249 0.85 +0.9 1,357,663 + -0.3 1,357,853 + Psest_1250 0.11 -0.8 1,357,853 + Psest_1250 0.11 -0.9 1,358,267 + +0.0 1,358,267 + -0.3 1,358,267 + -0.5 1,358,267 + +0.5 1,358,267 + -0.7 1,359,564 + -0.4 1,359,564 + -0.8 1,359,564 + +0.8 1,360,294 + -0.8 1,360,294 + -0.2
Or see this region's nucleotide sequence