Experiment: D,L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0957 and secG are separated by 1 nucleotides secG and Sama_R0035 are separated by 12 nucleotides Sama_R0035 and Sama_R0036 are separated by 79 nucleotides Sama_R0036 and Sama_0959 are separated by 170 nucleotides Sama_0959 and nusA are separated by 27 nucleotides
Sama_0957: Sama_0957 - Triose-phosphate isomerase (RefSeq), at 1,157,568 to 1,158,350
_0957
Sama_0958: secG - preprotein translocase subunit SecG (RefSeq), at 1,158,352 to 1,158,678
secG
Sama_R0035: Sama_R0035 - tRNA-Leu (RefSeq), at 1,158,691 to 1,158,777
_R0035
Sama_R0036: Sama_R0036 - tRNA-Met (RefSeq), at 1,158,857 to 1,158,933
_R0036
Sama_0959: Sama_0959 - hypothetical protein (RefSeq), at 1,159,104 to 1,159,559
_0959
Sama_0960: nusA - transcription elongation factor NusA (RefSeq), at 1,159,587 to 1,161,086
nusA
Position (kb)
1158
1159 Strain fitness (log2 ratio)
-2
-1
0
1 at 1158.334 kb on + strand at 1158.624 kb on + strand, within secG at 1158.780 kb on + strand at 1158.791 kb on + strand at 1158.799 kb on + strand at 1158.823 kb on + strand at 1158.831 kb on + strand at 1158.831 kb on + strand at 1158.839 kb on + strand at 1158.841 kb on + strand at 1158.841 kb on + strand at 1158.841 kb on + strand at 1158.841 kb on + strand at 1158.971 kb on + strand at 1158.983 kb on + strand at 1158.983 kb on + strand at 1158.983 kb on + strand at 1158.983 kb on + strand at 1158.983 kb on + strand at 1158.983 kb on + strand at 1159.019 kb on + strand at 1159.021 kb on + strand at 1159.021 kb on + strand at 1159.072 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate remove 1,158,334 + -0.5 1,158,624 + secG Sama_0958 0.83 -1.0 1,158,780 + +0.8 1,158,791 + -2.1 1,158,799 + -2.6 1,158,823 + -1.6 1,158,831 + -1.8 1,158,831 + -1.0 1,158,839 + -2.3 1,158,841 + -1.5 1,158,841 + -0.5 1,158,841 + -1.7 1,158,841 + -2.2 1,158,971 + +0.7 1,158,983 + -0.9 1,158,983 + -0.1 1,158,983 + -0.2 1,158,983 + -0.5 1,158,983 + -1.3 1,158,983 + -1.8 1,159,019 + -0.4 1,159,021 + -0.5 1,159,021 + -2.3 1,159,072 + +0.5
Or see this region's nucleotide sequence