Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05590 and PGA1_c05600 are separated by 261 nucleotides PGA1_c05600 and PGA1_c05610 overlap by 1 nucleotides PGA1_c05610 and PGA1_c05620 overlap by 4 nucleotides PGA1_c05620 and PGA1_c05630 are separated by 25 nucleotides
PGA1_c05590: PGA1_c05590 - acyl-CoA dehydrogenase-like protein, at 572,698 to 573,936
_c05590
PGA1_c05600: PGA1_c05600 - queuosine biosynthesis protein QueC, at 574,198 to 574,896
_c05600
PGA1_c05610: PGA1_c05610 - putative queuosine biosynthesis protein QueD, at 574,896 to 575,249
_c05610
PGA1_c05620: PGA1_c05620 - putative 7-cyano-7-deazaguanosine biosynthesis protein QueE, at 575,246 to 576,043
_c05620
PGA1_c05630: PGA1_c05630 - NADPH-dependent 7-cyano-7-deazaguanine reductase QueF, at 576,069 to 576,533
_c05630
Position (kb) 574
575
576 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 573.918 kb on - strand at 573.999 kb on + strand at 573.999 kb on + strand at 573.999 kb on + strand at 574.007 kb on - strand at 574.025 kb on - strand at 574.080 kb on + strand at 574.080 kb on + strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.088 kb on - strand at 574.093 kb on - strand at 574.094 kb on - strand at 574.094 kb on - strand at 574.135 kb on - strand at 574.152 kb on + strand at 574.153 kb on - strand at 574.215 kb on - strand at 574.215 kb on - strand at 574.272 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.295 kb on + strand, within PGA1_c05600 at 574.357 kb on - strand, within PGA1_c05600 at 574.448 kb on + strand, within PGA1_c05600 at 574.451 kb on - strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.467 kb on + strand, within PGA1_c05600 at 574.474 kb on + strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.475 kb on - strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.533 kb on + strand, within PGA1_c05600 at 574.566 kb on - strand, within PGA1_c05600 at 574.582 kb on + strand, within PGA1_c05600 at 574.600 kb on + strand, within PGA1_c05600 at 574.604 kb on + strand, within PGA1_c05600 at 574.657 kb on - strand, within PGA1_c05600 at 574.672 kb on + strand, within PGA1_c05600 at 574.693 kb on + strand, within PGA1_c05600 at 574.760 kb on - strand, within PGA1_c05600 at 574.768 kb on + strand, within PGA1_c05600 at 574.803 kb on + strand, within PGA1_c05600 at 574.852 kb on + strand at 574.988 kb on + strand, within PGA1_c05610 at 575.012 kb on + strand, within PGA1_c05610 at 575.017 kb on + strand, within PGA1_c05610 at 575.022 kb on + strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.030 kb on - strand, within PGA1_c05610 at 575.035 kb on - strand, within PGA1_c05610 at 575.059 kb on + strand, within PGA1_c05610 at 575.106 kb on + strand, within PGA1_c05610 at 575.124 kb on - strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.139 kb on + strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.147 kb on - strand, within PGA1_c05610 at 575.187 kb on + strand, within PGA1_c05610 at 575.237 kb on + strand at 575.322 kb on + strand at 575.377 kb on + strand, within PGA1_c05620 at 575.408 kb on + strand, within PGA1_c05620 at 575.432 kb on + strand, within PGA1_c05620 at 575.433 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.465 kb on - strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.520 kb on + strand, within PGA1_c05620 at 575.525 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.617 kb on + strand, within PGA1_c05620 at 575.651 kb on + strand, within PGA1_c05620 at 575.671 kb on + strand, within PGA1_c05620 at 575.684 kb on - strand, within PGA1_c05620 at 575.702 kb on - strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.761 kb on + strand, within PGA1_c05620 at 575.779 kb on - strand, within PGA1_c05620 at 575.822 kb on + strand, within PGA1_c05620 at 575.884 kb on + strand, within PGA1_c05620 at 575.890 kb on + strand, within PGA1_c05620 at 575.979 kb on + strand at 575.987 kb on - strand at 576.016 kb on + strand at 576.070 kb on + strand at 576.101 kb on + strand at 576.109 kb on + strand at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.117 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.122 kb on - strand, within PGA1_c05630 at 576.138 kb on - strand, within PGA1_c05630 at 576.148 kb on - strand, within PGA1_c05630 at 576.174 kb on + strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.226 kb on - strand, within PGA1_c05630 at 576.239 kb on + strand, within PGA1_c05630
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 573,918 - -1.2 573,999 + +1.3 573,999 + -1.2 573,999 + -0.1 574,007 - +0.8 574,025 - -0.1 574,080 + -0.9 574,080 + -2.7 574,088 - -0.1 574,088 - -0.1 574,088 - -2.4 574,088 - +0.9 574,093 - -0.1 574,094 - -0.4 574,094 - +0.9 574,135 - +0.6 574,152 + +0.9 574,153 - +1.3 574,215 - -0.3 574,215 - +1.1 574,272 + PGA1_c05600 0.11 +1.2 574,295 + PGA1_c05600 0.14 +0.9 574,295 + PGA1_c05600 0.14 -0.2 574,295 + PGA1_c05600 0.14 +1.5 574,357 - PGA1_c05600 0.23 +0.9 574,448 + PGA1_c05600 0.36 -0.9 574,451 - PGA1_c05600 0.36 -2.3 574,467 + PGA1_c05600 0.38 +0.7 574,467 + PGA1_c05600 0.38 +0.9 574,467 + PGA1_c05600 0.38 -0.1 574,474 + PGA1_c05600 0.39 +2.5 574,475 - PGA1_c05600 0.40 -0.1 574,475 - PGA1_c05600 0.40 -1.1 574,475 - PGA1_c05600 0.40 -0.9 574,475 - PGA1_c05600 0.40 +0.9 574,533 + PGA1_c05600 0.48 -0.4 574,533 + PGA1_c05600 0.48 -1.2 574,566 - PGA1_c05600 0.53 -0.9 574,582 + PGA1_c05600 0.55 +1.9 574,600 + PGA1_c05600 0.58 +1.7 574,604 + PGA1_c05600 0.58 -0.1 574,657 - PGA1_c05600 0.66 +1.1 574,672 + PGA1_c05600 0.68 +0.6 574,693 + PGA1_c05600 0.71 +0.9 574,760 - PGA1_c05600 0.80 -1.1 574,768 + PGA1_c05600 0.82 -0.7 574,803 + PGA1_c05600 0.87 -0.9 574,852 + -1.0 574,988 + PGA1_c05610 0.26 +2.9 575,012 + PGA1_c05610 0.33 -1.1 575,017 + PGA1_c05610 0.34 +1.5 575,022 + PGA1_c05610 0.36 -0.9 575,030 - PGA1_c05610 0.38 -1.8 575,030 - PGA1_c05610 0.38 -0.9 575,030 - PGA1_c05610 0.38 -1.5 575,030 - PGA1_c05610 0.38 -0.9 575,035 - PGA1_c05610 0.39 -2.5 575,059 + PGA1_c05610 0.46 -0.4 575,106 + PGA1_c05610 0.59 +0.0 575,124 - PGA1_c05610 0.64 +0.9 575,139 + PGA1_c05610 0.69 -0.4 575,139 + PGA1_c05610 0.69 +1.9 575,139 + PGA1_c05610 0.69 -1.4 575,147 - PGA1_c05610 0.71 -0.4 575,147 - PGA1_c05610 0.71 +2.0 575,187 + PGA1_c05610 0.82 +1.9 575,237 + -0.5 575,322 + -0.1 575,377 + PGA1_c05620 0.16 -0.1 575,408 + PGA1_c05620 0.20 +1.2 575,432 + PGA1_c05620 0.23 +2.9 575,433 - PGA1_c05620 0.23 +1.9 575,465 - PGA1_c05620 0.27 +0.3 575,465 - PGA1_c05620 0.27 -0.9 575,520 + PGA1_c05620 0.34 -0.2 575,520 + PGA1_c05620 0.34 +1.9 575,525 + PGA1_c05620 0.35 -1.1 575,617 + PGA1_c05620 0.46 -1.4 575,617 + PGA1_c05620 0.46 +1.9 575,617 + PGA1_c05620 0.46 +1.9 575,651 + PGA1_c05620 0.51 -0.4 575,671 + PGA1_c05620 0.53 +1.9 575,684 - PGA1_c05620 0.55 +1.5 575,702 - PGA1_c05620 0.57 +0.9 575,761 + PGA1_c05620 0.65 +4.7 575,761 + PGA1_c05620 0.65 +0.3 575,779 - PGA1_c05620 0.67 +1.3 575,822 + PGA1_c05620 0.72 -0.2 575,884 + PGA1_c05620 0.80 -0.4 575,890 + PGA1_c05620 0.81 -1.2 575,979 + -0.4 575,987 - -0.7 576,016 + -0.7 576,070 + -0.7 576,101 + +0.1 576,109 + +1.9 576,117 - PGA1_c05630 0.10 +0.7 576,117 - PGA1_c05630 0.10 -1.4 576,117 - PGA1_c05630 0.10 -1.8 576,117 - PGA1_c05630 0.10 -0.1 576,117 - PGA1_c05630 0.10 -0.1 576,117 - PGA1_c05630 0.10 +0.1 576,122 - PGA1_c05630 0.11 +0.1 576,122 - PGA1_c05630 0.11 +0.3 576,138 - PGA1_c05630 0.15 -0.4 576,148 - PGA1_c05630 0.17 -1.1 576,174 + PGA1_c05630 0.23 +1.7 576,226 - PGA1_c05630 0.34 -0.7 576,226 - PGA1_c05630 0.34 -0.1 576,239 + PGA1_c05630 0.37 -0.1
Or see this region's nucleotide sequence