Experiment: m.b. Cisplatin 0.025 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_251 and HP15_252 overlap by 7 nucleotides HP15_252 and HP15_253 are separated by 183 nucleotides HP15_253 and HP15_254 are separated by 12 nucleotides HP15_254 and HP15_255 are separated by 260 nucleotides
HP15_251: HP15_251 - diguanylate cyclase, at 239,531 to 241,024
_251
HP15_252: HP15_252 - conserved hypothetical protein, at 241,018 to 241,257
_252
HP15_253: HP15_253 - extracellular solute-binding protein, family 3, at 241,441 to 244,149
_253
HP15_254: HP15_254 - conserved hypothetical protein, at 244,162 to 244,485
_254
HP15_255: HP15_255 - conserved hypothetical protein, at 244,746 to 245,351
_255
Position (kb)
241
242
243
244
245 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 240.444 kb on - strand, within HP15_251 at 240.444 kb on - strand, within HP15_251 at 240.451 kb on + strand, within HP15_251 at 240.472 kb on + strand, within HP15_251 at 240.485 kb on - strand, within HP15_251 at 240.489 kb on + strand, within HP15_251 at 240.509 kb on + strand, within HP15_251 at 240.544 kb on - strand, within HP15_251 at 240.553 kb on - strand, within HP15_251 at 240.559 kb on + strand, within HP15_251 at 240.610 kb on - strand, within HP15_251 at 240.629 kb on + strand, within HP15_251 at 240.642 kb on - strand, within HP15_251 at 240.672 kb on - strand, within HP15_251 at 240.921 kb on + strand at 240.967 kb on + strand at 240.981 kb on + strand at 240.991 kb on + strand at 241.000 kb on - strand at 241.016 kb on - strand at 241.120 kb on - strand, within HP15_252 at 241.120 kb on - strand, within HP15_252 at 241.333 kb on + strand at 241.392 kb on + strand at 241.454 kb on - strand at 241.559 kb on + strand at 241.630 kb on - strand at 241.644 kb on + strand at 241.672 kb on + strand at 241.680 kb on - strand at 241.695 kb on - strand at 241.877 kb on - strand, within HP15_253 at 242.174 kb on + strand, within HP15_253 at 242.194 kb on + strand at 242.194 kb on + strand, within HP15_253 at 242.212 kb on - strand, within HP15_253 at 242.418 kb on + strand, within HP15_253 at 242.624 kb on - strand, within HP15_253 at 242.657 kb on - strand, within HP15_253 at 242.762 kb on + strand, within HP15_253 at 242.784 kb on + strand, within HP15_253 at 242.789 kb on + strand, within HP15_253 at 242.789 kb on + strand, within HP15_253 at 242.789 kb on + strand, within HP15_253 at 242.789 kb on + strand, within HP15_253 at 242.789 kb on + strand, within HP15_253 at 242.797 kb on - strand, within HP15_253 at 242.797 kb on - strand, within HP15_253 at 242.797 kb on - strand, within HP15_253 at 242.797 kb on - strand, within HP15_253 at 242.826 kb on - strand, within HP15_253 at 242.826 kb on - strand, within HP15_253 at 242.904 kb on - strand, within HP15_253 at 243.055 kb on + strand, within HP15_253 at 243.055 kb on + strand, within HP15_253 at 243.093 kb on + strand, within HP15_253 at 243.199 kb on - strand, within HP15_253 at 243.263 kb on + strand, within HP15_253 at 243.271 kb on - strand, within HP15_253 at 243.386 kb on + strand, within HP15_253 at 243.388 kb on - strand, within HP15_253 at 243.394 kb on - strand, within HP15_253 at 243.398 kb on + strand, within HP15_253 at 243.519 kb on - strand, within HP15_253 at 243.521 kb on + strand, within HP15_253 at 243.810 kb on + strand, within HP15_253 at 243.813 kb on - strand, within HP15_253 at 243.823 kb on - strand, within HP15_253 at 243.956 kb on + strand at 243.956 kb on - strand at 244.040 kb on + strand at 244.110 kb on + strand at 244.111 kb on + strand at 244.111 kb on + strand at 244.111 kb on + strand at 244.199 kb on + strand, within HP15_254 at 244.230 kb on - strand, within HP15_254 at 244.270 kb on - strand, within HP15_254 at 244.286 kb on + strand, within HP15_254 at 244.286 kb on + strand, within HP15_254 at 244.293 kb on - strand, within HP15_254 at 244.294 kb on - strand, within HP15_254 at 244.296 kb on + strand, within HP15_254 at 244.366 kb on + strand, within HP15_254 at 244.403 kb on + strand, within HP15_254 at 244.715 kb on - strand at 244.779 kb on + strand at 244.823 kb on + strand, within HP15_255 at 244.823 kb on + strand, within HP15_255 at 244.831 kb on - strand, within HP15_255 at 245.030 kb on - strand, within HP15_255 at 245.030 kb on - strand, within HP15_255
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cisplatin 0.025 mg/ml remove 240,444 - HP15_251 0.61 -1.2 240,444 - HP15_251 0.61 -0.2 240,451 + HP15_251 0.62 +0.0 240,472 + HP15_251 0.63 +0.5 240,485 - HP15_251 0.64 +0.4 240,489 + HP15_251 0.64 -0.2 240,509 + HP15_251 0.65 +0.3 240,544 - HP15_251 0.68 -1.7 240,553 - HP15_251 0.68 +1.8 240,559 + HP15_251 0.69 +2.1 240,610 - HP15_251 0.72 +0.7 240,629 + HP15_251 0.73 -0.3 240,642 - HP15_251 0.74 +1.3 240,672 - HP15_251 0.76 -0.0 240,921 + +0.5 240,967 + +1.1 240,981 + -0.1 240,991 + +0.1 241,000 - +0.5 241,016 - +0.6 241,120 - HP15_252 0.42 -0.3 241,120 - HP15_252 0.42 -0.2 241,333 + -0.1 241,392 + -3.5 241,454 - -1.2 241,559 + -0.2 241,630 - -3.9 241,644 + -0.8 241,672 + -2.4 241,680 - -1.5 241,695 - -3.2 241,877 - HP15_253 0.16 -4.1 242,174 + HP15_253 0.27 -1.2 242,194 + -1.5 242,194 + HP15_253 0.28 -5.4 242,212 - HP15_253 0.28 -2.0 242,418 + HP15_253 0.36 -3.2 242,624 - HP15_253 0.44 +0.8 242,657 - HP15_253 0.45 -3.7 242,762 + HP15_253 0.49 -3.4 242,784 + HP15_253 0.50 -3.1 242,789 + HP15_253 0.50 -2.9 242,789 + HP15_253 0.50 -2.1 242,789 + HP15_253 0.50 -2.5 242,789 + HP15_253 0.50 -5.2 242,789 + HP15_253 0.50 -1.2 242,797 - HP15_253 0.50 -0.8 242,797 - HP15_253 0.50 -2.5 242,797 - HP15_253 0.50 -2.2 242,797 - HP15_253 0.50 -3.6 242,826 - HP15_253 0.51 -1.5 242,826 - HP15_253 0.51 -2.0 242,904 - HP15_253 0.54 -1.2 243,055 + HP15_253 0.60 -3.1 243,055 + HP15_253 0.60 -3.6 243,093 + HP15_253 0.61 -2.6 243,199 - HP15_253 0.65 -2.0 243,263 + HP15_253 0.67 -2.0 243,271 - HP15_253 0.68 -3.0 243,386 + HP15_253 0.72 -3.5 243,388 - HP15_253 0.72 -3.1 243,394 - HP15_253 0.72 -3.2 243,398 + HP15_253 0.72 -1.5 243,519 - HP15_253 0.77 -2.2 243,521 + HP15_253 0.77 -1.5 243,810 + HP15_253 0.87 -3.4 243,813 - HP15_253 0.88 -1.3 243,823 - HP15_253 0.88 -2.2 243,956 + -3.9 243,956 - -4.5 244,040 + -3.5 244,110 + +1.6 244,111 + -0.6 244,111 + +1.8 244,111 + +0.5 244,199 + HP15_254 0.11 +2.2 244,230 - HP15_254 0.21 +0.6 244,270 - HP15_254 0.33 +1.0 244,286 + HP15_254 0.38 +0.4 244,286 + HP15_254 0.38 +0.2 244,293 - HP15_254 0.40 +0.6 244,294 - HP15_254 0.41 +0.2 244,296 + HP15_254 0.41 +0.1 244,366 + HP15_254 0.63 +0.2 244,403 + HP15_254 0.74 -0.7 244,715 - +1.8 244,779 + -0.3 244,823 + HP15_255 0.13 +0.3 244,823 + HP15_255 0.13 +0.7 244,831 - HP15_255 0.14 -1.4 245,030 - HP15_255 0.47 +1.8 245,030 - HP15_255 0.47 -0.0
Or see this region's nucleotide sequence