Experiment: L-Lactate
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_1037 and ispD overlap by 4 nucleotides ispD and ispF are separated by 9 nucleotides ispF and Sama_1040 overlap by 4 nucleotides
Sama_1037: Sama_1037 - cell division protein FtsB (RefSeq), at 1,256,588 to 1,256,905
_1037
Sama_1038: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,256,902 to 1,257,618
ispD
Sama_1039: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,257,628 to 1,258,110
ispF
Sama_1040: Sama_1040 - pseudouridylate synthase (RefSeq), at 1,258,107 to 1,259,189
_1040
Position (kb)
1257
1258
1259 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1257.616 kb on + strand at 1258.122 kb on + strand at 1258.123 kb on - strand at 1258.123 kb on - strand at 1258.144 kb on + strand at 1258.144 kb on + strand at 1258.145 kb on - strand at 1258.244 kb on + strand, within Sama_1040 at 1258.244 kb on + strand, within Sama_1040 at 1258.245 kb on - strand, within Sama_1040 at 1258.350 kb on - strand, within Sama_1040 at 1258.429 kb on + strand, within Sama_1040 at 1258.430 kb on - strand, within Sama_1040 at 1258.451 kb on + strand, within Sama_1040 at 1258.451 kb on + strand, within Sama_1040 at 1258.452 kb on - strand, within Sama_1040 at 1258.491 kb on - strand, within Sama_1040 at 1258.491 kb on - strand, within Sama_1040 at 1258.572 kb on + strand, within Sama_1040 at 1258.572 kb on + strand, within Sama_1040 at 1258.648 kb on + strand, within Sama_1040 at 1258.648 kb on + strand, within Sama_1040 at 1258.674 kb on + strand, within Sama_1040 at 1258.703 kb on + strand, within Sama_1040 at 1258.703 kb on + strand, within Sama_1040 at 1258.704 kb on - strand, within Sama_1040 at 1258.704 kb on - strand, within Sama_1040 at 1258.704 kb on - strand, within Sama_1040 at 1258.794 kb on - strand, within Sama_1040 at 1258.823 kb on + strand, within Sama_1040 at 1258.824 kb on - strand, within Sama_1040 at 1258.932 kb on + strand, within Sama_1040 at 1258.932 kb on + strand, within Sama_1040 at 1259.103 kb on + strand at 1259.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate remove 1,257,616 + -0.5 1,258,122 + -1.0 1,258,123 - -1.0 1,258,123 - -1.2 1,258,144 + -0.0 1,258,144 + +0.9 1,258,145 - -2.0 1,258,244 + Sama_1040 0.13 +2.0 1,258,244 + Sama_1040 0.13 -2.6 1,258,245 - Sama_1040 0.13 -1.5 1,258,350 - Sama_1040 0.22 -1.5 1,258,429 + Sama_1040 0.30 -2.5 1,258,430 - Sama_1040 0.30 -3.0 1,258,451 + Sama_1040 0.32 -2.8 1,258,451 + Sama_1040 0.32 -0.2 1,258,452 - Sama_1040 0.32 -3.1 1,258,491 - Sama_1040 0.35 -0.2 1,258,491 - Sama_1040 0.35 -2.3 1,258,572 + Sama_1040 0.43 +0.5 1,258,572 + Sama_1040 0.43 -1.0 1,258,648 + Sama_1040 0.50 -0.2 1,258,648 + Sama_1040 0.50 -2.0 1,258,674 + Sama_1040 0.52 +0.7 1,258,703 + Sama_1040 0.55 -1.4 1,258,703 + Sama_1040 0.55 +0.7 1,258,704 - Sama_1040 0.55 -2.2 1,258,704 - Sama_1040 0.55 -2.9 1,258,704 - Sama_1040 0.55 -2.2 1,258,794 - Sama_1040 0.63 -2.5 1,258,823 + Sama_1040 0.66 +1.2 1,258,824 - Sama_1040 0.66 -3.0 1,258,932 + Sama_1040 0.76 -1.5 1,258,932 + Sama_1040 0.76 -0.2 1,259,103 + +1.7 1,259,103 + +1.3
Or see this region's nucleotide sequence