Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3370 and Psest_3371 overlap by 4 nucleotides Psest_3371 and Psest_3372 overlap by 4 nucleotides Psest_3372 and Psest_3373 overlap by 4 nucleotides Psest_3373 and Psest_3374 are separated by 4 nucleotides
Psest_3370: Psest_3370 - prepilin-type N-terminal cleavage/methylation domain, at 3,539,223 to 3,539,714
_3370
Psest_3371: Psest_3371 - type IV pilus modification protein PilV, at 3,539,711 to 3,540,181
_3371
Psest_3372: Psest_3372 - Tfp pilus assembly protein PilW, at 3,540,178 to 3,541,251
_3372
Psest_3373: Psest_3373 - Tfp pilus assembly protein PilX, at 3,541,248 to 3,541,781
_3373
Psest_3374: Psest_3374 - Tfp pilus assembly protein, tip-associated adhesin PilY1, at 3,541,786 to 3,545,556
_3374
Position (kb)
3540
3541
3542 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3539.278 kb on + strand, within Psest_3370 at 3539.278 kb on + strand, within Psest_3370 at 3539.279 kb on - strand, within Psest_3370 at 3539.324 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.377 kb on + strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.485 kb on + strand, within Psest_3370 at 3539.486 kb on - strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.724 kb on + strand at 3539.724 kb on + strand at 3539.725 kb on - strand at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.767 kb on - strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.869 kb on + strand, within Psest_3371 at 3539.874 kb on + strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3540.177 kb on + strand at 3540.178 kb on - strand at 3540.227 kb on + strand at 3540.228 kb on - strand at 3540.228 kb on - strand at 3540.270 kb on - strand at 3540.270 kb on - strand at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.312 kb on + strand, within Psest_3372 at 3540.313 kb on - strand, within Psest_3372 at 3540.463 kb on + strand, within Psest_3372 at 3540.464 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.645 kb on + strand, within Psest_3372 at 3540.653 kb on - strand, within Psest_3372 at 3540.794 kb on - strand, within Psest_3372 at 3540.804 kb on + strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.985 kb on + strand, within Psest_3372 at 3540.986 kb on - strand, within Psest_3372 at 3540.989 kb on + strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3541.011 kb on - strand, within Psest_3372 at 3541.038 kb on + strand, within Psest_3372 at 3541.162 kb on + strand at 3541.310 kb on + strand, within Psest_3373 at 3541.311 kb on - strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.581 kb on + strand, within Psest_3373 at 3541.582 kb on - strand, within Psest_3373 at 3541.699 kb on + strand, within Psest_3373 at 3541.700 kb on - strand, within Psest_3373 at 3541.700 kb on - strand, within Psest_3373 at 3541.731 kb on - strand at 3541.764 kb on + strand at 3541.765 kb on - strand at 3541.847 kb on + strand at 3541.920 kb on - strand at 3541.940 kb on + strand at 3542.011 kb on + strand at 3542.030 kb on - strand at 3542.128 kb on + strand at 3542.129 kb on - strand at 3542.134 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,539,278 + Psest_3370 0.11 +0.1 3,539,278 + Psest_3370 0.11 -0.4 3,539,279 - Psest_3370 0.11 +1.6 3,539,324 - Psest_3370 0.21 +2.3 3,539,361 - Psest_3370 0.28 +1.1 3,539,361 - Psest_3370 0.28 +2.3 3,539,361 - Psest_3370 0.28 +1.3 3,539,377 + Psest_3370 0.31 -0.6 3,539,378 - Psest_3370 0.32 +1.8 3,539,378 - Psest_3370 0.32 +0.8 3,539,378 - Psest_3370 0.32 -0.2 3,539,378 - Psest_3370 0.32 +0.9 3,539,485 + Psest_3370 0.53 +2.5 3,539,486 - Psest_3370 0.53 +1.4 3,539,561 + Psest_3370 0.69 +0.3 3,539,561 + Psest_3370 0.69 -0.0 3,539,561 + Psest_3370 0.69 -0.2 3,539,561 + Psest_3370 0.69 +2.2 3,539,561 + Psest_3370 0.69 -0.8 3,539,561 + Psest_3370 0.69 -2.0 3,539,562 - Psest_3370 0.69 +1.6 3,539,562 - Psest_3370 0.69 +0.5 3,539,562 - Psest_3370 0.69 +1.0 3,539,562 - Psest_3370 0.69 +0.1 3,539,562 - Psest_3370 0.69 +0.5 3,539,562 - Psest_3370 0.69 +1.4 3,539,562 - Psest_3370 0.69 +1.5 3,539,562 - Psest_3370 0.69 +1.1 3,539,562 - Psest_3370 0.69 +1.0 3,539,562 - Psest_3370 0.69 +1.1 3,539,562 - Psest_3370 0.69 +0.8 3,539,562 - Psest_3370 0.69 +1.4 3,539,562 - Psest_3370 0.69 +0.8 3,539,562 - Psest_3370 0.69 +1.5 3,539,724 + +1.1 3,539,724 + +0.5 3,539,725 - +1.3 3,539,766 + Psest_3371 0.12 +0.3 3,539,766 + Psest_3371 0.12 +0.4 3,539,766 + Psest_3371 0.12 +1.3 3,539,766 + Psest_3371 0.12 +1.3 3,539,767 - Psest_3371 0.12 +1.2 3,539,852 + Psest_3371 0.30 +1.5 3,539,852 + Psest_3371 0.30 +0.4 3,539,853 - Psest_3371 0.30 +1.3 3,539,853 - Psest_3371 0.30 +1.6 3,539,853 - Psest_3371 0.30 +0.2 3,539,855 + Psest_3371 0.31 +1.4 3,539,855 + Psest_3371 0.31 +0.4 3,539,869 + Psest_3371 0.34 +0.8 3,539,874 + Psest_3371 0.35 +1.1 3,539,883 - Psest_3371 0.37 +1.5 3,539,883 - Psest_3371 0.37 -0.6 3,539,936 + Psest_3371 0.48 +1.0 3,539,936 + Psest_3371 0.48 +0.1 3,539,937 - Psest_3371 0.48 +1.1 3,539,937 - Psest_3371 0.48 +0.9 3,540,177 + +0.6 3,540,178 - +1.0 3,540,227 + +0.3 3,540,228 - +0.9 3,540,228 - +0.9 3,540,270 - +0.5 3,540,270 - +1.4 3,540,301 + Psest_3372 0.11 +1.3 3,540,301 + Psest_3372 0.11 +1.7 3,540,301 + Psest_3372 0.11 +1.0 3,540,301 + Psest_3372 0.11 +1.0 3,540,301 + Psest_3372 0.11 +1.2 3,540,302 - Psest_3372 0.12 +1.4 3,540,302 - Psest_3372 0.12 +0.8 3,540,312 + Psest_3372 0.12 +1.1 3,540,313 - Psest_3372 0.13 +0.4 3,540,463 + Psest_3372 0.27 +1.1 3,540,464 - Psest_3372 0.27 +0.1 3,540,566 - Psest_3372 0.36 +0.9 3,540,566 - Psest_3372 0.36 +2.8 3,540,566 - Psest_3372 0.36 +0.9 3,540,645 + Psest_3372 0.43 +1.0 3,540,653 - Psest_3372 0.44 +0.7 3,540,794 - Psest_3372 0.57 +1.7 3,540,804 + Psest_3372 0.58 +1.2 3,540,836 - Psest_3372 0.61 +0.4 3,540,836 - Psest_3372 0.61 +1.1 3,540,836 - Psest_3372 0.61 +1.8 3,540,836 - Psest_3372 0.61 +0.4 3,540,836 - Psest_3372 0.61 +0.5 3,540,985 + Psest_3372 0.75 +0.8 3,540,986 - Psest_3372 0.75 +3.2 3,540,989 + Psest_3372 0.76 +1.0 3,540,990 - Psest_3372 0.76 +0.3 3,540,990 - Psest_3372 0.76 +1.4 3,541,011 - Psest_3372 0.78 +0.2 3,541,038 + Psest_3372 0.80 +0.8 3,541,162 + +0.8 3,541,310 + Psest_3373 0.12 +1.8 3,541,311 - Psest_3373 0.12 +0.8 3,541,581 + Psest_3373 0.62 +0.7 3,541,581 + Psest_3373 0.62 +1.0 3,541,581 + Psest_3373 0.62 +0.8 3,541,581 + Psest_3373 0.62 +0.8 3,541,582 - Psest_3373 0.63 +1.2 3,541,699 + Psest_3373 0.84 +1.0 3,541,700 - Psest_3373 0.85 +0.0 3,541,700 - Psest_3373 0.85 +1.3 3,541,731 - +0.5 3,541,764 + +1.1 3,541,765 - +1.0 3,541,847 + +0.4 3,541,920 - -1.3 3,541,940 + +0.7 3,542,011 + +1.1 3,542,030 - +0.3 3,542,128 + -0.4 3,542,129 - +1.5 3,542,134 - -0.3
Or see this region's nucleotide sequence