Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3369 and Psest_3370 are separated by 99 nucleotides Psest_3370 and Psest_3371 overlap by 4 nucleotides Psest_3371 and Psest_3372 overlap by 4 nucleotides
Psest_3369: Psest_3369 - prepilin-type N-terminal cleavage/methylation domain, at 3,538,587 to 3,539,123
_3369
Psest_3370: Psest_3370 - prepilin-type N-terminal cleavage/methylation domain, at 3,539,223 to 3,539,714
_3370
Psest_3371: Psest_3371 - type IV pilus modification protein PilV, at 3,539,711 to 3,540,181
_3371
Psest_3372: Psest_3372 - Tfp pilus assembly protein PilW, at 3,540,178 to 3,541,251
_3372
Position (kb)
3539
3540
3541 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3538.802 kb on + strand, within Psest_3369 at 3538.802 kb on + strand, within Psest_3369 at 3538.802 kb on + strand, within Psest_3369 at 3538.803 kb on - strand, within Psest_3369 at 3538.803 kb on - strand, within Psest_3369 at 3538.859 kb on + strand, within Psest_3369 at 3538.860 kb on - strand, within Psest_3369 at 3538.860 kb on - strand, within Psest_3369 at 3538.862 kb on - strand, within Psest_3369 at 3538.934 kb on + strand, within Psest_3369 at 3538.935 kb on - strand, within Psest_3369 at 3538.935 kb on - strand, within Psest_3369 at 3538.935 kb on - strand, within Psest_3369 at 3538.939 kb on - strand, within Psest_3369 at 3538.939 kb on - strand, within Psest_3369 at 3538.939 kb on - strand, within Psest_3369 at 3538.939 kb on - strand, within Psest_3369 at 3539.007 kb on - strand, within Psest_3369 at 3539.278 kb on + strand, within Psest_3370 at 3539.278 kb on + strand, within Psest_3370 at 3539.279 kb on - strand, within Psest_3370 at 3539.324 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.361 kb on - strand, within Psest_3370 at 3539.377 kb on + strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.378 kb on - strand, within Psest_3370 at 3539.485 kb on + strand, within Psest_3370 at 3539.486 kb on - strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.561 kb on + strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.562 kb on - strand, within Psest_3370 at 3539.724 kb on + strand at 3539.724 kb on + strand at 3539.725 kb on - strand at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.766 kb on + strand, within Psest_3371 at 3539.767 kb on - strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.852 kb on + strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.853 kb on - strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.855 kb on + strand, within Psest_3371 at 3539.869 kb on + strand, within Psest_3371 at 3539.874 kb on + strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.883 kb on - strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.936 kb on + strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3539.937 kb on - strand, within Psest_3371 at 3540.177 kb on + strand at 3540.178 kb on - strand at 3540.227 kb on + strand at 3540.228 kb on - strand at 3540.228 kb on - strand at 3540.270 kb on - strand at 3540.270 kb on - strand at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.301 kb on + strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.302 kb on - strand, within Psest_3372 at 3540.312 kb on + strand, within Psest_3372 at 3540.313 kb on - strand, within Psest_3372 at 3540.463 kb on + strand, within Psest_3372 at 3540.464 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.566 kb on - strand, within Psest_3372 at 3540.645 kb on + strand, within Psest_3372 at 3540.653 kb on - strand, within Psest_3372 at 3540.794 kb on - strand, within Psest_3372 at 3540.804 kb on + strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.836 kb on - strand, within Psest_3372 at 3540.985 kb on + strand, within Psest_3372 at 3540.986 kb on - strand, within Psest_3372 at 3540.989 kb on + strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3540.990 kb on - strand, within Psest_3372 at 3541.011 kb on - strand, within Psest_3372 at 3541.038 kb on + strand, within Psest_3372 at 3541.162 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 3,538,802 + Psest_3369 0.40 -0.6 3,538,802 + Psest_3369 0.40 -0.1 3,538,802 + Psest_3369 0.40 -0.4 3,538,803 - Psest_3369 0.40 +1.8 3,538,803 - Psest_3369 0.40 +0.3 3,538,859 + Psest_3369 0.51 -0.9 3,538,860 - Psest_3369 0.51 +0.3 3,538,860 - Psest_3369 0.51 +0.2 3,538,862 - Psest_3369 0.51 -0.5 3,538,934 + Psest_3369 0.65 -2.5 3,538,935 - Psest_3369 0.65 +2.3 3,538,935 - Psest_3369 0.65 -0.2 3,538,935 - Psest_3369 0.65 -0.2 3,538,939 - Psest_3369 0.66 +0.6 3,538,939 - Psest_3369 0.66 +1.7 3,538,939 - Psest_3369 0.66 +1.1 3,538,939 - Psest_3369 0.66 +1.0 3,539,007 - Psest_3369 0.78 -1.0 3,539,278 + Psest_3370 0.11 +0.1 3,539,278 + Psest_3370 0.11 -0.4 3,539,279 - Psest_3370 0.11 +1.6 3,539,324 - Psest_3370 0.21 +2.3 3,539,361 - Psest_3370 0.28 +1.1 3,539,361 - Psest_3370 0.28 +2.3 3,539,361 - Psest_3370 0.28 +1.3 3,539,377 + Psest_3370 0.31 -0.6 3,539,378 - Psest_3370 0.32 +1.8 3,539,378 - Psest_3370 0.32 +0.8 3,539,378 - Psest_3370 0.32 -0.2 3,539,378 - Psest_3370 0.32 +0.9 3,539,485 + Psest_3370 0.53 +2.5 3,539,486 - Psest_3370 0.53 +1.4 3,539,561 + Psest_3370 0.69 +0.3 3,539,561 + Psest_3370 0.69 -0.0 3,539,561 + Psest_3370 0.69 -0.2 3,539,561 + Psest_3370 0.69 +2.2 3,539,561 + Psest_3370 0.69 -0.8 3,539,561 + Psest_3370 0.69 -2.0 3,539,562 - Psest_3370 0.69 +1.6 3,539,562 - Psest_3370 0.69 +0.5 3,539,562 - Psest_3370 0.69 +1.0 3,539,562 - Psest_3370 0.69 +0.1 3,539,562 - Psest_3370 0.69 +0.5 3,539,562 - Psest_3370 0.69 +1.4 3,539,562 - Psest_3370 0.69 +1.5 3,539,562 - Psest_3370 0.69 +1.1 3,539,562 - Psest_3370 0.69 +1.0 3,539,562 - Psest_3370 0.69 +1.1 3,539,562 - Psest_3370 0.69 +0.8 3,539,562 - Psest_3370 0.69 +1.4 3,539,562 - Psest_3370 0.69 +0.8 3,539,562 - Psest_3370 0.69 +1.5 3,539,724 + +1.1 3,539,724 + +0.5 3,539,725 - +1.3 3,539,766 + Psest_3371 0.12 +0.3 3,539,766 + Psest_3371 0.12 +0.4 3,539,766 + Psest_3371 0.12 +1.3 3,539,766 + Psest_3371 0.12 +1.3 3,539,767 - Psest_3371 0.12 +1.2 3,539,852 + Psest_3371 0.30 +1.5 3,539,852 + Psest_3371 0.30 +0.4 3,539,853 - Psest_3371 0.30 +1.3 3,539,853 - Psest_3371 0.30 +1.6 3,539,853 - Psest_3371 0.30 +0.2 3,539,855 + Psest_3371 0.31 +1.4 3,539,855 + Psest_3371 0.31 +0.4 3,539,869 + Psest_3371 0.34 +0.8 3,539,874 + Psest_3371 0.35 +1.1 3,539,883 - Psest_3371 0.37 +1.5 3,539,883 - Psest_3371 0.37 -0.6 3,539,936 + Psest_3371 0.48 +1.0 3,539,936 + Psest_3371 0.48 +0.1 3,539,937 - Psest_3371 0.48 +1.1 3,539,937 - Psest_3371 0.48 +0.9 3,540,177 + +0.6 3,540,178 - +1.0 3,540,227 + +0.3 3,540,228 - +0.9 3,540,228 - +0.9 3,540,270 - +0.5 3,540,270 - +1.4 3,540,301 + Psest_3372 0.11 +1.3 3,540,301 + Psest_3372 0.11 +1.7 3,540,301 + Psest_3372 0.11 +1.0 3,540,301 + Psest_3372 0.11 +1.0 3,540,301 + Psest_3372 0.11 +1.2 3,540,302 - Psest_3372 0.12 +1.4 3,540,302 - Psest_3372 0.12 +0.8 3,540,312 + Psest_3372 0.12 +1.1 3,540,313 - Psest_3372 0.13 +0.4 3,540,463 + Psest_3372 0.27 +1.1 3,540,464 - Psest_3372 0.27 +0.1 3,540,566 - Psest_3372 0.36 +0.9 3,540,566 - Psest_3372 0.36 +2.8 3,540,566 - Psest_3372 0.36 +0.9 3,540,645 + Psest_3372 0.43 +1.0 3,540,653 - Psest_3372 0.44 +0.7 3,540,794 - Psest_3372 0.57 +1.7 3,540,804 + Psest_3372 0.58 +1.2 3,540,836 - Psest_3372 0.61 +0.4 3,540,836 - Psest_3372 0.61 +1.1 3,540,836 - Psest_3372 0.61 +1.8 3,540,836 - Psest_3372 0.61 +0.4 3,540,836 - Psest_3372 0.61 +0.5 3,540,985 + Psest_3372 0.75 +0.8 3,540,986 - Psest_3372 0.75 +3.2 3,540,989 + Psest_3372 0.76 +1.0 3,540,990 - Psest_3372 0.76 +0.3 3,540,990 - Psest_3372 0.76 +1.4 3,541,011 - Psest_3372 0.78 +0.2 3,541,038 + Psest_3372 0.80 +0.8 3,541,162 + +0.8
Or see this region's nucleotide sequence