Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2325 and Psest_2326 are separated by 58 nucleotides Psest_2326 and Psest_2327 are separated by 37 nucleotides Psest_2327 and Psest_2328 are separated by 190 nucleotides
Psest_2325: Psest_2325 - alpha-L-glutamate ligase-related protein, at 2,479,206 to 2,480,189
_2325
Psest_2326: Psest_2326 - aminodeoxychorismate synthase, component I, bacterial clade, at 2,480,248 to 2,481,594
_2326
Psest_2327: Psest_2327 - phosphoserine phosphatase/homoserine phosphotransferase bifunctional protein, at 2,481,632 to 2,482,249
_2327
Psest_2328: Psest_2328 - phosophoadenylyl-sulfate reductase (thioredoxin)/thioredoxin-dependent adenylylsulfate APS reductase, at 2,482,440 to 2,483,174
_2328
Position (kb)
2480
2481
2482 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.281 kb on + strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.282 kb on - strand at 2479.293 kb on + strand at 2479.294 kb on - strand at 2479.383 kb on + strand, within Psest_2325 at 2479.383 kb on + strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.384 kb on - strand, within Psest_2325 at 2479.521 kb on + strand, within Psest_2325 at 2479.522 kb on - strand, within Psest_2325 at 2479.597 kb on - strand, within Psest_2325 at 2479.597 kb on - strand, within Psest_2325 at 2479.674 kb on + strand, within Psest_2325 at 2479.674 kb on + strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.675 kb on - strand, within Psest_2325 at 2479.769 kb on + strand, within Psest_2325 at 2479.938 kb on + strand, within Psest_2325 at 2479.938 kb on + strand, within Psest_2325 at 2479.939 kb on - strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.959 kb on + strand, within Psest_2325 at 2479.960 kb on - strand, within Psest_2325 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.437 kb on - strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.542 kb on + strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.543 kb on - strand, within Psest_2326 at 2480.624 kb on - strand, within Psest_2326 at 2480.714 kb on + strand, within Psest_2326 at 2480.715 kb on - strand, within Psest_2326 at 2480.801 kb on - strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.830 kb on + strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.831 kb on - strand, within Psest_2326 at 2480.911 kb on + strand, within Psest_2326 at 2480.911 kb on + strand, within Psest_2326 at 2480.938 kb on - strand, within Psest_2326 at 2480.944 kb on + strand, within Psest_2326 at 2480.945 kb on - strand, within Psest_2326 at 2481.075 kb on + strand, within Psest_2326 at 2481.076 kb on - strand, within Psest_2326 at 2481.224 kb on - strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.391 kb on + strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.392 kb on - strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.409 kb on + strand, within Psest_2326 at 2481.410 kb on - strand, within Psest_2326 at 2481.410 kb on - strand, within Psest_2326 at 2481.410 kb on - strand, within Psest_2326 at 2481.789 kb on + strand, within Psest_2327 at 2481.789 kb on + strand, within Psest_2327 at 2481.790 kb on - strand, within Psest_2327 at 2481.790 kb on - strand, within Psest_2327 at 2481.790 kb on - strand, within Psest_2327 at 2481.790 kb on - strand, within Psest_2327 at 2481.819 kb on + strand, within Psest_2327 at 2481.819 kb on + strand, within Psest_2327 at 2481.819 kb on + strand, within Psest_2327 at 2481.819 kb on + strand, within Psest_2327 at 2481.819 kb on + strand, within Psest_2327 at 2481.820 kb on - strand, within Psest_2327 at 2481.820 kb on - strand, within Psest_2327 at 2481.876 kb on + strand, within Psest_2327 at 2481.969 kb on + strand, within Psest_2327 at 2481.970 kb on - strand, within Psest_2327 at 2482.127 kb on + strand, within Psest_2327 at 2482.127 kb on + strand, within Psest_2327 at 2482.127 kb on + strand, within Psest_2327 at 2482.128 kb on - strand, within Psest_2327 at 2482.241 kb on + strand at 2482.242 kb on - strand at 2482.323 kb on - strand at 2482.324 kb on + strand at 2482.351 kb on - strand at 2482.369 kb on + strand at 2482.369 kb on + strand at 2482.370 kb on - strand at 2482.370 kb on - strand at 2482.370 kb on - strand at 2482.370 kb on - strand at 2482.370 kb on - strand at 2482.373 kb on + strand at 2482.383 kb on + strand at 2482.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 2,479,281 + +0.1 2,479,281 + -0.4 2,479,281 + -0.7 2,479,281 + +0.2 2,479,282 - +0.1 2,479,282 - -0.6 2,479,282 - -1.0 2,479,282 - -1.1 2,479,282 - -0.6 2,479,293 + +0.1 2,479,294 - -1.2 2,479,383 + Psest_2325 0.18 -0.3 2,479,383 + Psest_2325 0.18 -0.4 2,479,384 - Psest_2325 0.18 -0.5 2,479,384 - Psest_2325 0.18 -0.6 2,479,384 - Psest_2325 0.18 -1.0 2,479,521 + Psest_2325 0.32 -0.2 2,479,522 - Psest_2325 0.32 -0.9 2,479,597 - Psest_2325 0.40 +0.0 2,479,597 - Psest_2325 0.40 -0.9 2,479,674 + Psest_2325 0.48 -0.5 2,479,674 + Psest_2325 0.48 -0.6 2,479,675 - Psest_2325 0.48 -1.4 2,479,675 - Psest_2325 0.48 -0.7 2,479,675 - Psest_2325 0.48 -0.6 2,479,675 - Psest_2325 0.48 -1.7 2,479,675 - Psest_2325 0.48 -1.6 2,479,769 + Psest_2325 0.57 +0.2 2,479,938 + Psest_2325 0.74 -0.3 2,479,938 + Psest_2325 0.74 -1.8 2,479,939 - Psest_2325 0.74 -0.6 2,479,959 + Psest_2325 0.77 -0.7 2,479,959 + Psest_2325 0.77 -0.8 2,479,959 + Psest_2325 0.77 -0.8 2,479,960 - Psest_2325 0.77 -0.3 2,480,437 - Psest_2326 0.14 -0.1 2,480,437 - Psest_2326 0.14 +0.2 2,480,437 - Psest_2326 0.14 +0.3 2,480,437 - Psest_2326 0.14 -0.1 2,480,542 + Psest_2326 0.22 -0.2 2,480,542 + Psest_2326 0.22 +1.1 2,480,542 + Psest_2326 0.22 +0.3 2,480,543 - Psest_2326 0.22 +0.4 2,480,543 - Psest_2326 0.22 -0.5 2,480,543 - Psest_2326 0.22 -0.1 2,480,624 - Psest_2326 0.28 -0.1 2,480,714 + Psest_2326 0.35 +0.8 2,480,715 - Psest_2326 0.35 -0.2 2,480,801 - Psest_2326 0.41 -0.8 2,480,830 + Psest_2326 0.43 +0.5 2,480,830 + Psest_2326 0.43 +0.4 2,480,830 + Psest_2326 0.43 -0.3 2,480,830 + Psest_2326 0.43 +1.5 2,480,830 + Psest_2326 0.43 -0.2 2,480,830 + Psest_2326 0.43 +0.3 2,480,830 + Psest_2326 0.43 -0.0 2,480,830 + Psest_2326 0.43 -0.3 2,480,830 + Psest_2326 0.43 -3.2 2,480,830 + Psest_2326 0.43 -0.7 2,480,830 + Psest_2326 0.43 +0.9 2,480,830 + Psest_2326 0.43 +0.2 2,480,830 + Psest_2326 0.43 -0.3 2,480,830 + Psest_2326 0.43 -0.2 2,480,831 - Psest_2326 0.43 -1.4 2,480,831 - Psest_2326 0.43 +0.2 2,480,831 - Psest_2326 0.43 +0.4 2,480,831 - Psest_2326 0.43 -0.0 2,480,911 + Psest_2326 0.49 +0.4 2,480,911 + Psest_2326 0.49 +1.2 2,480,938 - Psest_2326 0.51 +0.1 2,480,944 + Psest_2326 0.52 -0.3 2,480,945 - Psest_2326 0.52 +0.4 2,481,075 + Psest_2326 0.61 +0.1 2,481,076 - Psest_2326 0.61 +0.2 2,481,224 - Psest_2326 0.72 -0.1 2,481,391 + Psest_2326 0.85 -0.7 2,481,391 + Psest_2326 0.85 -0.2 2,481,391 + Psest_2326 0.85 +0.1 2,481,391 + Psest_2326 0.85 +0.7 2,481,391 + Psest_2326 0.85 +0.4 2,481,391 + Psest_2326 0.85 +0.9 2,481,392 - Psest_2326 0.85 -0.3 2,481,392 - Psest_2326 0.85 +0.5 2,481,392 - Psest_2326 0.85 +0.2 2,481,392 - Psest_2326 0.85 -0.6 2,481,392 - Psest_2326 0.85 +0.0 2,481,392 - Psest_2326 0.85 -0.8 2,481,409 + Psest_2326 0.86 -0.3 2,481,409 + Psest_2326 0.86 -0.5 2,481,409 + Psest_2326 0.86 -0.9 2,481,409 + Psest_2326 0.86 -0.5 2,481,409 + Psest_2326 0.86 -0.0 2,481,409 + Psest_2326 0.86 +0.0 2,481,409 + Psest_2326 0.86 -0.5 2,481,410 - Psest_2326 0.86 +0.0 2,481,410 - Psest_2326 0.86 +0.3 2,481,410 - Psest_2326 0.86 -0.8 2,481,789 + Psest_2327 0.25 -0.7 2,481,789 + Psest_2327 0.25 +1.5 2,481,790 - Psest_2327 0.26 -0.1 2,481,790 - Psest_2327 0.26 +0.1 2,481,790 - Psest_2327 0.26 +0.2 2,481,790 - Psest_2327 0.26 -0.4 2,481,819 + Psest_2327 0.30 -0.8 2,481,819 + Psest_2327 0.30 -0.2 2,481,819 + Psest_2327 0.30 +0.7 2,481,819 + Psest_2327 0.30 -1.5 2,481,819 + Psest_2327 0.30 -1.8 2,481,820 - Psest_2327 0.30 -1.8 2,481,820 - Psest_2327 0.30 +0.2 2,481,876 + Psest_2327 0.39 -1.3 2,481,969 + Psest_2327 0.55 -1.1 2,481,970 - Psest_2327 0.55 -1.9 2,482,127 + Psest_2327 0.80 -0.3 2,482,127 + Psest_2327 0.80 -0.5 2,482,127 + Psest_2327 0.80 -0.4 2,482,128 - Psest_2327 0.80 -1.9 2,482,241 + -0.6 2,482,242 - -0.5 2,482,323 - -2.4 2,482,324 + +0.1 2,482,351 - -1.9 2,482,369 + +0.1 2,482,369 + -1.4 2,482,370 - -1.9 2,482,370 - -2.8 2,482,370 - -2.2 2,482,370 - -3.4 2,482,370 - -3.9 2,482,373 + +0.1 2,482,383 + +0.4 2,482,425 + +0.4
Or see this region's nucleotide sequence