Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1678 and Psest_1679 are separated by 390 nucleotides Psest_1679 and Psest_1680 are separated by 559 nucleotides
Psest_1678: Psest_1678 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 1,785,074 to 1,786,093
_1678
Psest_1679: Psest_1679 - ribonuclease, Rne/Rng family, at 1,786,484 to 1,789,666
_1679
Psest_1680: Psest_1680 - pseudouridine synthase, RluA family, at 1,790,226 to 1,791,179
_1680
Position (kb)
1786
1787
1788
1789
1790 Strain fitness (log2 ratio)
-2
-1
0
1 at 1786.147 kb on - strand at 1786.147 kb on - strand at 1786.236 kb on + strand at 1786.236 kb on + strand at 1786.237 kb on - strand at 1786.336 kb on + strand at 1786.337 kb on - strand at 1786.337 kb on - strand at 1786.337 kb on - strand at 1786.338 kb on + strand at 1786.353 kb on - strand at 1786.383 kb on + strand at 1786.384 kb on - strand at 1786.410 kb on + strand at 1786.410 kb on + strand at 1786.410 kb on + strand at 1786.411 kb on - strand at 1786.595 kb on + strand at 1786.596 kb on - strand at 1786.707 kb on - strand at 1787.061 kb on - strand, within Psest_1679 at 1787.428 kb on + strand, within Psest_1679 at 1787.428 kb on + strand, within Psest_1679 at 1787.429 kb on - strand, within Psest_1679 at 1790.057 kb on - strand at 1790.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,786,147 - -0.2 1,786,147 - -0.1 1,786,236 + -0.1 1,786,236 + +0.0 1,786,237 - +0.9 1,786,336 + +0.2 1,786,337 - +0.1 1,786,337 - -0.2 1,786,337 - -0.4 1,786,338 + +0.6 1,786,353 - -0.4 1,786,383 + +0.1 1,786,384 - -1.0 1,786,410 + -1.0 1,786,410 + -1.0 1,786,410 + -1.9 1,786,411 - +0.4 1,786,595 + +1.4 1,786,596 - -0.5 1,786,707 - -0.0 1,787,061 - Psest_1679 0.18 -0.6 1,787,428 + Psest_1679 0.30 -0.4 1,787,428 + Psest_1679 0.30 -2.5 1,787,429 - Psest_1679 0.30 -1.6 1,790,057 - -0.6 1,790,188 - -1.4
Or see this region's nucleotide sequence