Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1674 and Psest_1675 are separated by 45 nucleotides Psest_1675 and Psest_1676 overlap by 4 nucleotides Psest_1676 and Psest_1677 are separated by 1 nucleotides Psest_1677 and Psest_1678 overlap by 4 nucleotides
Psest_1674: Psest_1674 - tetraacyldisaccharide 4'-kinase, at 1,782,612 to 1,783,616
_1674
Psest_1675: Psest_1675 - Uncharacterized conserved protein, at 1,783,662 to 1,783,847
_1675
Psest_1676: Psest_1676 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 1,783,844 to 1,784,608
_1676
Psest_1677: Psest_1677 - Protein-tyrosine-phosphatase, at 1,784,610 to 1,785,077
_1677
Psest_1678: Psest_1678 - UDP-N-acetylenolpyruvoylglucosamine reductase, at 1,785,074 to 1,786,093
_1678
Position (kb) 1784
1785
1786 Strain fitness (log2 ratio) -1
0
1
2 at 1783.614 kb on + strand at 1783.614 kb on + strand at 1783.614 kb on + strand at 1783.730 kb on + strand, within Psest_1675 at 1784.639 kb on + strand at 1784.971 kb on + strand, within Psest_1677 at 1784.985 kb on + strand, within Psest_1677 at 1784.985 kb on + strand, within Psest_1677 at 1785.061 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,783,614 + -0.4 1,783,614 + +0.4 1,783,614 + +0.8 1,783,730 + Psest_1675 0.37 -0.7 1,784,639 + +0.4 1,784,971 + Psest_1677 0.77 +1.0 1,784,985 + Psest_1677 0.80 +0.2 1,784,985 + Psest_1677 0.80 +1.9 1,785,061 + +0.5
Or see this region's nucleotide sequence