Experiment: L-Glutamine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1661 and Psest_1662 overlap by 4 nucleotides Psest_1662 and Psest_1663 are separated by 146 nucleotides Psest_1663 and Psest_1664 are separated by 146 nucleotides Psest_1664 and Psest_1665 overlap by 4 nucleotides
Psest_1661: Psest_1661 - Membrane-associated lipoprotein involved in thiamine biosynthesis, at 1,770,731 to 1,771,783
_1661
Psest_1662: Psest_1662 - Uncharacterized protein conserved in bacteria, at 1,771,780 to 1,772,007
_1662
Psest_1663: Psest_1663 - Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes, at 1,772,154 to 1,773,548
_1663
Psest_1664: Psest_1664 - Glycerophosphoryl diester phosphodiesterase, at 1,773,695 to 1,774,417
_1664
Psest_1665: Psest_1665 - hypothetical protein, at 1,774,414 to 1,774,722
_1665
Position (kb)
1772
1773
1774 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1771.647 kb on + strand, within Psest_1661 at 1772.127 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.151 kb on + strand at 1772.152 kb on - strand at 1772.164 kb on - strand at 1772.164 kb on - strand at 1772.637 kb on - strand, within Psest_1663 at 1772.641 kb on - strand, within Psest_1663 at 1772.824 kb on - strand, within Psest_1663 at 1773.045 kb on + strand, within Psest_1663 at 1773.069 kb on + strand, within Psest_1663 at 1773.117 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.198 kb on + strand, within Psest_1663 at 1773.199 kb on - strand, within Psest_1663 at 1773.462 kb on + strand at 1773.637 kb on + strand at 1773.637 kb on + strand at 1773.878 kb on + strand, within Psest_1664 at 1773.879 kb on - strand, within Psest_1664 at 1773.879 kb on - strand, within Psest_1664 at 1773.927 kb on + strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.928 kb on - strand, within Psest_1664 at 1773.950 kb on + strand, within Psest_1664 at 1773.950 kb on + strand, within Psest_1664 at 1773.951 kb on - strand, within Psest_1664 at 1774.133 kb on + strand, within Psest_1664 at 1774.134 kb on - strand, within Psest_1664 at 1774.197 kb on + strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.198 kb on - strand, within Psest_1664 at 1774.328 kb on + strand, within Psest_1664 at 1774.329 kb on - strand, within Psest_1664 at 1774.329 kb on - strand, within Psest_1664 at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.404 kb on + strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.405 kb on - strand at 1774.423 kb on + strand at 1774.424 kb on - strand at 1774.449 kb on + strand, within Psest_1665 at 1774.449 kb on + strand, within Psest_1665 at 1774.449 kb on + strand, within Psest_1665 at 1774.450 kb on - strand, within Psest_1665 at 1774.450 kb on - strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665 at 1774.496 kb on + strand, within Psest_1665
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N) remove 1,771,647 + Psest_1661 0.87 -0.0 1,772,127 + -0.3 1,772,151 + +1.7 1,772,151 + +1.5 1,772,151 + -0.4 1,772,151 + +1.5 1,772,151 + -0.6 1,772,152 - +1.0 1,772,164 - +0.5 1,772,164 - +0.6 1,772,637 - Psest_1663 0.35 +2.5 1,772,641 - Psest_1663 0.35 +1.5 1,772,824 - Psest_1663 0.48 +0.4 1,773,045 + Psest_1663 0.64 +1.7 1,773,069 + Psest_1663 0.66 +0.5 1,773,117 + Psest_1663 0.69 +2.2 1,773,198 + Psest_1663 0.75 -0.6 1,773,198 + Psest_1663 0.75 +0.1 1,773,198 + Psest_1663 0.75 +0.3 1,773,199 - Psest_1663 0.75 +0.2 1,773,462 + -0.0 1,773,637 + +0.8 1,773,637 + +0.3 1,773,878 + Psest_1664 0.25 -0.3 1,773,879 - Psest_1664 0.25 +1.1 1,773,879 - Psest_1664 0.25 -1.1 1,773,927 + Psest_1664 0.32 -0.8 1,773,928 - Psest_1664 0.32 +1.0 1,773,928 - Psest_1664 0.32 -2.1 1,773,928 - Psest_1664 0.32 -0.4 1,773,928 - Psest_1664 0.32 -0.8 1,773,950 + Psest_1664 0.35 +0.8 1,773,950 + Psest_1664 0.35 -0.9 1,773,951 - Psest_1664 0.35 -0.3 1,774,133 + Psest_1664 0.61 -0.7 1,774,134 - Psest_1664 0.61 +1.4 1,774,197 + Psest_1664 0.69 -1.0 1,774,198 - Psest_1664 0.70 +0.1 1,774,198 - Psest_1664 0.70 +0.1 1,774,198 - Psest_1664 0.70 -0.3 1,774,328 + Psest_1664 0.88 +0.1 1,774,329 - Psest_1664 0.88 -0.5 1,774,329 - Psest_1664 0.88 +0.4 1,774,404 + -0.4 1,774,404 + -0.7 1,774,404 + +1.0 1,774,404 + +0.0 1,774,404 + -1.5 1,774,404 + -0.6 1,774,404 + -0.5 1,774,404 + -0.4 1,774,405 - +1.5 1,774,405 - +0.1 1,774,405 - -0.8 1,774,405 - -0.5 1,774,405 - +0.6 1,774,423 + +0.2 1,774,424 - -0.4 1,774,449 + Psest_1665 0.11 +0.7 1,774,449 + Psest_1665 0.11 -0.0 1,774,449 + Psest_1665 0.11 +0.4 1,774,450 - Psest_1665 0.12 -0.2 1,774,450 - Psest_1665 0.12 -0.4 1,774,496 + Psest_1665 0.27 -0.6 1,774,496 + Psest_1665 0.27 -0.7 1,774,496 + Psest_1665 0.27 -0.8
Or see this region's nucleotide sequence