Experiment: m.b. Phosphomycin 1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c02000 and PGA1_c02010 are separated by 12 nucleotides PGA1_c02010 and PGA1_c02020 are separated by 148 nucleotides PGA1_c02020 and PGA1_c02030 are separated by 218 nucleotides PGA1_c02030 and PGA1_c02040 overlap by 4 nucleotides
PGA1_c02000: PGA1_c02000 - glutamate/glutamine/aspartate/asparagine transport system permease protein BztC, at 182,984 to 184,282
_c02000
PGA1_c02010: PGA1_c02010 - glutamate/glutamine/aspartate/asparagine transport ATP-binding protein BztD, at 184,295 to 185,086
_c02010
PGA1_c02020: PGA1_c02020 - Predicted metal-binding protein, at 185,235 to 185,693
_c02020
PGA1_c02030: PGA1_c02030 - phosphoglycerate mutase-like protein, at 185,912 to 186,409
_c02030
PGA1_c02040: PGA1_c02040 - hypothetical protein, at 186,406 to 187,149
_c02040
Position (kb) 185
186 Strain fitness (log2 ratio) -2
-1
0
1
2 at 184.247 kb on - strand at 184.276 kb on + strand at 184.276 kb on + strand at 184.279 kb on - strand at 184.284 kb on - strand at 184.284 kb on - strand at 184.476 kb on + strand, within PGA1_c02010 at 184.530 kb on - strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.542 kb on + strand, within PGA1_c02010 at 184.550 kb on - strand, within PGA1_c02010 at 184.555 kb on - strand, within PGA1_c02010 at 184.584 kb on + strand, within PGA1_c02010 at 184.584 kb on + strand, within PGA1_c02010 at 184.592 kb on - strand, within PGA1_c02010 at 184.592 kb on - strand, within PGA1_c02010 at 184.611 kb on + strand, within PGA1_c02010 at 184.620 kb on - strand, within PGA1_c02010 at 184.621 kb on + strand, within PGA1_c02010 at 184.663 kb on + strand, within PGA1_c02010 at 184.694 kb on + strand, within PGA1_c02010 at 184.768 kb on + strand, within PGA1_c02010 at 184.773 kb on - strand, within PGA1_c02010 at 184.773 kb on - strand, within PGA1_c02010 at 184.775 kb on + strand, within PGA1_c02010 at 184.775 kb on + strand, within PGA1_c02010 at 184.778 kb on - strand, within PGA1_c02010 at 184.786 kb on + strand, within PGA1_c02010 at 184.856 kb on - strand, within PGA1_c02010 at 184.912 kb on + strand, within PGA1_c02010 at 184.919 kb on + strand, within PGA1_c02010 at 184.929 kb on + strand, within PGA1_c02010 at 185.082 kb on + strand at 185.090 kb on - strand at 185.215 kb on + strand at 185.253 kb on + strand at 185.285 kb on - strand, within PGA1_c02020 at 185.323 kb on - strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on + strand, within PGA1_c02020 at 185.438 kb on - strand, within PGA1_c02020 at 185.446 kb on - strand, within PGA1_c02020 at 185.537 kb on + strand, within PGA1_c02020 at 185.545 kb on - strand, within PGA1_c02020 at 185.553 kb on - strand, within PGA1_c02020 at 185.688 kb on + strand at 185.717 kb on + strand at 185.717 kb on + strand at 185.725 kb on - strand at 185.736 kb on - strand at 185.911 kb on - strand at 185.913 kb on - strand at 186.010 kb on + strand, within PGA1_c02030 at 186.124 kb on + strand, within PGA1_c02030 at 186.246 kb on - strand, within PGA1_c02030 at 186.353 kb on + strand, within PGA1_c02030 at 186.391 kb on + strand at 186.457 kb on + strand at 186.527 kb on + strand, within PGA1_c02040 at 186.535 kb on - strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.610 kb on + strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.618 kb on - strand, within PGA1_c02040 at 186.625 kb on + strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.651 kb on - strand, within PGA1_c02040 at 186.656 kb on - strand, within PGA1_c02040
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Phosphomycin 1 mg/ml remove 184,247 - -0.1 184,276 + -0.6 184,276 + -0.1 184,279 - +1.5 184,284 - +1.3 184,284 - -0.5 184,476 + PGA1_c02010 0.23 +0.6 184,530 - PGA1_c02010 0.30 +1.4 184,542 + PGA1_c02010 0.31 -0.6 184,542 + PGA1_c02010 0.31 +0.3 184,542 + PGA1_c02010 0.31 -0.7 184,542 + PGA1_c02010 0.31 -0.3 184,542 + PGA1_c02010 0.31 -1.0 184,550 - PGA1_c02010 0.32 +0.2 184,555 - PGA1_c02010 0.33 +1.7 184,584 + PGA1_c02010 0.36 -0.0 184,584 + PGA1_c02010 0.36 +0.3 184,592 - PGA1_c02010 0.38 -0.0 184,592 - PGA1_c02010 0.38 -0.3 184,611 + PGA1_c02010 0.40 -0.8 184,620 - PGA1_c02010 0.41 +0.3 184,621 + PGA1_c02010 0.41 -0.6 184,663 + PGA1_c02010 0.46 +0.5 184,694 + PGA1_c02010 0.50 +1.0 184,768 + PGA1_c02010 0.60 -0.7 184,773 - PGA1_c02010 0.60 +1.9 184,773 - PGA1_c02010 0.60 +1.1 184,775 + PGA1_c02010 0.61 +0.4 184,775 + PGA1_c02010 0.61 +0.9 184,778 - PGA1_c02010 0.61 -2.1 184,786 + PGA1_c02010 0.62 +0.2 184,856 - PGA1_c02010 0.71 +0.5 184,912 + PGA1_c02010 0.78 +0.2 184,919 + PGA1_c02010 0.79 -0.3 184,929 + PGA1_c02010 0.80 -0.7 185,082 + -1.8 185,090 - +0.2 185,215 + +0.3 185,253 + -0.5 185,285 - PGA1_c02020 0.11 -0.8 185,323 - PGA1_c02020 0.19 -1.1 185,438 + PGA1_c02020 0.44 -0.7 185,438 + PGA1_c02020 0.44 -0.1 185,438 - PGA1_c02020 0.44 -0.5 185,446 - PGA1_c02020 0.46 -0.0 185,537 + PGA1_c02020 0.66 -0.8 185,545 - PGA1_c02020 0.68 -1.0 185,553 - PGA1_c02020 0.69 +0.1 185,688 + +1.6 185,717 + -0.8 185,717 + -0.5 185,725 - +0.9 185,736 - +0.2 185,911 - +2.1 185,913 - +0.2 186,010 + PGA1_c02030 0.20 +1.1 186,124 + PGA1_c02030 0.43 +1.4 186,246 - PGA1_c02030 0.67 +0.9 186,353 + PGA1_c02030 0.89 +0.5 186,391 + +0.9 186,457 + +0.3 186,527 + PGA1_c02040 0.16 +1.3 186,535 - PGA1_c02040 0.17 +0.5 186,610 + PGA1_c02040 0.27 -0.3 186,610 + PGA1_c02040 0.27 +1.5 186,610 + PGA1_c02040 0.27 -0.5 186,618 - PGA1_c02040 0.28 +0.5 186,618 - PGA1_c02040 0.28 +1.0 186,618 - PGA1_c02040 0.28 +1.2 186,625 + PGA1_c02040 0.29 +1.3 186,651 - PGA1_c02040 0.33 -1.7 186,651 - PGA1_c02040 0.33 +0.1 186,656 - PGA1_c02040 0.34 +0.4
Or see this region's nucleotide sequence