Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nrfA and Sama_0649 are separated by 80 nucleotides Sama_0649 and Sama_0650 are separated by 240 nucleotides Sama_0650 and Sama_0651 are separated by 11 nucleotides
Sama_0648: nrfA - cytochrome c552 (RefSeq), at 803,874 to 805,277
nrfA
Sama_0649: Sama_0649 - isochorismatase family protein (RefSeq), at 805,358 to 805,897
_0649
Sama_0650: Sama_0650 - nitrogen regulatory protein P-II (RefSeq), at 806,138 to 806,476
_0650
Sama_0651: Sama_0651 - Rh-like protein/ammonium transporter (RefSeq), at 806,488 to 807,738
_0651
Position (kb) 806
807 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 805.145 kb on + strand at 805.145 kb on + strand at 805.145 kb on + strand at 805.146 kb on - strand at 805.269 kb on + strand at 805.269 kb on + strand at 805.269 kb on + strand at 805.269 kb on + strand at 805.269 kb on + strand at 805.269 kb on + strand at 805.270 kb on - strand at 805.270 kb on - strand at 805.270 kb on - strand at 805.270 kb on - strand at 805.270 kb on - strand at 805.275 kb on + strand at 805.275 kb on + strand at 805.275 kb on + strand at 805.275 kb on + strand at 805.276 kb on - strand at 805.276 kb on - strand at 805.276 kb on - strand at 805.276 kb on - strand at 805.276 kb on - strand at 805.299 kb on + strand at 805.300 kb on - strand at 805.300 kb on - strand at 805.347 kb on - strand at 805.350 kb on - strand at 805.350 kb on - strand at 805.350 kb on - strand at 805.359 kb on + strand at 805.359 kb on + strand at 805.359 kb on + strand at 805.360 kb on - strand at 805.360 kb on - strand at 805.360 kb on - strand at 805.472 kb on + strand, within Sama_0649 at 805.472 kb on + strand, within Sama_0649 at 805.569 kb on + strand, within Sama_0649 at 805.569 kb on + strand, within Sama_0649 at 805.570 kb on - strand, within Sama_0649 at 805.570 kb on - strand, within Sama_0649 at 805.589 kb on + strand, within Sama_0649 at 805.589 kb on + strand, within Sama_0649 at 805.590 kb on - strand, within Sama_0649 at 805.622 kb on - strand, within Sama_0649 at 805.742 kb on + strand, within Sama_0649 at 805.766 kb on + strand, within Sama_0649 at 805.766 kb on + strand, within Sama_0649 at 805.766 kb on + strand, within Sama_0649 at 805.766 kb on + strand, within Sama_0649 at 805.766 kb on + strand, within Sama_0649 at 805.767 kb on - strand, within Sama_0649 at 805.767 kb on - strand, within Sama_0649 at 805.767 kb on - strand, within Sama_0649 at 805.768 kb on + strand, within Sama_0649 at 805.768 kb on + strand, within Sama_0649 at 805.769 kb on - strand, within Sama_0649 at 805.769 kb on - strand, within Sama_0649 at 805.790 kb on + strand, within Sama_0649 at 805.790 kb on + strand, within Sama_0649 at 805.830 kb on + strand, within Sama_0649 at 805.830 kb on + strand, within Sama_0649 at 805.830 kb on + strand, within Sama_0649 at 805.830 kb on + strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.831 kb on - strand, within Sama_0649 at 805.891 kb on + strand at 805.892 kb on - strand at 805.892 kb on - strand at 805.892 kb on - strand at 805.899 kb on + strand at 805.900 kb on - strand at 805.900 kb on - strand at 805.925 kb on - strand at 805.925 kb on - strand at 805.936 kb on - strand at 805.950 kb on + strand at 805.993 kb on - strand at 805.993 kb on - strand at 806.046 kb on + strand at 806.047 kb on - strand at 806.114 kb on + strand at 806.114 kb on + strand at 806.114 kb on + strand at 806.114 kb on + strand at 806.114 kb on + strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.115 kb on - strand at 806.213 kb on + strand, within Sama_0650 at 806.218 kb on + strand, within Sama_0650 at 806.218 kb on + strand, within Sama_0650 at 806.218 kb on + strand, within Sama_0650 at 806.219 kb on - strand, within Sama_0650 at 806.219 kb on - strand, within Sama_0650 at 806.219 kb on - strand, within Sama_0650 at 806.226 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.271 kb on + strand, within Sama_0650 at 806.272 kb on - strand, within Sama_0650 at 806.272 kb on - strand, within Sama_0650 at 806.272 kb on - strand, within Sama_0650 at 806.272 kb on - strand, within Sama_0650 at 806.288 kb on + strand, within Sama_0650 at 806.289 kb on - strand, within Sama_0650 at 806.289 kb on - strand, within Sama_0650 at 806.313 kb on + strand, within Sama_0650 at 806.313 kb on + strand, within Sama_0650 at 806.314 kb on - strand, within Sama_0650 at 806.329 kb on + strand, within Sama_0650 at 806.330 kb on - strand, within Sama_0650 at 806.370 kb on + strand, within Sama_0650 at 806.404 kb on + strand, within Sama_0650 at 806.425 kb on - strand, within Sama_0650 at 806.474 kb on + strand at 806.474 kb on + strand at 806.474 kb on + strand at 806.474 kb on + strand at 806.474 kb on + strand at 806.474 kb on + strand at 806.475 kb on - strand at 806.545 kb on - strand at 806.552 kb on - strand at 806.668 kb on + strand, within Sama_0651 at 806.669 kb on - strand, within Sama_0651 at 806.683 kb on - strand, within Sama_0651 at 806.696 kb on + strand, within Sama_0651 at 806.696 kb on + strand, within Sama_0651 at 806.696 kb on + strand, within Sama_0651 at 806.697 kb on - strand, within Sama_0651 at 806.705 kb on - strand, within Sama_0651 at 806.705 kb on - strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.716 kb on + strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.717 kb on - strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.720 kb on + strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.721 kb on - strand, within Sama_0651 at 806.825 kb on + strand, within Sama_0651 at 806.825 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.841 kb on + strand, within Sama_0651 at 806.842 kb on - strand, within Sama_0651 at 806.842 kb on - strand, within Sama_0651 at 806.842 kb on - strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.922 kb on + strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.923 kb on - strand, within Sama_0651 at 806.926 kb on + strand, within Sama_0651 at 806.926 kb on + strand, within Sama_0651 at 806.927 kb on - strand, within Sama_0651 at 807.003 kb on + strand, within Sama_0651 at 807.003 kb on + strand, within Sama_0651 at 807.003 kb on + strand, within Sama_0651 at 807.004 kb on - strand, within Sama_0651 at 807.069 kb on + strand, within Sama_0651 at 807.069 kb on + strand, within Sama_0651 at 807.069 kb on + strand, within Sama_0651 at 807.070 kb on - strand, within Sama_0651 at 807.070 kb on - strand, within Sama_0651 at 807.070 kb on - strand, within Sama_0651 at 807.070 kb on - strand, within Sama_0651 at 807.070 kb on - strand, within Sama_0651 at 807.073 kb on + strand, within Sama_0651 at 807.073 kb on + strand, within Sama_0651 at 807.073 kb on + strand, within Sama_0651 at 807.073 kb on + strand, within Sama_0651 at 807.074 kb on - strand, within Sama_0651 at 807.136 kb on - strand, within Sama_0651 at 807.141 kb on + strand, within Sama_0651 at 807.223 kb on - strand, within Sama_0651 at 807.237 kb on + strand, within Sama_0651 at 807.311 kb on + strand, within Sama_0651 at 807.311 kb on + strand, within Sama_0651 at 807.311 kb on + strand, within Sama_0651 at 807.432 kb on + strand, within Sama_0651 at 807.433 kb on - strand, within Sama_0651 at 807.433 kb on - strand, within Sama_0651 at 807.433 kb on - strand, within Sama_0651 at 807.471 kb on - strand, within Sama_0651 at 807.471 kb on - strand, within Sama_0651
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 805,145 + -1.9 805,145 + +0.1 805,145 + -0.5 805,146 - +1.4 805,269 + +1.1 805,269 + -1.7 805,269 + +0.5 805,269 + -0.7 805,269 + +0.3 805,269 + +0.3 805,270 - +1.6 805,270 - -0.8 805,270 - +0.6 805,270 - +0.1 805,270 - -0.1 805,275 + -1.4 805,275 + -2.3 805,275 + -0.9 805,275 + +0.9 805,276 - -2.6 805,276 - -0.7 805,276 - -0.0 805,276 - +0.9 805,276 - -1.2 805,299 + +0.1 805,300 - +0.4 805,300 - -0.4 805,347 - +0.7 805,350 - -3.1 805,350 - -2.6 805,350 - +2.6 805,359 + +0.4 805,359 + +2.6 805,359 + -1.4 805,360 - -0.6 805,360 - -0.1 805,360 - -0.1 805,472 + Sama_0649 0.21 -0.3 805,472 + Sama_0649 0.21 -1.4 805,569 + Sama_0649 0.39 -2.0 805,569 + Sama_0649 0.39 +2.0 805,570 - Sama_0649 0.39 -1.9 805,570 - Sama_0649 0.39 -2.2 805,589 + Sama_0649 0.43 -0.9 805,589 + Sama_0649 0.43 +1.3 805,590 - Sama_0649 0.43 -1.7 805,622 - Sama_0649 0.49 +1.1 805,742 + Sama_0649 0.71 -1.0 805,766 + Sama_0649 0.76 +1.2 805,766 + Sama_0649 0.76 +1.3 805,766 + Sama_0649 0.76 -3.4 805,766 + Sama_0649 0.76 -0.4 805,766 + Sama_0649 0.76 +0.2 805,767 - Sama_0649 0.76 -0.4 805,767 - Sama_0649 0.76 +0.1 805,767 - Sama_0649 0.76 +1.4 805,768 + Sama_0649 0.76 +1.4 805,768 + Sama_0649 0.76 +0.8 805,769 - Sama_0649 0.76 -0.1 805,769 - Sama_0649 0.76 +2.1 805,790 + Sama_0649 0.80 +0.5 805,790 + Sama_0649 0.80 +0.1 805,830 + Sama_0649 0.87 +1.1 805,830 + Sama_0649 0.87 +0.1 805,830 + Sama_0649 0.87 +0.6 805,830 + Sama_0649 0.87 -1.4 805,831 - Sama_0649 0.88 +2.1 805,831 - Sama_0649 0.88 +1.3 805,831 - Sama_0649 0.88 +2.1 805,831 - Sama_0649 0.88 +0.4 805,831 - Sama_0649 0.88 -1.2 805,831 - Sama_0649 0.88 -1.4 805,831 - Sama_0649 0.88 +0.6 805,891 + +1.5 805,892 - +1.9 805,892 - +0.3 805,892 - -1.9 805,899 + -1.3 805,900 - -1.0 805,900 - -0.2 805,925 - +0.1 805,925 - -0.9 805,936 - +0.3 805,950 + +0.1 805,993 - -2.2 805,993 - +0.8 806,046 + +0.7 806,047 - -1.4 806,114 + +1.4 806,114 + +0.1 806,114 + -1.7 806,114 + +1.5 806,114 + -0.4 806,115 - -2.3 806,115 - +0.8 806,115 - +3.6 806,115 - -2.2 806,115 - -2.2 806,115 - -1.7 806,115 - +0.8 806,213 + Sama_0650 0.22 -3.1 806,218 + Sama_0650 0.24 +0.3 806,218 + Sama_0650 0.24 +1.3 806,218 + Sama_0650 0.24 -1.4 806,219 - Sama_0650 0.24 +0.6 806,219 - Sama_0650 0.24 -2.7 806,219 - Sama_0650 0.24 +0.6 806,226 + Sama_0650 0.26 -1.7 806,271 + Sama_0650 0.39 +1.3 806,271 + Sama_0650 0.39 -0.3 806,271 + Sama_0650 0.39 +0.8 806,271 + Sama_0650 0.39 -1.7 806,271 + Sama_0650 0.39 -0.6 806,271 + Sama_0650 0.39 +1.3 806,271 + Sama_0650 0.39 +0.7 806,272 - Sama_0650 0.40 -2.4 806,272 - Sama_0650 0.40 +0.7 806,272 - Sama_0650 0.40 -0.1 806,272 - Sama_0650 0.40 -1.0 806,288 + Sama_0650 0.44 +1.1 806,289 - Sama_0650 0.45 +0.3 806,289 - Sama_0650 0.45 +1.1 806,313 + Sama_0650 0.52 +1.4 806,313 + Sama_0650 0.52 +1.2 806,314 - Sama_0650 0.52 +2.9 806,329 + Sama_0650 0.56 +0.3 806,330 - Sama_0650 0.57 -0.9 806,370 + Sama_0650 0.68 +0.0 806,404 + Sama_0650 0.78 -0.5 806,425 - Sama_0650 0.85 -0.4 806,474 + +0.1 806,474 + -2.9 806,474 + +0.3 806,474 + -1.2 806,474 + -0.1 806,474 + -0.4 806,475 - -1.2 806,545 - +0.5 806,552 - +0.9 806,668 + Sama_0651 0.14 +1.1 806,669 - Sama_0651 0.14 +0.4 806,683 - Sama_0651 0.16 -1.2 806,696 + Sama_0651 0.17 -2.8 806,696 + Sama_0651 0.17 -1.4 806,696 + Sama_0651 0.17 +0.3 806,697 - Sama_0651 0.17 -0.3 806,705 - Sama_0651 0.17 +1.8 806,705 - Sama_0651 0.17 -2.2 806,716 + Sama_0651 0.18 +0.7 806,716 + Sama_0651 0.18 +0.7 806,716 + Sama_0651 0.18 +1.0 806,716 + Sama_0651 0.18 -0.4 806,716 + Sama_0651 0.18 +0.6 806,716 + Sama_0651 0.18 +0.4 806,716 + Sama_0651 0.18 +0.1 806,716 + Sama_0651 0.18 -0.9 806,716 + Sama_0651 0.18 +1.7 806,716 + Sama_0651 0.18 +0.9 806,716 + Sama_0651 0.18 -0.4 806,716 + Sama_0651 0.18 -0.1 806,716 + Sama_0651 0.18 -2.4 806,716 + Sama_0651 0.18 -1.4 806,716 + Sama_0651 0.18 +1.6 806,716 + Sama_0651 0.18 -0.5 806,716 + Sama_0651 0.18 +0.5 806,716 + Sama_0651 0.18 +0.4 806,716 + Sama_0651 0.18 -0.1 806,716 + Sama_0651 0.18 -0.3 806,716 + Sama_0651 0.18 -0.5 806,716 + Sama_0651 0.18 -0.3 806,716 + Sama_0651 0.18 +1.5 806,716 + Sama_0651 0.18 -0.8 806,716 + Sama_0651 0.18 -0.4 806,716 + Sama_0651 0.18 +0.9 806,716 + Sama_0651 0.18 -0.3 806,717 - Sama_0651 0.18 +0.3 806,717 - Sama_0651 0.18 -0.1 806,717 - Sama_0651 0.18 -1.2 806,717 - Sama_0651 0.18 -2.0 806,717 - Sama_0651 0.18 -2.4 806,717 - Sama_0651 0.18 -1.0 806,717 - Sama_0651 0.18 -2.0 806,717 - Sama_0651 0.18 -0.0 806,717 - Sama_0651 0.18 +0.9 806,717 - Sama_0651 0.18 +0.9 806,717 - Sama_0651 0.18 -1.9 806,717 - Sama_0651 0.18 -0.3 806,717 - Sama_0651 0.18 -0.3 806,717 - Sama_0651 0.18 +0.3 806,717 - Sama_0651 0.18 -0.1 806,720 + Sama_0651 0.19 -1.9 806,720 + Sama_0651 0.19 -2.0 806,720 + Sama_0651 0.19 +1.4 806,720 + Sama_0651 0.19 +1.4 806,720 + Sama_0651 0.19 -1.4 806,720 + Sama_0651 0.19 +1.6 806,720 + Sama_0651 0.19 +0.1 806,720 + Sama_0651 0.19 -2.0 806,721 - Sama_0651 0.19 +0.6 806,721 - Sama_0651 0.19 -0.4 806,721 - Sama_0651 0.19 +1.7 806,721 - Sama_0651 0.19 +0.1 806,721 - Sama_0651 0.19 +1.0 806,721 - Sama_0651 0.19 +0.1 806,825 + Sama_0651 0.27 -2.7 806,825 + Sama_0651 0.27 -1.4 806,841 + Sama_0651 0.28 -1.0 806,841 + Sama_0651 0.28 +1.6 806,841 + Sama_0651 0.28 -1.6 806,841 + Sama_0651 0.28 +0.1 806,841 + Sama_0651 0.28 +0.0 806,841 + Sama_0651 0.28 -1.2 806,842 - Sama_0651 0.28 -0.9 806,842 - Sama_0651 0.28 -0.2 806,842 - Sama_0651 0.28 -3.2 806,922 + Sama_0651 0.35 -0.3 806,922 + Sama_0651 0.35 -2.0 806,922 + Sama_0651 0.35 +1.7 806,922 + Sama_0651 0.35 +1.0 806,922 + Sama_0651 0.35 -2.6 806,922 + Sama_0651 0.35 +0.0 806,922 + Sama_0651 0.35 -1.7 806,922 + Sama_0651 0.35 +0.3 806,922 + Sama_0651 0.35 -0.3 806,922 + Sama_0651 0.35 -1.2 806,923 - Sama_0651 0.35 +1.3 806,923 - Sama_0651 0.35 +0.1 806,923 - Sama_0651 0.35 +0.4 806,923 - Sama_0651 0.35 -1.7 806,923 - Sama_0651 0.35 +2.8 806,923 - Sama_0651 0.35 -1.6 806,923 - Sama_0651 0.35 -0.4 806,923 - Sama_0651 0.35 +1.3 806,926 + Sama_0651 0.35 -2.0 806,926 + Sama_0651 0.35 +1.9 806,927 - Sama_0651 0.35 +0.7 807,003 + Sama_0651 0.41 -0.9 807,003 + Sama_0651 0.41 +0.5 807,003 + Sama_0651 0.41 -0.4 807,004 - Sama_0651 0.41 -2.6 807,069 + Sama_0651 0.46 +0.3 807,069 + Sama_0651 0.46 +1.2 807,069 + Sama_0651 0.46 +1.6 807,070 - Sama_0651 0.47 -2.2 807,070 - Sama_0651 0.47 -0.9 807,070 - Sama_0651 0.47 -1.7 807,070 - Sama_0651 0.47 -0.6 807,070 - Sama_0651 0.47 +0.3 807,073 + Sama_0651 0.47 -0.4 807,073 + Sama_0651 0.47 -1.2 807,073 + Sama_0651 0.47 +1.6 807,073 + Sama_0651 0.47 -1.6 807,074 - Sama_0651 0.47 -1.4 807,136 - Sama_0651 0.52 +0.6 807,141 + Sama_0651 0.52 +1.1 807,223 - Sama_0651 0.59 -1.4 807,237 + Sama_0651 0.60 +1.3 807,311 + Sama_0651 0.66 +0.7 807,311 + Sama_0651 0.66 -1.3 807,311 + Sama_0651 0.66 +0.7 807,432 + Sama_0651 0.75 +0.8 807,433 - Sama_0651 0.76 +0.6 807,433 - Sama_0651 0.76 +0.1 807,433 - Sama_0651 0.76 -0.3 807,471 - Sama_0651 0.79 +0.7 807,471 - Sama_0651 0.79 +0.6
Or see this region's nucleotide sequence