Experiment: a-Ketoglutaric
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Sama_0014 and recF are separated by 27 nucleotides recF and Sama_0016 are separated by 92 nucleotides
Sama_0014: Sama_0014 - DNA-directed DNA polymerase (RefSeq), at 12,168 to 13,268
_0014
Sama_0015: recF - recombination protein F (RefSeq), at 13,296 to 14,378
recF
Sama_0016: Sama_0016 - DNA topoisomerase (ATP-hydrolyzing) (RefSeq), at 14,471 to 16,888
_0016
Position (kb) 13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 13.266 kb on + strand at 13.266 kb on + strand at 13.266 kb on + strand at 13.266 kb on + strand at 13.267 kb on - strand at 13.295 kb on + strand at 13.295 kb on + strand at 13.296 kb on - strand at 13.296 kb on - strand at 13.296 kb on - strand at 13.334 kb on + strand at 13.335 kb on - strand at 13.335 kb on - strand at 13.380 kb on + strand at 13.380 kb on + strand at 13.381 kb on - strand at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.425 kb on + strand, within recF at 13.452 kb on - strand, within recF at 13.452 kb on - strand, within recF at 13.541 kb on + strand, within recF at 13.542 kb on - strand, within recF at 13.542 kb on - strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.630 kb on + strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.631 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.635 kb on - strand, within recF at 13.647 kb on - strand, within recF at 13.701 kb on - strand, within recF at 13.701 kb on - strand, within recF at 13.754 kb on + strand, within recF at 13.754 kb on + strand, within recF at 13.755 kb on - strand, within recF at 13.838 kb on - strand, within recF at 13.841 kb on + strand, within recF at 13.867 kb on + strand, within recF at 13.867 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.878 kb on + strand, within recF at 13.879 kb on - strand, within recF at 13.879 kb on - strand, within recF at 13.879 kb on - strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.882 kb on + strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.883 kb on - strand, within recF at 13.904 kb on + strand, within recF at 13.904 kb on + strand, within recF at 13.904 kb on + strand, within recF at 13.910 kb on + strand, within recF at 13.910 kb on + strand, within recF at 13.912 kb on + strand, within recF at 13.912 kb on + strand, within recF at 14.007 kb on + strand, within recF at 14.026 kb on + strand, within recF at 14.033 kb on + strand, within recF at 14.033 kb on + strand, within recF at 14.051 kb on + strand, within recF at 14.051 kb on + strand, within recF at 14.078 kb on + strand, within recF at 14.078 kb on + strand, within recF at 14.122 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.162 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.190 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.257 kb on + strand, within recF at 14.261 kb on + strand, within recF at 14.261 kb on + strand, within recF at 14.347 kb on + strand at 14.347 kb on + strand at 14.347 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.372 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand at 14.376 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric remove 13,266 + +0.2 13,266 + -0.5 13,266 + +0.5 13,266 + -0.9 13,267 - +1.0 13,295 + -0.4 13,295 + +1.7 13,296 - -1.5 13,296 - +1.1 13,296 - -1.5 13,334 + -1.0 13,335 - -1.2 13,335 - +0.9 13,380 + -2.8 13,380 + +0.6 13,381 - -2.9 13,425 + recF Sama_0015 0.12 -2.0 13,425 + recF Sama_0015 0.12 -1.1 13,425 + recF Sama_0015 0.12 -1.7 13,425 + recF Sama_0015 0.12 -3.3 13,425 + recF Sama_0015 0.12 -1.7 13,452 - recF Sama_0015 0.14 +0.8 13,452 - recF Sama_0015 0.14 +0.6 13,541 + recF Sama_0015 0.23 -1.5 13,542 - recF Sama_0015 0.23 -0.1 13,542 - recF Sama_0015 0.23 -1.3 13,630 + recF Sama_0015 0.31 +1.2 13,630 + recF Sama_0015 0.31 +0.3 13,630 + recF Sama_0015 0.31 -1.3 13,630 + recF Sama_0015 0.31 +1.3 13,630 + recF Sama_0015 0.31 -0.5 13,630 + recF Sama_0015 0.31 -1.9 13,630 + recF Sama_0015 0.31 -0.2 13,630 + recF Sama_0015 0.31 -0.9 13,631 - recF Sama_0015 0.31 +0.6 13,631 - recF Sama_0015 0.31 -0.2 13,631 - recF Sama_0015 0.31 +1.1 13,631 - recF Sama_0015 0.31 -1.7 13,635 - recF Sama_0015 0.31 +1.2 13,635 - recF Sama_0015 0.31 -2.0 13,635 - recF Sama_0015 0.31 -2.4 13,635 - recF Sama_0015 0.31 -1.3 13,647 - recF Sama_0015 0.32 -2.0 13,701 - recF Sama_0015 0.37 +1.1 13,701 - recF Sama_0015 0.37 -2.7 13,754 + recF Sama_0015 0.42 -2.2 13,754 + recF Sama_0015 0.42 -2.4 13,755 - recF Sama_0015 0.42 +0.2 13,838 - recF Sama_0015 0.50 +2.9 13,841 + recF Sama_0015 0.50 -0.7 13,867 + recF Sama_0015 0.53 +0.1 13,867 + recF Sama_0015 0.53 -1.7 13,878 + recF Sama_0015 0.54 -2.2 13,878 + recF Sama_0015 0.54 -2.2 13,878 + recF Sama_0015 0.54 +0.4 13,878 + recF Sama_0015 0.54 -1.1 13,878 + recF Sama_0015 0.54 -3.6 13,879 - recF Sama_0015 0.54 -0.9 13,879 - recF Sama_0015 0.54 -3.1 13,879 - recF Sama_0015 0.54 -3.2 13,882 + recF Sama_0015 0.54 -0.5 13,882 + recF Sama_0015 0.54 -1.1 13,882 + recF Sama_0015 0.54 -0.3 13,882 + recF Sama_0015 0.54 -3.1 13,883 - recF Sama_0015 0.54 -0.5 13,883 - recF Sama_0015 0.54 -1.9 13,883 - recF Sama_0015 0.54 +2.0 13,883 - recF Sama_0015 0.54 -1.9 13,883 - recF Sama_0015 0.54 +1.9 13,883 - recF Sama_0015 0.54 +1.5 13,904 + recF Sama_0015 0.56 -1.4 13,904 + recF Sama_0015 0.56 -2.2 13,904 + recF Sama_0015 0.56 +2.9 13,910 + recF Sama_0015 0.57 +1.0 13,910 + recF Sama_0015 0.57 -0.1 13,912 + recF Sama_0015 0.57 +0.5 13,912 + recF Sama_0015 0.57 +0.6 14,007 + recF Sama_0015 0.66 -0.2 14,026 + recF Sama_0015 0.67 -1.3 14,033 + recF Sama_0015 0.68 -0.5 14,033 + recF Sama_0015 0.68 -1.7 14,051 + recF Sama_0015 0.70 -1.7 14,051 + recF Sama_0015 0.70 +0.9 14,078 + recF Sama_0015 0.72 -1.1 14,078 + recF Sama_0015 0.72 +2.1 14,122 + recF Sama_0015 0.76 +0.0 14,162 + recF Sama_0015 0.80 +0.5 14,162 + recF Sama_0015 0.80 -0.5 14,162 + recF Sama_0015 0.80 -3.0 14,190 + recF Sama_0015 0.83 -0.5 14,190 + recF Sama_0015 0.83 -1.5 14,190 + recF Sama_0015 0.83 -3.3 14,190 + recF Sama_0015 0.83 +0.1 14,190 + recF Sama_0015 0.83 -1.7 14,257 + recF Sama_0015 0.89 -1.1 14,257 + recF Sama_0015 0.89 -0.5 14,257 + recF Sama_0015 0.89 -1.9 14,261 + recF Sama_0015 0.89 -1.9 14,261 + recF Sama_0015 0.89 -2.1 14,347 + -0.8 14,347 + +0.5 14,347 + +1.1 14,372 + -0.1 14,372 + -0.1 14,372 + -0.4 14,372 + +1.6 14,376 + +1.0 14,376 + -0.3 14,376 + +1.5 14,376 + +0.7
Or see this region's nucleotide sequence