Experiment: m.b. Polymyxin B sulfate 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00780 and PGA1_c00790 are separated by 58 nucleotides PGA1_c00790 and PGA1_c00800 are separated by 63 nucleotides PGA1_c00800 and PGA1_c00810 are separated by 40 nucleotides
PGA1_c00780: PGA1_c00780 - putative chromosome segregation protein SMC, at 75,090 to 78,545
_c00780
PGA1_c00790: PGA1_c00790 - hypothetical protein, at 78,604 to 79,071
_c00790
PGA1_c00800: PGA1_c00800 - hypothetical protein, at 79,135 to 79,524
_c00800
PGA1_c00810: PGA1_c00810 - putative lytic murein transglycosylase, at 79,565 to 80,737
_c00810
Position (kb)
78
79
80 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 77.635 kb on - strand, within PGA1_c00780 at 77.635 kb on - strand, within PGA1_c00780 at 77.641 kb on + strand, within PGA1_c00780 at 77.678 kb on - strand, within PGA1_c00780 at 77.751 kb on - strand, within PGA1_c00780 at 77.751 kb on - strand, within PGA1_c00780 at 77.775 kb on + strand, within PGA1_c00780 at 77.808 kb on + strand, within PGA1_c00780 at 77.813 kb on + strand, within PGA1_c00780 at 77.816 kb on - strand, within PGA1_c00780 at 77.816 kb on - strand, within PGA1_c00780 at 77.840 kb on - strand, within PGA1_c00780 at 77.850 kb on - strand, within PGA1_c00780 at 77.856 kb on + strand, within PGA1_c00780 at 77.864 kb on - strand, within PGA1_c00780 at 77.902 kb on - strand, within PGA1_c00780 at 77.940 kb on - strand, within PGA1_c00780 at 78.074 kb on + strand, within PGA1_c00780 at 78.085 kb on + strand, within PGA1_c00780 at 78.093 kb on - strand, within PGA1_c00780 at 78.109 kb on + strand, within PGA1_c00780 at 78.151 kb on + strand, within PGA1_c00780 at 78.276 kb on + strand at 78.284 kb on - strand at 78.309 kb on + strand at 78.506 kb on - strand at 78.541 kb on + strand at 78.568 kb on + strand at 78.637 kb on - strand at 78.642 kb on - strand at 78.653 kb on + strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.661 kb on - strand, within PGA1_c00790 at 78.678 kb on + strand, within PGA1_c00790 at 78.740 kb on + strand, within PGA1_c00790 at 78.772 kb on + strand, within PGA1_c00790 at 78.803 kb on + strand, within PGA1_c00790 at 78.804 kb on + strand, within PGA1_c00790 at 78.811 kb on - strand, within PGA1_c00790 at 78.859 kb on - strand, within PGA1_c00790 at 78.935 kb on - strand, within PGA1_c00790 at 79.037 kb on - strand at 79.065 kb on + strand at 79.075 kb on + strand at 79.075 kb on + strand at 79.075 kb on + strand at 79.083 kb on - strand at 79.083 kb on - strand at 79.133 kb on + strand at 79.186 kb on + strand, within PGA1_c00800 at 79.209 kb on + strand, within PGA1_c00800 at 79.227 kb on - strand, within PGA1_c00800 at 79.244 kb on - strand, within PGA1_c00800 at 79.308 kb on + strand, within PGA1_c00800 at 79.366 kb on + strand, within PGA1_c00800 at 79.374 kb on - strand, within PGA1_c00800 at 79.374 kb on - strand, within PGA1_c00800 at 79.382 kb on - strand, within PGA1_c00800 at 79.428 kb on + strand, within PGA1_c00800 at 79.458 kb on + strand, within PGA1_c00800 at 79.518 kb on + strand at 79.526 kb on - strand at 79.539 kb on + strand at 79.561 kb on - strand at 79.580 kb on - strand at 79.580 kb on - strand at 79.580 kb on - strand at 79.587 kb on + strand at 79.659 kb on + strand at 79.684 kb on + strand, within PGA1_c00810 at 79.684 kb on + strand, within PGA1_c00810 at 79.684 kb on + strand, within PGA1_c00810 at 79.684 kb on + strand, within PGA1_c00810 at 79.761 kb on + strand, within PGA1_c00810 at 79.813 kb on + strand, within PGA1_c00810 at 79.813 kb on + strand, within PGA1_c00810 at 79.821 kb on - strand, within PGA1_c00810 at 79.826 kb on + strand, within PGA1_c00810 at 79.898 kb on - strand, within PGA1_c00810 at 79.988 kb on + strand, within PGA1_c00810
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0008 mg/ml remove 77,635 - PGA1_c00780 0.74 -2.0 77,635 - PGA1_c00780 0.74 -2.7 77,641 + PGA1_c00780 0.74 -1.9 77,678 - PGA1_c00780 0.75 +0.2 77,751 - PGA1_c00780 0.77 -2.5 77,751 - PGA1_c00780 0.77 -0.1 77,775 + PGA1_c00780 0.78 -2.1 77,808 + PGA1_c00780 0.79 -0.9 77,813 + PGA1_c00780 0.79 +0.7 77,816 - PGA1_c00780 0.79 -1.7 77,816 - PGA1_c00780 0.79 +0.5 77,840 - PGA1_c00780 0.80 -0.5 77,850 - PGA1_c00780 0.80 -2.5 77,856 + PGA1_c00780 0.80 -0.1 77,864 - PGA1_c00780 0.80 -0.7 77,902 - PGA1_c00780 0.81 -1.7 77,940 - PGA1_c00780 0.82 -0.7 78,074 + PGA1_c00780 0.86 -1.7 78,085 + PGA1_c00780 0.87 -3.0 78,093 - PGA1_c00780 0.87 -0.1 78,109 + PGA1_c00780 0.87 -0.5 78,151 + PGA1_c00780 0.89 -1.1 78,276 + -1.3 78,284 - -0.0 78,309 + -0.7 78,506 - -1.3 78,541 + +0.5 78,568 + -0.4 78,637 - +1.8 78,642 - +0.1 78,653 + PGA1_c00790 0.10 +0.3 78,661 - PGA1_c00790 0.12 -0.1 78,661 - PGA1_c00790 0.12 +0.8 78,661 - PGA1_c00790 0.12 -2.1 78,678 + PGA1_c00790 0.16 -0.3 78,740 + PGA1_c00790 0.29 -0.4 78,772 + PGA1_c00790 0.36 -1.3 78,803 + PGA1_c00790 0.43 -0.9 78,804 + PGA1_c00790 0.43 -1.3 78,811 - PGA1_c00790 0.44 -0.7 78,859 - PGA1_c00790 0.54 -1.9 78,935 - PGA1_c00790 0.71 -1.7 79,037 - -1.3 79,065 + -0.3 79,075 + +1.4 79,075 + -0.5 79,075 + +0.1 79,083 - -2.3 79,083 - -1.5 79,133 + +0.1 79,186 + PGA1_c00800 0.13 -2.1 79,209 + PGA1_c00800 0.19 -1.0 79,227 - PGA1_c00800 0.24 -0.3 79,244 - PGA1_c00800 0.28 +0.9 79,308 + PGA1_c00800 0.44 -0.8 79,366 + PGA1_c00800 0.59 -2.5 79,374 - PGA1_c00800 0.61 -0.1 79,374 - PGA1_c00800 0.61 +0.7 79,382 - PGA1_c00800 0.63 +1.0 79,428 + PGA1_c00800 0.75 +1.1 79,458 + PGA1_c00800 0.83 +0.7 79,518 + +1.3 79,526 - +1.3 79,539 + +0.2 79,561 - +2.6 79,580 - -0.8 79,580 - +0.1 79,580 - +1.3 79,587 + +0.6 79,659 + -0.2 79,684 + PGA1_c00810 0.10 -1.0 79,684 + PGA1_c00810 0.10 -0.7 79,684 + PGA1_c00810 0.10 -2.0 79,684 + PGA1_c00810 0.10 -2.3 79,761 + PGA1_c00810 0.17 -1.7 79,813 + PGA1_c00810 0.21 -0.7 79,813 + PGA1_c00810 0.21 -1.4 79,821 - PGA1_c00810 0.22 -0.1 79,826 + PGA1_c00810 0.22 -0.1 79,898 - PGA1_c00810 0.28 +0.1 79,988 + PGA1_c00810 0.36 -2.6
Or see this region's nucleotide sequence