Experiment: m.b. Polymyxin B sulfate 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c05290 and PGA1_c05300 are separated by 144 nucleotides PGA1_c05300 and PGA1_c05310 are separated by 18 nucleotides PGA1_c05310 and PGA1_c05320 overlap by 4 nucleotides
PGA1_c05290: PGA1_c05290 - polysaccharide biosynthesis protein ExoT-like protein, at 524,993 to 526,357
_c05290
PGA1_c05300: PGA1_c05300 - putative glycosyl transferase, at 526,502 to 527,719
_c05300
PGA1_c05310: PGA1_c05310 - putative glycosyl transferase, at 527,738 to 528,580
_c05310
PGA1_c05320: PGA1_c05320 - hypothetical protein, at 528,577 to 531,147
_c05320
Position (kb)
526
527
528 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 525.503 kb on - strand, within PGA1_c05290 at 525.509 kb on - strand, within PGA1_c05290 at 525.541 kb on + strand, within PGA1_c05290 at 525.548 kb on + strand, within PGA1_c05290 at 525.549 kb on - strand, within PGA1_c05290 at 525.556 kb on - strand, within PGA1_c05290 at 525.596 kb on + strand, within PGA1_c05290 at 525.613 kb on + strand, within PGA1_c05290 at 525.626 kb on - strand, within PGA1_c05290 at 525.628 kb on + strand, within PGA1_c05290 at 525.674 kb on - strand, within PGA1_c05290 at 525.712 kb on - strand, within PGA1_c05290 at 525.754 kb on + strand, within PGA1_c05290 at 525.762 kb on - strand, within PGA1_c05290 at 525.796 kb on - strand, within PGA1_c05290 at 525.798 kb on - strand, within PGA1_c05290 at 525.836 kb on + strand, within PGA1_c05290 at 525.844 kb on - strand, within PGA1_c05290 at 525.888 kb on - strand, within PGA1_c05290 at 525.924 kb on + strand, within PGA1_c05290 at 525.940 kb on + strand, within PGA1_c05290 at 525.943 kb on - strand, within PGA1_c05290 at 525.953 kb on - strand, within PGA1_c05290 at 525.984 kb on + strand, within PGA1_c05290 at 525.984 kb on + strand, within PGA1_c05290 at 525.986 kb on + strand, within PGA1_c05290 at 525.992 kb on - strand, within PGA1_c05290 at 525.992 kb on - strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.029 kb on + strand, within PGA1_c05290 at 526.036 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.037 kb on - strand, within PGA1_c05290 at 526.062 kb on - strand, within PGA1_c05290 at 526.080 kb on - strand, within PGA1_c05290 at 526.116 kb on + strand, within PGA1_c05290 at 526.116 kb on + strand, within PGA1_c05290 at 526.182 kb on + strand, within PGA1_c05290 at 526.185 kb on - strand, within PGA1_c05290 at 526.187 kb on + strand, within PGA1_c05290 at 526.190 kb on - strand, within PGA1_c05290 at 526.285 kb on - strand at 526.304 kb on - strand at 526.330 kb on - strand at 526.346 kb on + strand at 526.390 kb on + strand at 526.437 kb on - strand at 526.532 kb on - strand at 526.535 kb on - strand at 526.573 kb on - strand at 526.579 kb on - strand at 526.593 kb on - strand at 526.660 kb on + strand, within PGA1_c05300 at 526.660 kb on + strand, within PGA1_c05300 at 526.663 kb on - strand, within PGA1_c05300 at 526.663 kb on - strand, within PGA1_c05300 at 526.668 kb on + strand, within PGA1_c05300 at 526.671 kb on + strand, within PGA1_c05300 at 526.752 kb on - strand, within PGA1_c05300 at 526.759 kb on + strand, within PGA1_c05300 at 526.925 kb on + strand, within PGA1_c05300 at 526.932 kb on - strand, within PGA1_c05300 at 526.939 kb on + strand, within PGA1_c05300 at 526.988 kb on - strand, within PGA1_c05300 at 526.988 kb on - strand, within PGA1_c05300 at 527.083 kb on - strand, within PGA1_c05300 at 527.085 kb on + strand, within PGA1_c05300 at 527.093 kb on + strand, within PGA1_c05300 at 527.115 kb on + strand, within PGA1_c05300 at 527.124 kb on - strand, within PGA1_c05300 at 527.154 kb on - strand, within PGA1_c05300 at 527.226 kb on - strand, within PGA1_c05300 at 527.250 kb on + strand, within PGA1_c05300 at 527.250 kb on + strand, within PGA1_c05300 at 527.258 kb on - strand, within PGA1_c05300 at 527.277 kb on + strand, within PGA1_c05300 at 527.320 kb on + strand, within PGA1_c05300 at 527.371 kb on + strand, within PGA1_c05300 at 527.376 kb on + strand, within PGA1_c05300 at 527.379 kb on - strand, within PGA1_c05300 at 527.379 kb on - strand, within PGA1_c05300 at 527.384 kb on - strand, within PGA1_c05300 at 527.534 kb on - strand, within PGA1_c05300 at 527.578 kb on - strand, within PGA1_c05300 at 527.590 kb on + strand, within PGA1_c05300 at 527.618 kb on - strand at 527.636 kb on + strand at 527.686 kb on + strand at 527.729 kb on + strand at 527.731 kb on + strand at 527.827 kb on - strand, within PGA1_c05310 at 527.850 kb on - strand, within PGA1_c05310 at 527.862 kb on - strand, within PGA1_c05310 at 527.866 kb on + strand, within PGA1_c05310 at 527.876 kb on + strand, within PGA1_c05310 at 527.891 kb on + strand, within PGA1_c05310 at 527.953 kb on + strand, within PGA1_c05310 at 528.030 kb on + strand, within PGA1_c05310 at 528.085 kb on - strand, within PGA1_c05310 at 528.096 kb on + strand, within PGA1_c05310 at 528.122 kb on + strand, within PGA1_c05310 at 528.132 kb on - strand, within PGA1_c05310 at 528.132 kb on - strand, within PGA1_c05310 at 528.146 kb on + strand, within PGA1_c05310 at 528.156 kb on + strand, within PGA1_c05310 at 528.159 kb on - strand, within PGA1_c05310 at 528.164 kb on - strand, within PGA1_c05310 at 528.172 kb on + strand, within PGA1_c05310 at 528.177 kb on - strand, within PGA1_c05310 at 528.198 kb on + strand, within PGA1_c05310 at 528.240 kb on + strand, within PGA1_c05310 at 528.240 kb on - strand, within PGA1_c05310 at 528.252 kb on + strand, within PGA1_c05310 at 528.252 kb on + strand, within PGA1_c05310 at 528.257 kb on + strand, within PGA1_c05310 at 528.260 kb on - strand, within PGA1_c05310 at 528.260 kb on - strand, within PGA1_c05310 at 528.260 kb on - strand, within PGA1_c05310 at 528.313 kb on - strand, within PGA1_c05310 at 528.313 kb on - strand, within PGA1_c05310 at 528.363 kb on + strand, within PGA1_c05310 at 528.363 kb on + strand, within PGA1_c05310 at 528.363 kb on + strand, within PGA1_c05310 at 528.363 kb on + strand, within PGA1_c05310 at 528.371 kb on - strand, within PGA1_c05310 at 528.371 kb on - strand, within PGA1_c05310 at 528.371 kb on - strand, within PGA1_c05310 at 528.382 kb on + strand, within PGA1_c05310 at 528.390 kb on + strand, within PGA1_c05310 at 528.424 kb on + strand, within PGA1_c05310 at 528.441 kb on + strand, within PGA1_c05310 at 528.522 kb on + strand at 528.545 kb on - strand at 528.637 kb on + strand at 528.637 kb on + strand at 528.669 kb on - strand at 528.688 kb on - strand at 528.692 kb on + strand at 528.702 kb on + strand at 528.710 kb on - strand at 528.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0008 mg/ml remove 525,503 - PGA1_c05290 0.37 +0.7 525,509 - PGA1_c05290 0.38 +0.3 525,541 + PGA1_c05290 0.40 +2.2 525,548 + PGA1_c05290 0.41 +0.5 525,549 - PGA1_c05290 0.41 -0.6 525,556 - PGA1_c05290 0.41 +1.1 525,596 + PGA1_c05290 0.44 +1.0 525,613 + PGA1_c05290 0.45 -0.6 525,626 - PGA1_c05290 0.46 +0.7 525,628 + PGA1_c05290 0.47 +1.6 525,674 - PGA1_c05290 0.50 +1.4 525,712 - PGA1_c05290 0.53 +0.0 525,754 + PGA1_c05290 0.56 +0.7 525,762 - PGA1_c05290 0.56 +0.8 525,796 - PGA1_c05290 0.59 +0.7 525,798 - PGA1_c05290 0.59 +2.2 525,836 + PGA1_c05290 0.62 -0.4 525,844 - PGA1_c05290 0.62 -0.2 525,888 - PGA1_c05290 0.66 +0.4 525,924 + PGA1_c05290 0.68 +0.9 525,940 + PGA1_c05290 0.69 +0.7 525,943 - PGA1_c05290 0.70 +0.6 525,953 - PGA1_c05290 0.70 -0.1 525,984 + PGA1_c05290 0.73 -0.7 525,984 + PGA1_c05290 0.73 -0.1 525,986 + PGA1_c05290 0.73 +1.4 525,992 - PGA1_c05290 0.73 +0.4 525,992 - PGA1_c05290 0.73 +1.4 526,029 + PGA1_c05290 0.76 +1.0 526,029 + PGA1_c05290 0.76 +0.8 526,029 + PGA1_c05290 0.76 +1.0 526,036 - PGA1_c05290 0.76 +1.1 526,037 - PGA1_c05290 0.76 +1.3 526,037 - PGA1_c05290 0.76 +0.5 526,037 - PGA1_c05290 0.76 -0.9 526,062 - PGA1_c05290 0.78 +0.5 526,080 - PGA1_c05290 0.80 +0.3 526,116 + PGA1_c05290 0.82 +1.5 526,116 + PGA1_c05290 0.82 +0.9 526,182 + PGA1_c05290 0.87 -0.1 526,185 - PGA1_c05290 0.87 +0.3 526,187 + PGA1_c05290 0.87 +0.5 526,190 - PGA1_c05290 0.88 +1.0 526,285 - +1.2 526,304 - -1.4 526,330 - -0.0 526,346 + +1.3 526,390 + +1.2 526,437 - +0.7 526,532 - +0.5 526,535 - -0.4 526,573 - +0.9 526,579 - +1.6 526,593 - +0.7 526,660 + PGA1_c05300 0.13 +0.2 526,660 + PGA1_c05300 0.13 +1.0 526,663 - PGA1_c05300 0.13 -0.8 526,663 - PGA1_c05300 0.13 +0.3 526,668 + PGA1_c05300 0.14 +2.3 526,671 + PGA1_c05300 0.14 +0.4 526,752 - PGA1_c05300 0.21 +0.8 526,759 + PGA1_c05300 0.21 -0.2 526,925 + PGA1_c05300 0.35 +0.6 526,932 - PGA1_c05300 0.35 +1.7 526,939 + PGA1_c05300 0.36 -0.3 526,988 - PGA1_c05300 0.40 +0.7 526,988 - PGA1_c05300 0.40 +0.6 527,083 - PGA1_c05300 0.48 -0.3 527,085 + PGA1_c05300 0.48 +0.4 527,093 + PGA1_c05300 0.49 -0.4 527,115 + PGA1_c05300 0.50 +0.9 527,124 - PGA1_c05300 0.51 -0.2 527,154 - PGA1_c05300 0.54 +0.6 527,226 - PGA1_c05300 0.59 -0.9 527,250 + PGA1_c05300 0.61 +0.7 527,250 + PGA1_c05300 0.61 +0.4 527,258 - PGA1_c05300 0.62 +1.1 527,277 + PGA1_c05300 0.64 -0.0 527,320 + PGA1_c05300 0.67 +0.1 527,371 + PGA1_c05300 0.71 +0.8 527,376 + PGA1_c05300 0.72 +0.6 527,379 - PGA1_c05300 0.72 -0.0 527,379 - PGA1_c05300 0.72 -0.5 527,384 - PGA1_c05300 0.72 +0.1 527,534 - PGA1_c05300 0.85 +0.7 527,578 - PGA1_c05300 0.88 +0.3 527,590 + PGA1_c05300 0.89 +0.7 527,618 - +0.2 527,636 + +0.8 527,686 + -0.4 527,729 + -0.3 527,731 + +0.1 527,827 - PGA1_c05310 0.11 +0.6 527,850 - PGA1_c05310 0.13 -1.0 527,862 - PGA1_c05310 0.15 +0.7 527,866 + PGA1_c05310 0.15 +0.9 527,876 + PGA1_c05310 0.16 +0.5 527,891 + PGA1_c05310 0.18 +1.2 527,953 + PGA1_c05310 0.26 -0.2 528,030 + PGA1_c05310 0.35 +0.6 528,085 - PGA1_c05310 0.41 +0.3 528,096 + PGA1_c05310 0.42 -0.6 528,122 + PGA1_c05310 0.46 +0.3 528,132 - PGA1_c05310 0.47 +0.9 528,132 - PGA1_c05310 0.47 +0.5 528,146 + PGA1_c05310 0.48 +0.8 528,156 + PGA1_c05310 0.50 +0.5 528,159 - PGA1_c05310 0.50 -0.4 528,164 - PGA1_c05310 0.51 -1.2 528,172 + PGA1_c05310 0.51 -1.5 528,177 - PGA1_c05310 0.52 -0.3 528,198 + PGA1_c05310 0.55 -0.7 528,240 + PGA1_c05310 0.60 -0.3 528,240 - PGA1_c05310 0.60 +0.2 528,252 + PGA1_c05310 0.61 -0.3 528,252 + PGA1_c05310 0.61 -0.1 528,257 + PGA1_c05310 0.62 -1.2 528,260 - PGA1_c05310 0.62 +0.6 528,260 - PGA1_c05310 0.62 +0.1 528,260 - PGA1_c05310 0.62 -0.4 528,313 - PGA1_c05310 0.68 -0.5 528,313 - PGA1_c05310 0.68 -0.9 528,363 + PGA1_c05310 0.74 +1.0 528,363 + PGA1_c05310 0.74 -0.4 528,363 + PGA1_c05310 0.74 +0.5 528,363 + PGA1_c05310 0.74 +1.4 528,371 - PGA1_c05310 0.75 -1.4 528,371 - PGA1_c05310 0.75 +1.0 528,371 - PGA1_c05310 0.75 +0.2 528,382 + PGA1_c05310 0.76 -0.8 528,390 + PGA1_c05310 0.77 +0.6 528,424 + PGA1_c05310 0.81 +0.7 528,441 + PGA1_c05310 0.83 +0.2 528,522 + -2.6 528,545 - +0.7 528,637 + -2.2 528,637 + +1.4 528,669 - -0.0 528,688 - +0.5 528,692 + +1.6 528,702 + +1.3 528,710 - +1.0 528,710 - +0.2
Or see this region's nucleotide sequence