Experiment: m.b. Polymyxin B sulfate 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20650 and PGA1_c20660 are separated by 200 nucleotides PGA1_c20660 and PGA1_c20670 overlap by 1 nucleotides PGA1_c20670 and PGA1_c20680 are separated by 108 nucleotides
PGA1_c20650: PGA1_c20650 - triosephosphate isomerase TpiA, at 2,140,103 to 2,140,855
_c20650
PGA1_c20660: PGA1_c20660 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxogulatarate, large permease component (from data) , at 2,141,056 to 2,142,459
_c20660
PGA1_c20670: PGA1_c20670 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxogulatarate, small permease component (from data) , at 2,142,459 to 2,143,136
_c20670
PGA1_c20680: PGA1_c20680 - TRAP transporter for fumarate, succinate, L-malate, and 2-oxogulatarate, substrate-binding component (from data) , at 2,143,245 to 2,144,246
_c20680
Position (kb)
2141
2142
2143 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2140.061 kb on + strand at 2140.069 kb on - strand at 2140.092 kb on - strand at 2140.863 kb on + strand at 2140.915 kb on - strand at 2141.102 kb on + strand at 2141.102 kb on + strand at 2141.115 kb on + strand at 2141.115 kb on + strand at 2141.154 kb on - strand at 2141.213 kb on - strand, within PGA1_c20660 at 2141.224 kb on + strand, within PGA1_c20660 at 2141.231 kb on - strand, within PGA1_c20660 at 2141.286 kb on + strand, within PGA1_c20660 at 2141.429 kb on - strand, within PGA1_c20660 at 2141.586 kb on + strand, within PGA1_c20660 at 2141.721 kb on - strand, within PGA1_c20660 at 2141.863 kb on - strand, within PGA1_c20660 at 2141.886 kb on + strand, within PGA1_c20660 at 2142.045 kb on - strand, within PGA1_c20660 at 2142.045 kb on - strand, within PGA1_c20660 at 2142.150 kb on + strand, within PGA1_c20660 at 2142.382 kb on - strand at 2142.418 kb on + strand at 2142.450 kb on - strand at 2142.462 kb on + strand at 2142.608 kb on + strand, within PGA1_c20670 at 2142.613 kb on - strand, within PGA1_c20670 at 2142.630 kb on - strand, within PGA1_c20670 at 2142.665 kb on - strand, within PGA1_c20670 at 2142.694 kb on + strand, within PGA1_c20670 at 2142.699 kb on + strand, within PGA1_c20670 at 2142.707 kb on - strand, within PGA1_c20670 at 2142.745 kb on - strand, within PGA1_c20670 at 2142.793 kb on + strand, within PGA1_c20670 at 2142.808 kb on - strand, within PGA1_c20670 at 2142.839 kb on + strand, within PGA1_c20670 at 2142.847 kb on - strand, within PGA1_c20670 at 2142.902 kb on - strand, within PGA1_c20670 at 2142.912 kb on + strand, within PGA1_c20670 at 2142.937 kb on - strand, within PGA1_c20670 at 2143.104 kb on - strand at 2143.120 kb on + strand at 2143.121 kb on + strand at 2143.153 kb on - strand at 2143.352 kb on + strand, within PGA1_c20680 at 2143.360 kb on - strand, within PGA1_c20680 at 2143.360 kb on - strand, within PGA1_c20680 at 2143.411 kb on + strand, within PGA1_c20680 at 2143.411 kb on + strand, within PGA1_c20680 at 2143.419 kb on - strand, within PGA1_c20680 at 2143.419 kb on - strand, within PGA1_c20680 at 2143.419 kb on - strand, within PGA1_c20680
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0008 mg/ml remove 2,140,061 + +1.8 2,140,069 - -0.3 2,140,092 - -0.5 2,140,863 + -0.4 2,140,915 - +0.1 2,141,102 + -0.5 2,141,102 + +0.3 2,141,115 + +0.5 2,141,115 + -0.2 2,141,154 - +0.1 2,141,213 - PGA1_c20660 0.11 -1.2 2,141,224 + PGA1_c20660 0.12 -1.1 2,141,231 - PGA1_c20660 0.12 +1.8 2,141,286 + PGA1_c20660 0.16 -1.0 2,141,429 - PGA1_c20660 0.27 -1.0 2,141,586 + PGA1_c20660 0.38 +2.3 2,141,721 - PGA1_c20660 0.47 -0.3 2,141,863 - PGA1_c20660 0.57 +0.1 2,141,886 + PGA1_c20660 0.59 -0.7 2,142,045 - PGA1_c20660 0.70 -0.1 2,142,045 - PGA1_c20660 0.70 -1.1 2,142,150 + PGA1_c20660 0.78 -0.2 2,142,382 - -1.0 2,142,418 + -1.3 2,142,450 - +0.2 2,142,462 + -0.0 2,142,608 + PGA1_c20670 0.22 +1.5 2,142,613 - PGA1_c20670 0.23 +0.7 2,142,630 - PGA1_c20670 0.25 +0.1 2,142,665 - PGA1_c20670 0.30 -1.4 2,142,694 + PGA1_c20670 0.35 -0.5 2,142,699 + PGA1_c20670 0.35 -1.5 2,142,707 - PGA1_c20670 0.37 +0.5 2,142,745 - PGA1_c20670 0.42 -2.7 2,142,793 + PGA1_c20670 0.49 +0.7 2,142,808 - PGA1_c20670 0.51 -1.5 2,142,839 + PGA1_c20670 0.56 -0.5 2,142,847 - PGA1_c20670 0.57 -0.1 2,142,902 - PGA1_c20670 0.65 -0.6 2,142,912 + PGA1_c20670 0.67 +0.0 2,142,937 - PGA1_c20670 0.71 -0.4 2,143,104 - -0.7 2,143,120 + -2.7 2,143,121 + +0.0 2,143,153 - -0.0 2,143,352 + PGA1_c20680 0.11 +2.5 2,143,360 - PGA1_c20680 0.11 +0.5 2,143,360 - PGA1_c20680 0.11 +0.7 2,143,411 + PGA1_c20680 0.17 -1.9 2,143,411 + PGA1_c20680 0.17 +0.7 2,143,419 - PGA1_c20680 0.17 -0.2 2,143,419 - PGA1_c20680 0.17 -0.3 2,143,419 - PGA1_c20680 0.17 +1.0
Or see this region's nucleotide sequence