Experiment: m.b. Polymyxin B sulfate 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c10280 and PGA1_c10290 are separated by 141 nucleotides PGA1_c10290 and PGA1_c10300 are separated by 164 nucleotides PGA1_c10300 and PGA1_c10310 are separated by 137 nucleotides PGA1_c10310 and PGA1_c10320 are separated by 17 nucleotides
PGA1_c10280: PGA1_c10280 - Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) (from data) , at 1,065,289 to 1,066,449
_c10280
PGA1_c10290: PGA1_c10290 - outer membrane protein, at 1,066,591 to 1,067,193
_c10290
PGA1_c10300: PGA1_c10300 - acetyl-coenzyme A synthetase AcsA, at 1,067,358 to 1,068,908
_c10300
PGA1_c10310: PGA1_c10310 - hypothetical protein, at 1,069,046 to 1,069,381
_c10310
PGA1_c10320: PGA1_c10320 - methylcrotonoyl-CoA carboxylase beta chain, at 1,069,399 to 1,070,982
_c10320
Position (kb)
1067
1068
1069 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1066.446 kb on - strand at 1066.531 kb on + strand at 1066.539 kb on - strand at 1066.614 kb on + strand at 1066.614 kb on + strand at 1066.614 kb on + strand at 1066.622 kb on - strand at 1066.622 kb on - strand at 1066.665 kb on + strand, within PGA1_c10290 at 1066.674 kb on - strand, within PGA1_c10290 at 1066.702 kb on + strand, within PGA1_c10290 at 1066.768 kb on + strand, within PGA1_c10290 at 1066.776 kb on - strand, within PGA1_c10290 at 1066.788 kb on - strand, within PGA1_c10290 at 1066.822 kb on + strand, within PGA1_c10290 at 1066.843 kb on + strand, within PGA1_c10290 at 1066.900 kb on + strand, within PGA1_c10290 at 1067.006 kb on + strand, within PGA1_c10290 at 1067.006 kb on - strand, within PGA1_c10290 at 1067.011 kb on + strand, within PGA1_c10290 at 1067.014 kb on - strand, within PGA1_c10290 at 1067.014 kb on - strand, within PGA1_c10290 at 1067.019 kb on - strand, within PGA1_c10290 at 1067.114 kb on + strand, within PGA1_c10290 at 1067.114 kb on + strand, within PGA1_c10290 at 1067.122 kb on - strand, within PGA1_c10290 at 1067.130 kb on + strand, within PGA1_c10290 at 1067.130 kb on + strand, within PGA1_c10290 at 1067.135 kb on + strand at 1067.138 kb on - strand at 1067.181 kb on + strand at 1067.205 kb on - strand at 1067.239 kb on + strand at 1067.247 kb on - strand at 1067.270 kb on - strand at 1067.272 kb on + strand at 1067.350 kb on + strand at 1067.404 kb on - strand at 1067.448 kb on + strand at 1067.770 kb on - strand, within PGA1_c10300 at 1067.831 kb on + strand, within PGA1_c10300 at 1067.832 kb on + strand, within PGA1_c10300 at 1067.876 kb on + strand, within PGA1_c10300 at 1067.884 kb on - strand, within PGA1_c10300 at 1067.996 kb on + strand, within PGA1_c10300 at 1068.073 kb on - strand, within PGA1_c10300 at 1068.129 kb on + strand, within PGA1_c10300 at 1068.231 kb on - strand, within PGA1_c10300 at 1068.267 kb on - strand, within PGA1_c10300 at 1068.268 kb on + strand, within PGA1_c10300 at 1068.276 kb on - strand, within PGA1_c10300 at 1068.327 kb on - strand, within PGA1_c10300 at 1068.394 kb on + strand, within PGA1_c10300 at 1068.475 kb on + strand, within PGA1_c10300 at 1068.597 kb on - strand, within PGA1_c10300 at 1068.605 kb on - strand, within PGA1_c10300 at 1068.722 kb on + strand, within PGA1_c10300 at 1068.730 kb on - strand, within PGA1_c10300 at 1068.730 kb on - strand, within PGA1_c10300 at 1068.810 kb on - strand at 1068.839 kb on + strand at 1068.845 kb on - strand at 1068.847 kb on - strand at 1068.954 kb on + strand at 1068.957 kb on - strand at 1068.964 kb on + strand at 1069.014 kb on - strand at 1069.035 kb on - strand at 1069.057 kb on + strand at 1069.057 kb on + strand at 1069.057 kb on - strand at 1069.088 kb on + strand, within PGA1_c10310 at 1069.114 kb on + strand, within PGA1_c10310 at 1069.119 kb on + strand, within PGA1_c10310 at 1069.119 kb on + strand, within PGA1_c10310 at 1069.122 kb on - strand, within PGA1_c10310 at 1069.122 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.127 kb on - strand, within PGA1_c10310 at 1069.129 kb on + strand, within PGA1_c10310 at 1069.152 kb on + strand, within PGA1_c10310 at 1069.152 kb on + strand, within PGA1_c10310 at 1069.160 kb on - strand, within PGA1_c10310 at 1069.160 kb on - strand, within PGA1_c10310 at 1069.175 kb on - strand, within PGA1_c10310 at 1069.253 kb on + strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.267 kb on - strand, within PGA1_c10310 at 1069.339 kb on + strand, within PGA1_c10310 at 1069.355 kb on + strand at 1069.361 kb on + strand at 1069.443 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Polymyxin B sulfate 0.0008 mg/ml remove 1,066,446 - -0.5 1,066,531 + -0.9 1,066,539 - +1.5 1,066,614 + -1.2 1,066,614 + -0.1 1,066,614 + +0.4 1,066,622 - -0.1 1,066,622 - +1.5 1,066,665 + PGA1_c10290 0.12 -1.1 1,066,674 - PGA1_c10290 0.14 -1.3 1,066,702 + PGA1_c10290 0.18 +0.4 1,066,768 + PGA1_c10290 0.29 +0.5 1,066,776 - PGA1_c10290 0.31 -0.5 1,066,788 - PGA1_c10290 0.33 -0.1 1,066,822 + PGA1_c10290 0.38 -2.3 1,066,843 + PGA1_c10290 0.42 -0.5 1,066,900 + PGA1_c10290 0.51 +1.0 1,067,006 + PGA1_c10290 0.69 +2.1 1,067,006 - PGA1_c10290 0.69 +0.4 1,067,011 + PGA1_c10290 0.70 +0.7 1,067,014 - PGA1_c10290 0.70 -0.5 1,067,014 - PGA1_c10290 0.70 -1.1 1,067,019 - PGA1_c10290 0.71 +1.0 1,067,114 + PGA1_c10290 0.87 +0.0 1,067,114 + PGA1_c10290 0.87 +0.1 1,067,122 - PGA1_c10290 0.88 +0.7 1,067,130 + PGA1_c10290 0.89 +2.9 1,067,130 + PGA1_c10290 0.89 -0.5 1,067,135 + -1.1 1,067,138 - -0.8 1,067,181 + -0.2 1,067,205 - -0.7 1,067,239 + -1.2 1,067,247 - +0.1 1,067,270 - -0.5 1,067,272 + -0.7 1,067,350 + +0.4 1,067,404 - +1.1 1,067,448 + -0.9 1,067,770 - PGA1_c10300 0.27 -0.6 1,067,831 + PGA1_c10300 0.30 -2.2 1,067,832 + PGA1_c10300 0.31 -0.1 1,067,876 + PGA1_c10300 0.33 -0.9 1,067,884 - PGA1_c10300 0.34 -1.8 1,067,996 + PGA1_c10300 0.41 -1.1 1,068,073 - PGA1_c10300 0.46 +1.3 1,068,129 + PGA1_c10300 0.50 -0.1 1,068,231 - PGA1_c10300 0.56 -2.1 1,068,267 - PGA1_c10300 0.59 -1.7 1,068,268 + PGA1_c10300 0.59 -0.9 1,068,276 - PGA1_c10300 0.59 -0.5 1,068,327 - PGA1_c10300 0.62 +1.3 1,068,394 + PGA1_c10300 0.67 +0.4 1,068,475 + PGA1_c10300 0.72 -0.5 1,068,597 - PGA1_c10300 0.80 -0.5 1,068,605 - PGA1_c10300 0.80 -0.7 1,068,722 + PGA1_c10300 0.88 +0.1 1,068,730 - PGA1_c10300 0.88 -0.1 1,068,730 - PGA1_c10300 0.88 +0.2 1,068,810 - -0.2 1,068,839 + +0.1 1,068,845 - +0.8 1,068,847 - +0.3 1,068,954 + +1.5 1,068,957 - +0.9 1,068,964 + +1.1 1,069,014 - -1.8 1,069,035 - -0.8 1,069,057 + -1.1 1,069,057 + +1.5 1,069,057 - -0.1 1,069,088 + PGA1_c10310 0.12 -1.0 1,069,114 + PGA1_c10310 0.20 +1.2 1,069,119 + PGA1_c10310 0.22 +0.5 1,069,119 + PGA1_c10310 0.22 +0.5 1,069,122 - PGA1_c10310 0.23 -0.5 1,069,122 - PGA1_c10310 0.23 -0.1 1,069,127 - PGA1_c10310 0.24 +0.6 1,069,127 - PGA1_c10310 0.24 +0.7 1,069,127 - PGA1_c10310 0.24 +0.5 1,069,129 + PGA1_c10310 0.25 -0.4 1,069,152 + PGA1_c10310 0.32 -1.0 1,069,152 + PGA1_c10310 0.32 -0.5 1,069,160 - PGA1_c10310 0.34 -2.3 1,069,160 - PGA1_c10310 0.34 +1.9 1,069,175 - PGA1_c10310 0.38 -0.2 1,069,253 + PGA1_c10310 0.62 +0.7 1,069,267 - PGA1_c10310 0.66 -0.8 1,069,267 - PGA1_c10310 0.66 +0.1 1,069,267 - PGA1_c10310 0.66 -2.3 1,069,267 - PGA1_c10310 0.66 -0.5 1,069,339 + PGA1_c10310 0.87 -1.3 1,069,355 + -0.5 1,069,361 + -2.0 1,069,443 - -1.1
Or see this region's nucleotide sequence