Experiment: 101 uM BPS, BHI 1x protoporphyrin for 24 hours.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4570 and BT4571 overlap by 4 nucleotides BT4571 and BT4572 are separated by 131 nucleotides BT4572 and BT4573 are separated by 179 nucleotides BT4573 and BT4574 are separated by 20 nucleotides
BT4570: BT4570 - hypothetical protein (NCBI ptt file), at 5,992,032 to 5,992,439
BT4570
BT4571: BT4571 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 5,992,436 to 5,992,996
BT4571
BT4572: BT4572 - enolase (NCBI ptt file), at 5,993,128 to 5,994,408
BT4572
BT4573: BT4573 - conserved hypothetical protein (NCBI ptt file), at 5,994,588 to 5,995,250
BT4573
BT4574: BT4574 - conserved hypothetical protein (NCBI ptt file), at 5,995,271 to 5,995,645
BT4574
Position (kb)
5993
5994
5995 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 5992.181 kb on + strand, within BT4570 at 5992.424 kb on + strand at 5992.468 kb on + strand at 5992.469 kb on - strand at 5992.472 kb on + strand at 5992.473 kb on - strand at 5992.531 kb on + strand, within BT4571 at 5992.612 kb on + strand, within BT4571 at 5992.613 kb on - strand, within BT4571 at 5992.613 kb on - strand, within BT4571 at 5992.664 kb on - strand, within BT4571 at 5992.773 kb on + strand, within BT4571 at 5993.014 kb on + strand at 5993.018 kb on - strand at 5993.019 kb on + strand at 5993.024 kb on + strand at 5993.029 kb on - strand at 5993.053 kb on - strand at 5993.130 kb on - strand at 5993.152 kb on + strand at 5993.199 kb on - strand at 5993.209 kb on - strand at 5993.322 kb on + strand, within BT4572 at 5993.325 kb on - strand, within BT4572 at 5993.467 kb on + strand, within BT4572 at 5993.643 kb on + strand, within BT4572 at 5993.644 kb on - strand, within BT4572 at 5993.644 kb on - strand, within BT4572 at 5993.645 kb on + strand, within BT4572 at 5993.645 kb on + strand, within BT4572 at 5993.652 kb on - strand, within BT4572 at 5993.652 kb on - strand, within BT4572 at 5993.656 kb on - strand, within BT4572 at 5993.857 kb on + strand, within BT4572 at 5993.967 kb on + strand, within BT4572 at 5993.993 kb on + strand, within BT4572 at 5993.994 kb on - strand, within BT4572 at 5993.997 kb on + strand, within BT4572 at 5993.998 kb on - strand, within BT4572 at 5994.015 kb on - strand, within BT4572 at 5994.038 kb on + strand, within BT4572 at 5994.070 kb on + strand, within BT4572 at 5994.070 kb on + strand, within BT4572 at 5994.095 kb on - strand, within BT4572 at 5994.114 kb on + strand, within BT4572 at 5994.169 kb on - strand, within BT4572 at 5994.334 kb on + strand at 5994.349 kb on + strand at 5994.427 kb on + strand at 5994.474 kb on - strand at 5994.475 kb on + strand at 5994.479 kb on + strand at 5994.480 kb on - strand at 5994.484 kb on - strand at 5994.505 kb on + strand at 5994.506 kb on - strand at 5994.516 kb on - strand at 5994.582 kb on - strand at 5994.602 kb on + strand at 5994.671 kb on + strand, within BT4573 at 5994.681 kb on + strand, within BT4573 at 5994.702 kb on - strand, within BT4573 at 5994.832 kb on - strand, within BT4573 at 5995.011 kb on + strand, within BT4573 at 5995.013 kb on + strand, within BT4573 at 5995.090 kb on + strand, within BT4573 at 5995.091 kb on - strand, within BT4573 at 5995.181 kb on + strand, within BT4573 at 5995.257 kb on + strand at 5995.257 kb on + strand at 5995.262 kb on - strand at 5995.290 kb on + strand at 5995.300 kb on - strand at 5995.306 kb on + strand at 5995.306 kb on + strand at 5995.307 kb on - strand at 5995.307 kb on - strand at 5995.334 kb on + strand, within BT4574 at 5995.335 kb on - strand, within BT4574 at 5995.338 kb on + strand, within BT4574
Per-strain Table
Position Strand Gene LocusTag Fraction 101 uM BPS, BHI 1x protoporphyrin for 24 hours. remove 5,992,181 + BT4570 0.37 -1.7 5,992,424 + -1.0 5,992,468 + +1.3 5,992,469 - +0.3 5,992,472 + -0.8 5,992,473 - -0.7 5,992,531 + BT4571 0.17 -0.7 5,992,612 + BT4571 0.31 -1.6 5,992,613 - BT4571 0.32 +0.3 5,992,613 - BT4571 0.32 -0.7 5,992,664 - BT4571 0.41 +2.3 5,992,773 + BT4571 0.60 -1.4 5,993,014 + -1.4 5,993,018 - -2.1 5,993,019 + -1.2 5,993,024 + -1.2 5,993,029 - -1.9 5,993,053 - -1.4 5,993,130 - -2.1 5,993,152 + -2.4 5,993,199 - -5.6 5,993,209 - -2.2 5,993,322 + BT4572 0.15 -5.0 5,993,325 - BT4572 0.15 -5.2 5,993,467 + BT4572 0.26 -2.7 5,993,643 + BT4572 0.40 +0.3 5,993,644 - BT4572 0.40 +0.3 5,993,644 - BT4572 0.40 -0.7 5,993,645 + BT4572 0.40 -4.0 5,993,645 + BT4572 0.40 -1.0 5,993,652 - BT4572 0.41 -1.7 5,993,652 - BT4572 0.41 +0.3 5,993,656 - BT4572 0.41 -2.9 5,993,857 + BT4572 0.57 +0.3 5,993,967 + BT4572 0.65 -3.3 5,993,993 + BT4572 0.68 -4.0 5,993,994 - BT4572 0.68 -0.3 5,993,997 + BT4572 0.68 -4.8 5,993,998 - BT4572 0.68 -1.4 5,994,015 - BT4572 0.69 -4.8 5,994,038 + BT4572 0.71 -2.9 5,994,070 + BT4572 0.74 -1.3 5,994,070 + BT4572 0.74 -3.0 5,994,095 - BT4572 0.75 -0.7 5,994,114 + BT4572 0.77 -2.6 5,994,169 - BT4572 0.81 -0.7 5,994,334 + -3.6 5,994,349 + -1.7 5,994,427 + -1.7 5,994,474 - -0.4 5,994,475 + -4.0 5,994,479 + +0.3 5,994,480 - -0.3 5,994,484 - -1.5 5,994,505 + -2.0 5,994,506 - -0.7 5,994,516 - +0.1 5,994,582 - -0.7 5,994,602 + -1.7 5,994,671 + BT4573 0.13 -2.4 5,994,681 + BT4573 0.14 -0.7 5,994,702 - BT4573 0.17 -0.6 5,994,832 - BT4573 0.37 +1.3 5,995,011 + BT4573 0.64 -0.7 5,995,013 + BT4573 0.64 +1.3 5,995,090 + BT4573 0.76 -2.1 5,995,091 - BT4573 0.76 +1.0 5,995,181 + BT4573 0.89 -0.6 5,995,257 + -1.1 5,995,257 + -0.1 5,995,262 - +0.3 5,995,290 + -0.7 5,995,300 - -1.0 5,995,306 + +1.9 5,995,306 + -1.3 5,995,307 - -1.6 5,995,307 - -0.7 5,995,334 + BT4574 0.17 -1.7 5,995,335 - BT4574 0.17 -2.2 5,995,338 + BT4574 0.18 +1.3
Or see this region's nucleotide sequence