Experiment: 0.15 mM H2O2 in BHI+1x protoporphyrin, cultured for 20 hours.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1218 and BT1219 are separated by 285 nucleotides BT1219 and BT1220 are separated by 184 nucleotides BT1220 and BT1221 are separated by 23 nucleotides
BT1218: BT1218 - conserved hypothetical protein, with conserved domain (NCBI ptt file), at 1,515,380 to 1,516,078
BT1218
BT1219: BT1219 - conserved hypothetical protein (NCBI ptt file), at 1,516,364 to 1,517,314
BT1219
BT1220: BT1220 - 6-phosphogluconolactonase (NCBI ptt file), at 1,517,499 to 1,518,188
BT1220
BT1221: BT1221 - glucose-6-phosphate 1-dehydrogenase (NCBI ptt file), at 1,518,212 to 1,519,708
BT1221
Position (kb)
1516
1517
1518 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1515.366 kb on + strand at 1515.367 kb on - strand at 1515.379 kb on + strand at 1515.485 kb on + strand, within BT1218 at 1515.520 kb on + strand, within BT1218 at 1515.544 kb on - strand, within BT1218 at 1515.607 kb on - strand, within BT1218 at 1515.741 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.749 kb on + strand, within BT1218 at 1515.750 kb on - strand, within BT1218 at 1515.751 kb on + strand, within BT1218 at 1515.751 kb on + strand, within BT1218 at 1515.752 kb on - strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.753 kb on + strand, within BT1218 at 1515.794 kb on + strand, within BT1218 at 1516.013 kb on + strand at 1516.013 kb on + strand at 1516.013 kb on + strand at 1516.013 kb on + strand at 1516.014 kb on - strand at 1516.014 kb on - strand at 1516.014 kb on - strand at 1516.077 kb on - strand at 1516.230 kb on - strand at 1516.340 kb on - strand at 1516.392 kb on + strand at 1516.430 kb on - strand at 1516.517 kb on - strand, within BT1219 at 1516.522 kb on - strand, within BT1219 at 1516.533 kb on + strand, within BT1219 at 1516.534 kb on - strand, within BT1219 at 1516.537 kb on + strand, within BT1219 at 1516.543 kb on - strand, within BT1219 at 1516.543 kb on - strand, within BT1219 at 1516.573 kb on + strand, within BT1219 at 1516.583 kb on + strand at 1516.584 kb on - strand, within BT1219 at 1516.584 kb on - strand, within BT1219 at 1516.584 kb on - strand, within BT1219 at 1516.584 kb on - strand, within BT1219 at 1516.672 kb on + strand, within BT1219 at 1516.729 kb on + strand, within BT1219 at 1516.741 kb on - strand, within BT1219 at 1516.794 kb on - strand, within BT1219 at 1517.018 kb on - strand, within BT1219 at 1517.019 kb on + strand, within BT1219 at 1517.020 kb on - strand, within BT1219 at 1517.131 kb on + strand, within BT1219 at 1517.132 kb on - strand, within BT1219 at 1517.156 kb on - strand, within BT1219 at 1517.183 kb on + strand, within BT1219 at 1517.183 kb on + strand, within BT1219 at 1517.183 kb on + strand, within BT1219 at 1517.184 kb on - strand, within BT1219 at 1517.184 kb on - strand, within BT1219 at 1517.192 kb on + strand, within BT1219 at 1517.230 kb on - strand at 1517.237 kb on - strand at 1517.241 kb on - strand at 1517.314 kb on + strand at 1517.322 kb on + strand at 1517.348 kb on + strand at 1517.365 kb on + strand at 1517.390 kb on + strand at 1517.391 kb on - strand at 1517.395 kb on - strand at 1517.395 kb on - strand at 1517.395 kb on - strand at 1517.447 kb on - strand at 1517.461 kb on + strand at 1517.462 kb on - strand at 1517.463 kb on + strand at 1517.465 kb on + strand at 1517.465 kb on + strand at 1517.466 kb on - strand at 1517.466 kb on - strand at 1517.469 kb on - strand at 1517.469 kb on - strand at 1517.470 kb on + strand at 1517.481 kb on + strand at 1517.515 kb on + strand at 1517.516 kb on - strand at 1517.568 kb on + strand at 1517.569 kb on - strand, within BT1220 at 1517.571 kb on - strand, within BT1220 at 1517.571 kb on - strand, within BT1220 at 1517.572 kb on + strand at 1517.573 kb on - strand, within BT1220 at 1517.775 kb on - strand, within BT1220 at 1517.783 kb on + strand at 1517.784 kb on - strand, within BT1220 at 1517.784 kb on - strand, within BT1220 at 1517.929 kb on + strand, within BT1220 at 1517.974 kb on + strand, within BT1220 at 1517.979 kb on - strand, within BT1220 at 1518.050 kb on + strand at 1518.115 kb on - strand, within BT1220 at 1518.226 kb on - strand at 1518.289 kb on + strand at 1518.290 kb on - strand at 1518.290 kb on - strand at 1518.290 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 0.15 mM H2O2 in BHI+1x protoporphyrin, cultured for 20 hours. remove 1,515,366 + -1.5 1,515,367 - +1.5 1,515,379 + -0.8 1,515,485 + BT1218 0.15 -0.9 1,515,520 + BT1218 0.20 +0.5 1,515,544 - BT1218 0.23 -2.0 1,515,607 - BT1218 0.32 -0.5 1,515,741 + BT1218 0.52 +0.1 1,515,749 + BT1218 0.53 -0.5 1,515,749 + BT1218 0.53 +1.5 1,515,749 + BT1218 0.53 -1.3 1,515,750 - BT1218 0.53 +0.3 1,515,751 + BT1218 0.53 +0.2 1,515,751 + BT1218 0.53 -0.5 1,515,752 - BT1218 0.53 -0.9 1,515,753 + BT1218 0.53 -0.6 1,515,753 + BT1218 0.53 -1.1 1,515,753 + BT1218 0.53 -1.1 1,515,794 + BT1218 0.59 -1.8 1,516,013 + -0.5 1,516,013 + -1.3 1,516,013 + +0.5 1,516,013 + -1.2 1,516,014 - -1.3 1,516,014 - -1.1 1,516,014 - +1.3 1,516,077 - +0.3 1,516,230 - -0.4 1,516,340 - -1.2 1,516,392 + -0.4 1,516,430 - -1.5 1,516,517 - BT1219 0.16 -0.5 1,516,522 - BT1219 0.17 -0.1 1,516,533 + BT1219 0.18 -1.5 1,516,534 - BT1219 0.18 -1.4 1,516,537 + BT1219 0.18 -0.1 1,516,543 - BT1219 0.19 -1.3 1,516,543 - BT1219 0.19 -0.8 1,516,573 + BT1219 0.22 -2.1 1,516,583 + -1.2 1,516,584 - BT1219 0.23 -1.9 1,516,584 - BT1219 0.23 +1.5 1,516,584 - BT1219 0.23 -2.5 1,516,584 - BT1219 0.23 -1.3 1,516,672 + BT1219 0.32 -0.1 1,516,729 + BT1219 0.38 -1.7 1,516,741 - BT1219 0.40 -0.5 1,516,794 - BT1219 0.45 -2.6 1,517,018 - BT1219 0.69 +0.3 1,517,019 + BT1219 0.69 +0.5 1,517,020 - BT1219 0.69 +0.5 1,517,131 + BT1219 0.81 -2.7 1,517,132 - BT1219 0.81 -2.0 1,517,156 - BT1219 0.83 -3.4 1,517,183 + BT1219 0.86 -0.1 1,517,183 + BT1219 0.86 +0.4 1,517,183 + BT1219 0.86 +0.2 1,517,184 - BT1219 0.86 +0.5 1,517,184 - BT1219 0.86 -0.6 1,517,192 + BT1219 0.87 -1.8 1,517,230 - +1.2 1,517,237 - +0.3 1,517,241 - -0.3 1,517,314 + +1.5 1,517,322 + +0.5 1,517,348 + -1.1 1,517,365 + -2.3 1,517,390 + +0.2 1,517,391 - -0.1 1,517,395 - -0.1 1,517,395 - +0.5 1,517,395 - -2.1 1,517,447 - -0.8 1,517,461 + -0.0 1,517,462 - -0.0 1,517,463 + -0.8 1,517,465 + -0.6 1,517,465 + +0.0 1,517,466 - -0.6 1,517,466 - +0.2 1,517,469 - -0.8 1,517,469 - +1.2 1,517,470 + -0.1 1,517,481 + -0.2 1,517,515 + -1.3 1,517,516 - -1.3 1,517,568 + +1.5 1,517,569 - BT1220 0.10 +1.5 1,517,571 - BT1220 0.10 -0.5 1,517,571 - BT1220 0.10 +0.5 1,517,572 + +1.5 1,517,573 - BT1220 0.11 +1.5 1,517,775 - BT1220 0.40 -2.1 1,517,783 + +0.9 1,517,784 - BT1220 0.41 +1.5 1,517,784 - BT1220 0.41 -1.5 1,517,929 + BT1220 0.62 +1.5 1,517,974 + BT1220 0.69 +0.5 1,517,979 - BT1220 0.70 +1.5 1,518,050 + -0.1 1,518,115 - BT1220 0.89 -0.5 1,518,226 - -0.1 1,518,289 + +1.5 1,518,290 - -1.3 1,518,290 - +0.5 1,518,290 - -0.5
Or see this region's nucleotide sequence