Experiment: 0.35mg/ml WT Calprotectin + 1uM saturated Fe-Enterobactin in 50% 50%BHI + 50% Calprotectin buffer + 1x protoporphyrin, cultured for 20 hours.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4204 and BT4205 are separated by 21 nucleotides BT4205 and BT4206 are separated by 10 nucleotides BT4206 and BT4207 are separated by 11 nucleotides
BT4204: BT4204 - conserved hypothetical protein (NCBI ptt file), at 5,540,900 to 5,541,454
BT4204
BT4205: BT4205 - acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (NCBI ptt file), at 5,541,476 to 5,542,243
BT4205
BT4206: BT4206 - UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase (NCBI ptt file), at 5,542,254 to 5,543,639
BT4206
BT4207: BT4207 - UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (NCBI ptt file), at 5,543,651 to 5,544,691
BT4207
Position (kb) 5542
5543
5544 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5541.478 kb on - strand at 5542.660 kb on - strand, within BT4206 at 5542.660 kb on - strand, within BT4206 at 5542.709 kb on - strand, within BT4206 at 5542.709 kb on - strand, within BT4206
Per-strain Table
Position Strand Gene LocusTag Fraction 0.35mg/ml WT Calprotectin + 1uM saturated Fe-Enterobactin in 50% 50%BHI + 50% Calprotectin buffer + 1x protoporphyrin, cultured for 20 hours. remove 5,541,478 - +2.9 5,542,660 - BT4206 0.29 -1.0 5,542,660 - BT4206 0.29 -0.3 5,542,709 - BT4206 0.33 -0.3 5,542,709 - BT4206 0.33 -1.8
Or see this region's nucleotide sequence