Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24995 and BWI76_RS25000 are separated by 11 nucleotides BWI76_RS25000 and BWI76_RS25005 overlap by 8 nucleotides BWI76_RS25005 and BWI76_RS25010 overlap by 1 nucleotides
BWI76_RS24995: BWI76_RS24995 - malonate decarboxylase subunit alpha, at 5,205,172 to 5,206,839
_RS24995
BWI76_RS25000: BWI76_RS25000 - malonate decarboxylase acyl carrier protein, at 5,206,851 to 5,207,183
_RS25000
BWI76_RS25005: BWI76_RS25005 - biotin-independent malonate decarboxylase subunit beta, at 5,207,176 to 5,208,105
_RS25005
BWI76_RS25010: BWI76_RS25010 - malonyl-S-acyl carrier protein--biotin carboxyltransferase, at 5,208,105 to 5,208,986
_RS25010
Position (kb) 5206
5207
5208 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5206.068 kb on + strand, within BWI76_RS24995 at 5206.137 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.152 kb on + strand, within BWI76_RS24995 at 5206.155 kb on - strand, within BWI76_RS24995 at 5206.192 kb on + strand, within BWI76_RS24995 at 5206.376 kb on - strand, within BWI76_RS24995 at 5206.641 kb on - strand, within BWI76_RS24995 at 5206.684 kb on + strand at 5206.806 kb on + strand at 5206.812 kb on - strand at 5206.854 kb on + strand at 5207.092 kb on - strand, within BWI76_RS25000 at 5207.131 kb on + strand, within BWI76_RS25000 at 5207.151 kb on - strand at 5207.296 kb on + strand, within BWI76_RS25005 at 5207.301 kb on + strand, within BWI76_RS25005 at 5207.339 kb on + strand, within BWI76_RS25005 at 5207.347 kb on - strand, within BWI76_RS25005 at 5207.549 kb on - strand, within BWI76_RS25005 at 5207.650 kb on - strand, within BWI76_RS25005 at 5208.002 kb on + strand, within BWI76_RS25005
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,206,068 + BWI76_RS24995 0.54 -1.4 5,206,137 + BWI76_RS24995 0.58 -0.2 5,206,152 + BWI76_RS24995 0.59 -1.7 5,206,152 + BWI76_RS24995 0.59 +0.8 5,206,155 - BWI76_RS24995 0.59 -1.2 5,206,192 + BWI76_RS24995 0.61 +0.8 5,206,376 - BWI76_RS24995 0.72 +0.5 5,206,641 - BWI76_RS24995 0.88 +0.2 5,206,684 + +3.0 5,206,806 + +0.7 5,206,812 - +0.9 5,206,854 + -0.2 5,207,092 - BWI76_RS25000 0.72 -1.3 5,207,131 + BWI76_RS25000 0.84 -1.8 5,207,151 - +0.7 5,207,296 + BWI76_RS25005 0.13 +0.2 5,207,301 + BWI76_RS25005 0.13 +0.4 5,207,339 + BWI76_RS25005 0.18 +0.3 5,207,347 - BWI76_RS25005 0.18 -1.8 5,207,549 - BWI76_RS25005 0.40 +0.1 5,207,650 - BWI76_RS25005 0.51 -0.8 5,208,002 + BWI76_RS25005 0.89 -1.3
Or see this region's nucleotide sequence