Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24695 and BWI76_RS24700 are separated by 77 nucleotides BWI76_RS24700 and BWI76_RS24705 are separated by 69 nucleotides BWI76_RS24705 and BWI76_RS24710 are separated by 250 nucleotides
BWI76_RS24695: BWI76_RS24695 - glycoside hydrolase 43 family protein, at 5,149,330 to 5,150,973
_RS24695
BWI76_RS24700: BWI76_RS24700 - hypothetical protein, at 5,151,051 to 5,151,554
_RS24700
BWI76_RS24705: BWI76_RS24705 - oxidoreductase, at 5,151,624 to 5,152,613
_RS24705
BWI76_RS24710: BWI76_RS24710 - membrane protein, at 5,152,864 to 5,153,832
_RS24710
Position (kb) 5151
5152
5153 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 5150.643 kb on + strand, within BWI76_RS24695 at 5150.733 kb on - strand, within BWI76_RS24695 at 5150.815 kb on + strand at 5150.823 kb on - strand at 5150.823 kb on - strand at 5150.823 kb on - strand at 5151.058 kb on + strand at 5151.321 kb on - strand, within BWI76_RS24700 at 5151.549 kb on - strand at 5151.576 kb on + strand at 5151.653 kb on + strand at 5151.738 kb on + strand, within BWI76_RS24705 at 5151.773 kb on + strand, within BWI76_RS24705 at 5151.805 kb on + strand, within BWI76_RS24705 at 5151.913 kb on + strand, within BWI76_RS24705 at 5151.926 kb on - strand, within BWI76_RS24705 at 5151.981 kb on - strand, within BWI76_RS24705 at 5152.049 kb on + strand, within BWI76_RS24705 at 5152.215 kb on + strand, within BWI76_RS24705 at 5152.223 kb on - strand, within BWI76_RS24705 at 5152.269 kb on + strand, within BWI76_RS24705 at 5152.284 kb on + strand, within BWI76_RS24705 at 5152.296 kb on + strand, within BWI76_RS24705 at 5152.304 kb on - strand, within BWI76_RS24705 at 5152.453 kb on + strand, within BWI76_RS24705 at 5152.453 kb on + strand, within BWI76_RS24705 at 5152.461 kb on - strand, within BWI76_RS24705 at 5152.463 kb on + strand, within BWI76_RS24705 at 5152.616 kb on - strand at 5152.616 kb on - strand at 5152.616 kb on - strand at 5152.917 kb on - strand at 5152.942 kb on - strand at 5152.975 kb on - strand, within BWI76_RS24710 at 5153.196 kb on - strand, within BWI76_RS24710 at 5153.460 kb on + strand, within BWI76_RS24710 at 5153.461 kb on + strand, within BWI76_RS24710 at 5153.521 kb on + strand, within BWI76_RS24710 at 5153.587 kb on - strand, within BWI76_RS24710
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,150,643 + BWI76_RS24695 0.80 +7.6 5,150,733 - BWI76_RS24695 0.85 +0.9 5,150,815 + -3.4 5,150,823 - +0.6 5,150,823 - +0.6 5,150,823 - -1.3 5,151,058 + -0.4 5,151,321 - BWI76_RS24700 0.54 +0.3 5,151,549 - +0.3 5,151,576 + -0.1 5,151,653 + -0.1 5,151,738 + BWI76_RS24705 0.12 +0.1 5,151,773 + BWI76_RS24705 0.15 +0.6 5,151,805 + BWI76_RS24705 0.18 -0.6 5,151,913 + BWI76_RS24705 0.29 -0.2 5,151,926 - BWI76_RS24705 0.31 -0.2 5,151,981 - BWI76_RS24705 0.36 +2.9 5,152,049 + BWI76_RS24705 0.43 +0.6 5,152,215 + BWI76_RS24705 0.60 -0.7 5,152,223 - BWI76_RS24705 0.61 -0.7 5,152,269 + BWI76_RS24705 0.65 +0.4 5,152,284 + BWI76_RS24705 0.67 +2.3 5,152,296 + BWI76_RS24705 0.68 +0.2 5,152,304 - BWI76_RS24705 0.69 -0.8 5,152,453 + BWI76_RS24705 0.84 +1.1 5,152,453 + BWI76_RS24705 0.84 -1.4 5,152,461 - BWI76_RS24705 0.85 +0.5 5,152,463 + BWI76_RS24705 0.85 -0.3 5,152,616 - -0.2 5,152,616 - +0.6 5,152,616 - -0.3 5,152,917 - -0.4 5,152,942 - +0.6 5,152,975 - BWI76_RS24710 0.11 -0.0 5,153,196 - BWI76_RS24710 0.34 +0.1 5,153,460 + BWI76_RS24710 0.62 -0.1 5,153,461 + BWI76_RS24710 0.62 -0.2 5,153,521 + BWI76_RS24710 0.68 +1.3 5,153,587 - BWI76_RS24710 0.75 -1.0
Or see this region's nucleotide sequence