Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22825 and BWI76_RS22830 are separated by 19 nucleotides BWI76_RS22830 and BWI76_RS22835 overlap by 4 nucleotides
BWI76_RS22825: BWI76_RS22825 - glucarate dehydratase, at 4,762,559 to 4,763,899
_RS22825
BWI76_RS22830: BWI76_RS22830 - glucarate dehydratase, at 4,763,919 to 4,765,259
_RS22830
BWI76_RS22835: BWI76_RS22835 - MFS transporter, at 4,765,256 to 4,766,614
_RS22835
Position (kb) 4763
4764
4765
4766 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.947 kb on + strand, within BWI76_RS22825 at 4762.955 kb on - strand, within BWI76_RS22825 at 4763.004 kb on - strand, within BWI76_RS22825 at 4763.005 kb on - strand at 4763.016 kb on - strand, within BWI76_RS22825 at 4763.096 kb on + strand, within BWI76_RS22825 at 4763.109 kb on - strand, within BWI76_RS22825 at 4763.109 kb on - strand, within BWI76_RS22825 at 4763.179 kb on + strand, within BWI76_RS22825 at 4763.981 kb on - strand at 4763.987 kb on - strand at 4764.014 kb on + strand at 4764.022 kb on - strand at 4764.022 kb on - strand at 4764.027 kb on - strand at 4764.032 kb on - strand at 4764.062 kb on - strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.071 kb on + strand, within BWI76_RS22830 at 4764.079 kb on - strand, within BWI76_RS22830 at 4764.253 kb on + strand, within BWI76_RS22830 at 4764.258 kb on + strand, within BWI76_RS22830 at 4764.511 kb on + strand, within BWI76_RS22830 at 4764.519 kb on - strand, within BWI76_RS22830 at 4764.647 kb on + strand, within BWI76_RS22830 at 4764.774 kb on + strand, within BWI76_RS22830 at 4764.802 kb on + strand, within BWI76_RS22830 at 4764.824 kb on - strand, within BWI76_RS22830 at 4764.886 kb on + strand, within BWI76_RS22830 at 4765.109 kb on + strand, within BWI76_RS22830 at 4765.227 kb on - strand at 4765.329 kb on + strand at 4765.330 kb on - strand at 4765.339 kb on + strand at 4765.361 kb on - strand at 4765.451 kb on - strand, within BWI76_RS22835 at 4765.785 kb on + strand, within BWI76_RS22835 at 4765.866 kb on + strand, within BWI76_RS22835 at 4765.938 kb on - strand, within BWI76_RS22835 at 4766.094 kb on - strand, within BWI76_RS22835 at 4766.145 kb on + strand, within BWI76_RS22835 at 4766.169 kb on + strand, within BWI76_RS22835
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,762,947 + BWI76_RS22825 0.29 +0.2 4,762,947 + BWI76_RS22825 0.29 +0.8 4,762,947 + BWI76_RS22825 0.29 -1.2 4,762,947 + BWI76_RS22825 0.29 +0.4 4,762,955 - BWI76_RS22825 0.30 -2.4 4,763,004 - BWI76_RS22825 0.33 -0.5 4,763,005 - -1.1 4,763,016 - BWI76_RS22825 0.34 -0.3 4,763,096 + BWI76_RS22825 0.40 +0.3 4,763,109 - BWI76_RS22825 0.41 -0.1 4,763,109 - BWI76_RS22825 0.41 -0.4 4,763,179 + BWI76_RS22825 0.46 +1.3 4,763,981 - -0.7 4,763,987 - -0.0 4,764,014 + +0.2 4,764,022 - -1.7 4,764,022 - -0.0 4,764,027 - +3.1 4,764,032 - -0.5 4,764,062 - BWI76_RS22830 0.11 -0.1 4,764,071 + BWI76_RS22830 0.11 -0.9 4,764,071 + BWI76_RS22830 0.11 +0.2 4,764,071 + BWI76_RS22830 0.11 -2.7 4,764,079 - BWI76_RS22830 0.12 -1.1 4,764,253 + BWI76_RS22830 0.25 +0.3 4,764,258 + BWI76_RS22830 0.25 +0.6 4,764,511 + BWI76_RS22830 0.44 +1.2 4,764,519 - BWI76_RS22830 0.45 -0.5 4,764,647 + BWI76_RS22830 0.54 +0.4 4,764,774 + BWI76_RS22830 0.64 +0.5 4,764,802 + BWI76_RS22830 0.66 +0.3 4,764,824 - BWI76_RS22830 0.67 -0.7 4,764,886 + BWI76_RS22830 0.72 +4.1 4,765,109 + BWI76_RS22830 0.89 +4.1 4,765,227 - +2.9 4,765,329 + +0.3 4,765,330 - +1.6 4,765,339 + -0.6 4,765,361 - -0.8 4,765,451 - BWI76_RS22835 0.14 -1.9 4,765,785 + BWI76_RS22835 0.39 -2.0 4,765,866 + BWI76_RS22835 0.45 -0.1 4,765,938 - BWI76_RS22835 0.50 -1.3 4,766,094 - BWI76_RS22835 0.62 -0.6 4,766,145 + BWI76_RS22835 0.65 +0.8 4,766,169 + BWI76_RS22835 0.67 +0.7
Or see this region's nucleotide sequence