Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19765 and BWI76_RS19770 are separated by 426 nucleotides BWI76_RS19770 and BWI76_RS19775 are separated by 190 nucleotides
BWI76_RS19765: BWI76_RS19765 - membrane protein, at 4,132,161 to 4,132,868
_RS19765
BWI76_RS19770: BWI76_RS19770 - bifunctional murein DD-endopeptidase/murein LD-carboxypeptidase, at 4,133,295 to 4,133,864
_RS19770
BWI76_RS19775: BWI76_RS19775 - phage resistance protein, at 4,134,055 to 4,135,617
_RS19775
Position (kb)
4133
4134 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 4132.295 kb on + strand, within BWI76_RS19765 at 4132.314 kb on + strand, within BWI76_RS19765 at 4132.374 kb on + strand, within BWI76_RS19765 at 4132.382 kb on - strand, within BWI76_RS19765 at 4132.446 kb on + strand, within BWI76_RS19765 at 4132.537 kb on - strand, within BWI76_RS19765 at 4132.585 kb on + strand, within BWI76_RS19765 at 4132.729 kb on - strand, within BWI76_RS19765 at 4132.775 kb on - strand, within BWI76_RS19765 at 4132.792 kb on - strand, within BWI76_RS19765 at 4132.810 kb on + strand at 4132.900 kb on + strand at 4133.020 kb on - strand at 4133.023 kb on + strand at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.124 kb on - strand at 4133.281 kb on + strand at 4133.289 kb on - strand at 4133.289 kb on - strand at 4133.358 kb on + strand, within BWI76_RS19770 at 4133.366 kb on - strand, within BWI76_RS19770 at 4133.847 kb on + strand at 4134.051 kb on + strand at 4134.059 kb on - strand at 4134.202 kb on + strand at 4134.202 kb on + strand at 4134.202 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 4,132,295 + BWI76_RS19765 0.19 +0.6 4,132,314 + BWI76_RS19765 0.22 -0.5 4,132,374 + BWI76_RS19765 0.30 +0.5 4,132,382 - BWI76_RS19765 0.31 -0.5 4,132,446 + BWI76_RS19765 0.40 +0.2 4,132,537 - BWI76_RS19765 0.53 +0.1 4,132,585 + BWI76_RS19765 0.60 +0.8 4,132,729 - BWI76_RS19765 0.80 +2.1 4,132,775 - BWI76_RS19765 0.87 +0.5 4,132,792 - BWI76_RS19765 0.89 -0.4 4,132,810 + +0.4 4,132,900 + -0.1 4,133,020 - +1.2 4,133,023 + +0.8 4,133,124 - -0.3 4,133,124 - -0.8 4,133,124 - -1.6 4,133,281 + -0.2 4,133,289 - -0.4 4,133,289 - -0.7 4,133,358 + BWI76_RS19770 0.11 +0.1 4,133,366 - BWI76_RS19770 0.12 +4.9 4,133,847 + -0.0 4,134,051 + -1.2 4,134,059 - +0.2 4,134,202 + +0.4 4,134,202 + -1.3 4,134,202 + +1.1
Or see this region's nucleotide sequence