Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19130 and BWI76_RS19135 are separated by 136 nucleotides BWI76_RS19135 and BWI76_RS19140 are separated by 127 nucleotides
BWI76_RS19130: BWI76_RS19130 - 3-methyladenine DNA glycosylase 2, at 3,976,972 to 3,977,820
_RS19130
BWI76_RS19135: BWI76_RS19135 - molecular chaperone, at 3,977,957 to 3,979,309
_RS19135
BWI76_RS19140: BWI76_RS19140 - citrate (Si)-synthase, at 3,979,437 to 3,980,723
_RS19140
Position (kb)
3977
3978
3979
3980 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3976.970 kb on - strand at 3977.038 kb on - strand at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.207 kb on - strand, within BWI76_RS19130 at 3977.303 kb on - strand, within BWI76_RS19130 at 3977.330 kb on + strand, within BWI76_RS19130 at 3977.414 kb on - strand, within BWI76_RS19130 at 3977.460 kb on - strand, within BWI76_RS19130 at 3977.521 kb on - strand, within BWI76_RS19130 at 3977.624 kb on - strand, within BWI76_RS19130 at 3977.809 kb on - strand at 3977.854 kb on + strand at 3977.862 kb on - strand at 3978.346 kb on - strand, within BWI76_RS19135 at 3978.508 kb on + strand, within BWI76_RS19135 at 3978.717 kb on + strand, within BWI76_RS19135 at 3978.946 kb on - strand, within BWI76_RS19135 at 3978.993 kb on - strand, within BWI76_RS19135 at 3979.128 kb on - strand, within BWI76_RS19135 at 3979.230 kb on - strand at 3979.335 kb on + strand at 3979.363 kb on + strand at 3979.390 kb on + strand at 3979.391 kb on - strand at 3979.609 kb on + strand, within BWI76_RS19140 at 3979.718 kb on - strand, within BWI76_RS19140 at 3979.790 kb on + strand, within BWI76_RS19140 at 3979.890 kb on - strand, within BWI76_RS19140 at 3980.306 kb on + strand, within BWI76_RS19140
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,976,970 - +3.2 3,977,038 - -0.7 3,977,207 - BWI76_RS19130 0.28 -1.5 3,977,207 - BWI76_RS19130 0.28 -0.3 3,977,303 - BWI76_RS19130 0.39 -1.1 3,977,330 + BWI76_RS19130 0.42 +0.0 3,977,414 - BWI76_RS19130 0.52 +0.2 3,977,460 - BWI76_RS19130 0.57 -1.7 3,977,521 - BWI76_RS19130 0.65 -0.0 3,977,624 - BWI76_RS19130 0.77 -0.0 3,977,809 - -0.8 3,977,854 + -0.4 3,977,862 - +1.0 3,978,346 - BWI76_RS19135 0.29 -1.0 3,978,508 + BWI76_RS19135 0.41 -0.4 3,978,717 + BWI76_RS19135 0.56 +0.1 3,978,946 - BWI76_RS19135 0.73 +0.2 3,978,993 - BWI76_RS19135 0.77 +1.2 3,979,128 - BWI76_RS19135 0.87 +5.6 3,979,230 - +0.7 3,979,335 + +0.5 3,979,363 + -0.5 3,979,390 + -0.3 3,979,391 - +1.1 3,979,609 + BWI76_RS19140 0.13 -1.7 3,979,718 - BWI76_RS19140 0.22 -2.5 3,979,790 + BWI76_RS19140 0.27 +0.0 3,979,890 - BWI76_RS19140 0.35 -1.2 3,980,306 + BWI76_RS19140 0.68 -1.1
Or see this region's nucleotide sequence