Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS18885

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotidesBWI76_RS18880 and BWI76_RS18885 are separated by 321 nucleotidesBWI76_RS18885 and BWI76_RS18890 overlap by 4 nucleotides BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745 _RS18875 BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299 _RS18880 BWI76_RS18885: BWI76_RS18885 - nitrogen fixation regulatory protein, at 3,915,621 to 3,917,108 _RS18885 BWI76_RS18890: BWI76_RS18890 - nif-specific transcriptional activator NifA, at 3,917,105 to 3,918,679 _RS18890 Position (kb) 3915 3916 3917 3918Strain fitness (log2 ratio) -2 -1 0 1at 3914.792 kb on + strandat 3914.813 kb on - strandat 3915.397 kb on - strandat 3915.641 kb on + strandat 3916.112 kb on + strand, within BWI76_RS18885at 3916.779 kb on - strand, within BWI76_RS18885at 3916.853 kb on - strand, within BWI76_RS18885at 3917.080 kb on + strandat 3917.098 kb on - strandat 3917.189 kb on + strandat 3917.189 kb on + strandat 3917.231 kb on + strandat 3917.256 kb on + strandat 3917.486 kb on - strand, within BWI76_RS18890at 3917.499 kb on - strand, within BWI76_RS18890at 3917.514 kb on + strand, within BWI76_RS18890at 3917.688 kb on - strand, within BWI76_RS18890at 3917.688 kb on - strand, within BWI76_RS18890at 3917.693 kb on - strand, within BWI76_RS18890at 3917.797 kb on - strand, within BWI76_RS18890at 3917.799 kb on + strand, within BWI76_RS18890at 3917.810 kb on - strand, within BWI76_RS18890at 3917.874 kb on - strand, within BWI76_RS18890at 3918.008 kb on - strand, within BWI76_RS18890

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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3,914,792 + +0.4
3,914,813 - -1.7
3,915,397 - -1.1
3,915,641 + -0.3
3,916,112 + BWI76_RS18885 0.33 -0.4
3,916,779 - BWI76_RS18885 0.78 -1.5
3,916,853 - BWI76_RS18885 0.83 -2.0
3,917,080 + -0.6
3,917,098 - -0.1
3,917,189 + -0.7
3,917,189 + +1.6
3,917,231 + +0.3
3,917,256 + +0.0
3,917,486 - BWI76_RS18890 0.24 -0.7
3,917,499 - BWI76_RS18890 0.25 +1.1
3,917,514 + BWI76_RS18890 0.26 +0.0
3,917,688 - BWI76_RS18890 0.37 -0.6
3,917,688 - BWI76_RS18890 0.37 +0.6
3,917,693 - BWI76_RS18890 0.37 -0.5
3,917,797 - BWI76_RS18890 0.44 -0.3
3,917,799 + BWI76_RS18890 0.44 +0.4
3,917,810 - BWI76_RS18890 0.45 +0.4
3,917,874 - BWI76_RS18890 0.49 +0.6
3,918,008 - BWI76_RS18890 0.57 +0.6

Or see this region's nucleotide sequence