Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18860 and BWI76_RS18865 overlap by 1 nucleotides BWI76_RS18865 and BWI76_RS18870 overlap by 4 nucleotides BWI76_RS18870 and BWI76_RS18875 overlap by 4 nucleotides BWI76_RS18875 and BWI76_RS18880 are separated by 23 nucleotides
BWI76_RS18860: BWI76_RS18860 - homocitrate synthase, at 3,912,103 to 3,913,245
_RS18860
BWI76_RS18865: BWI76_RS18865 - nitrogenase-stabilizing/protective protein NifW, at 3,913,245 to 3,913,505
_RS18865
BWI76_RS18870: BWI76_RS18870 - protein NifZ, at 3,913,502 to 3,913,948
_RS18870
BWI76_RS18875: BWI76_RS18875 - nitrogen fixation protein NifM, at 3,913,945 to 3,914,745
_RS18875
BWI76_RS18880: BWI76_RS18880 - flavodoxin, at 3,914,769 to 3,915,299
_RS18880
Position (kb)
3913
3914 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3912.636 kb on + strand, within BWI76_RS18860 at 3912.750 kb on + strand, within BWI76_RS18860 at 3912.954 kb on - strand, within BWI76_RS18860 at 3913.047 kb on + strand, within BWI76_RS18860 at 3913.104 kb on + strand, within BWI76_RS18860 at 3913.119 kb on + strand, within BWI76_RS18860 at 3913.380 kb on - strand, within BWI76_RS18865 at 3913.774 kb on - strand, within BWI76_RS18870 at 3913.910 kb on + strand at 3914.056 kb on + strand, within BWI76_RS18875 at 3914.312 kb on - strand, within BWI76_RS18875 at 3914.368 kb on + strand, within BWI76_RS18875 at 3914.464 kb on - strand, within BWI76_RS18875 at 3914.498 kb on - strand, within BWI76_RS18875 at 3914.598 kb on - strand, within BWI76_RS18875 at 3914.792 kb on + strand at 3914.813 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,912,636 + BWI76_RS18860 0.47 +2.6 3,912,750 + BWI76_RS18860 0.57 +0.0 3,912,954 - BWI76_RS18860 0.74 +0.1 3,913,047 + BWI76_RS18860 0.83 -0.1 3,913,104 + BWI76_RS18860 0.88 -0.1 3,913,119 + BWI76_RS18860 0.89 +0.5 3,913,380 - BWI76_RS18865 0.52 -0.6 3,913,774 - BWI76_RS18870 0.61 -1.8 3,913,910 + +0.6 3,914,056 + BWI76_RS18875 0.14 -0.5 3,914,312 - BWI76_RS18875 0.46 +0.3 3,914,368 + BWI76_RS18875 0.53 +1.6 3,914,464 - BWI76_RS18875 0.65 -0.1 3,914,498 - BWI76_RS18875 0.69 +0.3 3,914,598 - BWI76_RS18875 0.82 -1.6 3,914,792 + +0.4 3,914,813 - -1.7
Or see this region's nucleotide sequence