Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18030 and BWI76_RS18035 are separated by 18 nucleotides BWI76_RS18035 and BWI76_RS18040 are separated by 2 nucleotides BWI76_RS18040 and BWI76_RS18045 are separated by 14 nucleotides
BWI76_RS18030: BWI76_RS18030 - spore coat protein U, at 3,746,728 to 3,747,246
_RS18030
BWI76_RS18035: BWI76_RS18035 - fimbrial chaperone, at 3,747,265 to 3,748,029
_RS18035
BWI76_RS18040: BWI76_RS18040 - fimbrial assembly protein, at 3,748,032 to 3,750,362
_RS18040
BWI76_RS18045: BWI76_RS18045 - spore coat U domain-containing protein, at 3,750,377 to 3,751,336
_RS18045
Position (kb)
3748
3749
3750
3751 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3747.048 kb on + strand, within BWI76_RS18030 at 3747.161 kb on - strand, within BWI76_RS18030 at 3747.163 kb on - strand, within BWI76_RS18030 at 3747.240 kb on - strand at 3747.383 kb on + strand, within BWI76_RS18035 at 3747.643 kb on + strand, within BWI76_RS18035 at 3747.677 kb on + strand, within BWI76_RS18035 at 3747.829 kb on + strand, within BWI76_RS18035 at 3747.834 kb on + strand, within BWI76_RS18035 at 3747.847 kb on - strand, within BWI76_RS18035 at 3748.082 kb on + strand at 3748.318 kb on - strand, within BWI76_RS18040 at 3748.633 kb on + strand, within BWI76_RS18040 at 3748.682 kb on - strand, within BWI76_RS18040 at 3748.689 kb on + strand, within BWI76_RS18040 at 3748.702 kb on - strand, within BWI76_RS18040 at 3748.858 kb on - strand, within BWI76_RS18040 at 3748.872 kb on - strand, within BWI76_RS18040 at 3748.874 kb on + strand, within BWI76_RS18040 at 3748.877 kb on - strand, within BWI76_RS18040 at 3748.978 kb on - strand, within BWI76_RS18040 at 3749.107 kb on + strand, within BWI76_RS18040 at 3749.593 kb on + strand, within BWI76_RS18040 at 3749.601 kb on - strand, within BWI76_RS18040 at 3749.694 kb on - strand, within BWI76_RS18040 at 3750.140 kb on - strand at 3750.269 kb on - strand at 3750.397 kb on - strand at 3750.406 kb on + strand at 3750.457 kb on + strand at 3750.494 kb on + strand, within BWI76_RS18045 at 3750.509 kb on + strand, within BWI76_RS18045 at 3750.655 kb on + strand, within BWI76_RS18045 at 3750.690 kb on - strand, within BWI76_RS18045 at 3750.719 kb on - strand, within BWI76_RS18045 at 3750.726 kb on + strand, within BWI76_RS18045 at 3750.734 kb on - strand, within BWI76_RS18045 at 3750.920 kb on + strand, within BWI76_RS18045 at 3750.928 kb on - strand, within BWI76_RS18045 at 3751.332 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,747,048 + BWI76_RS18030 0.62 +0.4 3,747,161 - BWI76_RS18030 0.83 -0.3 3,747,163 - BWI76_RS18030 0.84 -0.8 3,747,240 - +0.0 3,747,383 + BWI76_RS18035 0.15 +0.3 3,747,643 + BWI76_RS18035 0.49 +0.6 3,747,677 + BWI76_RS18035 0.54 -0.7 3,747,829 + BWI76_RS18035 0.74 +0.2 3,747,834 + BWI76_RS18035 0.74 -0.1 3,747,847 - BWI76_RS18035 0.76 +0.1 3,748,082 + -1.4 3,748,318 - BWI76_RS18040 0.12 -0.7 3,748,633 + BWI76_RS18040 0.26 +0.8 3,748,682 - BWI76_RS18040 0.28 -0.9 3,748,689 + BWI76_RS18040 0.28 -0.2 3,748,702 - BWI76_RS18040 0.29 +0.7 3,748,858 - BWI76_RS18040 0.35 -0.4 3,748,872 - BWI76_RS18040 0.36 +0.6 3,748,874 + BWI76_RS18040 0.36 +0.7 3,748,877 - BWI76_RS18040 0.36 -0.9 3,748,978 - BWI76_RS18040 0.41 -0.0 3,749,107 + BWI76_RS18040 0.46 +4.6 3,749,593 + BWI76_RS18040 0.67 -0.0 3,749,601 - BWI76_RS18040 0.67 +1.4 3,749,694 - BWI76_RS18040 0.71 +0.3 3,750,140 - -0.7 3,750,269 - -0.2 3,750,397 - +0.3 3,750,406 + +0.1 3,750,457 + +1.0 3,750,494 + BWI76_RS18045 0.12 -0.4 3,750,509 + BWI76_RS18045 0.14 +0.3 3,750,655 + BWI76_RS18045 0.29 -1.0 3,750,690 - BWI76_RS18045 0.33 -2.0 3,750,719 - BWI76_RS18045 0.36 +0.4 3,750,726 + BWI76_RS18045 0.36 -1.4 3,750,734 - BWI76_RS18045 0.37 +0.2 3,750,920 + BWI76_RS18045 0.57 +0.9 3,750,928 - BWI76_RS18045 0.57 +0.4 3,751,332 + -0.7
Or see this region's nucleotide sequence