Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15400 and BWI76_RS15405 are separated by 195 nucleotides BWI76_RS15405 and BWI76_RS15410 are separated by 139 nucleotides BWI76_RS15410 and BWI76_RS15415 are separated by 386 nucleotides
BWI76_RS15400: BWI76_RS15400 - anti-RssB factor, at 3,226,476 to 3,226,787
_RS15400
BWI76_RS15405: BWI76_RS15405 - diguanylate phosphodiesterase, at 3,226,983 to 3,228,200
_RS15405
BWI76_RS15410: BWI76_RS15410 - N-acetyltransferase, at 3,228,340 to 3,228,774
_RS15410
BWI76_RS15415: BWI76_RS15415 - LacI family transcriptional regulator, at 3,229,161 to 3,230,231
_RS15415
Position (kb)
3226
3227
3228
3229 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3225.995 kb on - strand at 3226.008 kb on - strand at 3226.012 kb on + strand at 3226.014 kb on + strand at 3226.020 kb on - strand at 3226.020 kb on - strand at 3226.086 kb on - strand at 3226.088 kb on - strand at 3226.097 kb on + strand at 3226.196 kb on + strand at 3226.221 kb on - strand at 3226.254 kb on - strand at 3226.261 kb on + strand at 3226.269 kb on - strand at 3226.270 kb on + strand at 3226.270 kb on + strand at 3226.270 kb on + strand at 3226.290 kb on - strand at 3226.411 kb on - strand at 3226.469 kb on - strand at 3226.503 kb on - strand at 3226.550 kb on + strand at 3226.550 kb on + strand, within BWI76_RS15400 at 3226.558 kb on - strand, within BWI76_RS15400 at 3226.561 kb on + strand, within BWI76_RS15400 at 3226.562 kb on + strand, within BWI76_RS15400 at 3226.562 kb on + strand, within BWI76_RS15400 at 3226.570 kb on - strand, within BWI76_RS15400 at 3226.579 kb on - strand, within BWI76_RS15400 at 3226.648 kb on + strand, within BWI76_RS15400 at 3226.744 kb on - strand, within BWI76_RS15400 at 3226.809 kb on - strand at 3226.835 kb on + strand at 3226.835 kb on + strand at 3226.855 kb on + strand at 3226.858 kb on - strand at 3226.875 kb on + strand at 3226.908 kb on + strand at 3226.957 kb on - strand at 3227.013 kb on - strand at 3227.088 kb on - strand at 3227.099 kb on + strand at 3227.102 kb on - strand at 3227.107 kb on - strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.180 kb on + strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.188 kb on - strand, within BWI76_RS15405 at 3227.301 kb on + strand, within BWI76_RS15405 at 3227.378 kb on + strand, within BWI76_RS15405 at 3227.383 kb on + strand, within BWI76_RS15405 at 3227.383 kb on + strand, within BWI76_RS15405 at 3227.388 kb on + strand, within BWI76_RS15405 at 3227.441 kb on - strand, within BWI76_RS15405 at 3227.531 kb on - strand, within BWI76_RS15405 at 3227.576 kb on - strand, within BWI76_RS15405 at 3227.610 kb on - strand, within BWI76_RS15405 at 3227.630 kb on - strand, within BWI76_RS15405 at 3227.630 kb on - strand, within BWI76_RS15405 at 3227.855 kb on - strand, within BWI76_RS15405 at 3227.899 kb on - strand, within BWI76_RS15405 at 3227.976 kb on - strand, within BWI76_RS15405 at 3227.978 kb on + strand, within BWI76_RS15405 at 3227.991 kb on - strand, within BWI76_RS15405 at 3228.039 kb on - strand, within BWI76_RS15405 at 3228.039 kb on - strand, within BWI76_RS15405 at 3228.158 kb on + strand at 3228.158 kb on + strand at 3228.186 kb on - strand at 3228.197 kb on + strand at 3228.197 kb on + strand at 3228.197 kb on + strand at 3228.205 kb on - strand at 3228.205 kb on - strand at 3228.234 kb on - strand at 3228.236 kb on + strand at 3228.280 kb on - strand at 3228.298 kb on - strand at 3228.314 kb on - strand at 3228.513 kb on + strand, within BWI76_RS15410 at 3228.528 kb on - strand, within BWI76_RS15410 at 3228.534 kb on - strand, within BWI76_RS15410 at 3228.665 kb on + strand, within BWI76_RS15410 at 3228.690 kb on + strand, within BWI76_RS15410 at 3229.188 kb on - strand at 3229.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 3,225,995 - +1.0 3,226,008 - +0.7 3,226,012 + +1.2 3,226,014 + -0.6 3,226,020 - +0.4 3,226,020 - -0.1 3,226,086 - -0.5 3,226,088 - +1.4 3,226,097 + +1.4 3,226,196 + +0.3 3,226,221 - -1.2 3,226,254 - -0.3 3,226,261 + -0.5 3,226,269 - +1.9 3,226,270 + -2.3 3,226,270 + -1.7 3,226,270 + +0.3 3,226,290 - -0.8 3,226,411 - -0.5 3,226,469 - +1.0 3,226,503 - +1.7 3,226,550 + +0.3 3,226,550 + BWI76_RS15400 0.24 +1.4 3,226,558 - BWI76_RS15400 0.26 -0.5 3,226,561 + BWI76_RS15400 0.27 -0.2 3,226,562 + BWI76_RS15400 0.28 -0.3 3,226,562 + BWI76_RS15400 0.28 -0.3 3,226,570 - BWI76_RS15400 0.30 -0.2 3,226,579 - BWI76_RS15400 0.33 +0.5 3,226,648 + BWI76_RS15400 0.55 -0.2 3,226,744 - BWI76_RS15400 0.86 -0.2 3,226,809 - -2.8 3,226,835 + -0.8 3,226,835 + -0.7 3,226,855 + -0.5 3,226,858 - -0.5 3,226,875 + +0.6 3,226,908 + -1.4 3,226,957 - +1.0 3,227,013 - -0.2 3,227,088 - -1.1 3,227,099 + -1.7 3,227,102 - +3.3 3,227,107 - BWI76_RS15405 0.10 -0.2 3,227,180 + BWI76_RS15405 0.16 -1.7 3,227,180 + BWI76_RS15405 0.16 +0.7 3,227,180 + BWI76_RS15405 0.16 +0.3 3,227,180 + BWI76_RS15405 0.16 +0.2 3,227,180 + BWI76_RS15405 0.16 -0.7 3,227,180 + BWI76_RS15405 0.16 -1.0 3,227,180 + BWI76_RS15405 0.16 +0.2 3,227,188 - BWI76_RS15405 0.17 +3.2 3,227,188 - BWI76_RS15405 0.17 -0.9 3,227,188 - BWI76_RS15405 0.17 -0.6 3,227,188 - BWI76_RS15405 0.17 -1.2 3,227,188 - BWI76_RS15405 0.17 +0.7 3,227,188 - BWI76_RS15405 0.17 -0.9 3,227,188 - BWI76_RS15405 0.17 -0.5 3,227,188 - BWI76_RS15405 0.17 -0.8 3,227,188 - BWI76_RS15405 0.17 +0.6 3,227,188 - BWI76_RS15405 0.17 +2.5 3,227,188 - BWI76_RS15405 0.17 +0.4 3,227,188 - BWI76_RS15405 0.17 +0.5 3,227,188 - BWI76_RS15405 0.17 +0.1 3,227,188 - BWI76_RS15405 0.17 -0.2 3,227,188 - BWI76_RS15405 0.17 -0.6 3,227,301 + BWI76_RS15405 0.26 +0.2 3,227,378 + BWI76_RS15405 0.32 +3.8 3,227,383 + BWI76_RS15405 0.33 -1.2 3,227,383 + BWI76_RS15405 0.33 +0.4 3,227,388 + BWI76_RS15405 0.33 +1.4 3,227,441 - BWI76_RS15405 0.38 -0.5 3,227,531 - BWI76_RS15405 0.45 +0.4 3,227,576 - BWI76_RS15405 0.49 -1.5 3,227,610 - BWI76_RS15405 0.51 -0.3 3,227,630 - BWI76_RS15405 0.53 +0.0 3,227,630 - BWI76_RS15405 0.53 -0.0 3,227,855 - BWI76_RS15405 0.72 +0.7 3,227,899 - BWI76_RS15405 0.75 +4.3 3,227,976 - BWI76_RS15405 0.82 +2.1 3,227,978 + BWI76_RS15405 0.82 -0.2 3,227,991 - BWI76_RS15405 0.83 -0.6 3,228,039 - BWI76_RS15405 0.87 -0.2 3,228,039 - BWI76_RS15405 0.87 -0.4 3,228,158 + -0.3 3,228,158 + +0.1 3,228,186 - +0.1 3,228,197 + +0.8 3,228,197 + -1.2 3,228,197 + -1.3 3,228,205 - +0.5 3,228,205 - +0.2 3,228,234 - +0.7 3,228,236 + -0.2 3,228,280 - +0.0 3,228,298 - +1.2 3,228,314 - +2.7 3,228,513 + BWI76_RS15410 0.40 +0.5 3,228,528 - BWI76_RS15410 0.43 -0.6 3,228,534 - BWI76_RS15410 0.45 -0.3 3,228,665 + BWI76_RS15410 0.75 +4.2 3,228,690 + BWI76_RS15410 0.80 +0.6 3,229,188 - -0.2 3,229,188 - +0.7
Or see this region's nucleotide sequence