Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10645 and BWI76_RS10650 are separated by 125 nucleotides BWI76_RS10650 and BWI76_RS10655 are separated by 15 nucleotides BWI76_RS10655 and BWI76_RS10660 overlap by 35 nucleotides
BWI76_RS10645: BWI76_RS10645 - phytanoyl-CoA dioxygenase, at 2,247,972 to 2,248,739
_RS10645
BWI76_RS10650: BWI76_RS10650 - PTS fructose-like transporter subunit EIIB, at 2,248,865 to 2,249,206
_RS10650
BWI76_RS10655: BWI76_RS10655 - [formate-C-acetyltransferase]-activating enzyme, at 2,249,222 to 2,250,097
_RS10655
BWI76_RS10660: BWI76_RS10660 - formate C-acetyltransferase/glycerol dehydratase family glycyl radical enzyme, at 2,250,063 to 2,252,360
_RS10660
Position (kb)
2248
2249
2250 Strain fitness (log2 ratio)
-1
0
1
2 at 2247.922 kb on + strand at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.058 kb on + strand, within BWI76_RS10645 at 2248.071 kb on - strand, within BWI76_RS10645 at 2248.354 kb on + strand, within BWI76_RS10645 at 2248.411 kb on + strand, within BWI76_RS10645 at 2248.419 kb on - strand, within BWI76_RS10645 at 2248.419 kb on - strand, within BWI76_RS10645 at 2248.488 kb on + strand, within BWI76_RS10645 at 2249.028 kb on + strand, within BWI76_RS10650 at 2249.048 kb on - strand, within BWI76_RS10650 at 2249.148 kb on + strand, within BWI76_RS10650 at 2249.150 kb on + strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.156 kb on - strand, within BWI76_RS10650 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.389 kb on + strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2249.397 kb on - strand, within BWI76_RS10655 at 2250.094 kb on - strand at 2250.097 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,247,922 + +2.3 2,248,058 + BWI76_RS10645 0.11 +0.9 2,248,058 + BWI76_RS10645 0.11 -0.7 2,248,058 + BWI76_RS10645 0.11 +0.2 2,248,071 - BWI76_RS10645 0.13 +1.4 2,248,354 + BWI76_RS10645 0.50 +0.6 2,248,411 + BWI76_RS10645 0.57 -0.8 2,248,419 - BWI76_RS10645 0.58 +0.4 2,248,419 - BWI76_RS10645 0.58 +1.4 2,248,488 + BWI76_RS10645 0.67 -1.0 2,249,028 + BWI76_RS10650 0.48 -0.1 2,249,048 - BWI76_RS10650 0.54 -0.3 2,249,148 + BWI76_RS10650 0.83 +0.3 2,249,150 + BWI76_RS10650 0.83 +0.1 2,249,156 - BWI76_RS10650 0.85 -0.9 2,249,156 - BWI76_RS10650 0.85 +1.7 2,249,389 + BWI76_RS10655 0.19 +0.8 2,249,389 + BWI76_RS10655 0.19 +0.3 2,249,389 + BWI76_RS10655 0.19 +1.7 2,249,397 - BWI76_RS10655 0.20 +0.3 2,249,397 - BWI76_RS10655 0.20 +0.1 2,250,094 - -0.0 2,250,097 + +0.7
Or see this region's nucleotide sequence