Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10400 and BWI76_RS10405 are separated by 125 nucleotides BWI76_RS10405 and BWI76_RS10410 are separated by 234 nucleotides BWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotides BWI76_RS10415 and BWI76_RS10420 are separated by 190 nucleotides
BWI76_RS10400: BWI76_RS10400 - hypothetical protein, at 2,201,675 to 2,201,860
_RS10400
BWI76_RS10405: BWI76_RS10405 - cold-shock protein, at 2,201,986 to 2,202,207
_RS10405
BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702
_RS10410
BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171
_RS10415
BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390
_RS10420
Position (kb)
2202
2203
2204 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 2201.877 kb on + strand at 2201.933 kb on + strand at 2201.936 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.941 kb on - strand at 2201.965 kb on - strand at 2201.972 kb on + strand at 2201.986 kb on - strand at 2202.004 kb on + strand at 2202.007 kb on + strand at 2202.012 kb on - strand, within BWI76_RS10405 at 2202.017 kb on + strand, within BWI76_RS10405 at 2202.025 kb on - strand, within BWI76_RS10405 at 2202.027 kb on + strand, within BWI76_RS10405 at 2202.071 kb on - strand, within BWI76_RS10405 at 2202.082 kb on - strand, within BWI76_RS10405 at 2202.084 kb on + strand, within BWI76_RS10405 at 2202.086 kb on + strand, within BWI76_RS10405 at 2202.150 kb on + strand, within BWI76_RS10405 at 2202.153 kb on + strand, within BWI76_RS10405 at 2202.160 kb on + strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.161 kb on - strand, within BWI76_RS10405 at 2202.170 kb on + strand, within BWI76_RS10405 at 2202.171 kb on - strand, within BWI76_RS10405 at 2202.175 kb on + strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.183 kb on - strand, within BWI76_RS10405 at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.185 kb on + strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.193 kb on - strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.233 kb on + strand at 2202.241 kb on - strand at 2202.241 kb on - strand at 2202.254 kb on + strand at 2202.268 kb on + strand at 2202.273 kb on + strand at 2202.284 kb on + strand at 2202.299 kb on - strand at 2202.309 kb on + strand at 2202.309 kb on + strand at 2202.389 kb on + strand at 2202.394 kb on + strand at 2202.394 kb on + strand at 2202.442 kb on - strand at 2202.498 kb on - strand, within BWI76_RS10410 at 2202.610 kb on - strand, within BWI76_RS10410 at 2202.806 kb on - strand at 2202.835 kb on + strand, within BWI76_RS10415 at 2202.845 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2203.222 kb on - strand at 2203.281 kb on + strand at 2203.281 kb on + strand at 2203.289 kb on - strand at 2203.289 kb on - strand at 2203.625 kb on + strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.672 kb on + strand, within BWI76_RS10420 at 2203.729 kb on + strand, within BWI76_RS10420 at 2203.880 kb on + strand, within BWI76_RS10420
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 2,201,877 + -0.2 2,201,933 + -0.5 2,201,936 - +1.9 2,201,941 - +0.1 2,201,941 - +2.8 2,201,941 - -0.1 2,201,941 - -1.7 2,201,965 - +0.4 2,201,972 + +0.6 2,201,986 - -0.1 2,202,004 + +0.6 2,202,007 + +0.1 2,202,012 - BWI76_RS10405 0.12 -1.2 2,202,017 + BWI76_RS10405 0.14 -1.1 2,202,025 - BWI76_RS10405 0.18 -2.0 2,202,027 + BWI76_RS10405 0.18 +1.2 2,202,071 - BWI76_RS10405 0.38 +0.1 2,202,082 - BWI76_RS10405 0.43 +0.0 2,202,084 + BWI76_RS10405 0.44 +0.3 2,202,086 + BWI76_RS10405 0.45 +1.0 2,202,150 + BWI76_RS10405 0.74 +1.4 2,202,153 + BWI76_RS10405 0.75 -0.1 2,202,160 + BWI76_RS10405 0.78 +0.2 2,202,161 - BWI76_RS10405 0.79 +0.6 2,202,161 - BWI76_RS10405 0.79 +2.1 2,202,170 + BWI76_RS10405 0.83 -1.0 2,202,171 - BWI76_RS10405 0.83 +0.8 2,202,175 + BWI76_RS10405 0.85 -1.1 2,202,183 - BWI76_RS10405 0.89 -0.5 2,202,183 - BWI76_RS10405 0.89 +0.4 2,202,185 + -0.3 2,202,185 + +0.6 2,202,185 + -0.5 2,202,185 + +0.2 2,202,185 + -1.5 2,202,185 + +0.8 2,202,185 + +0.2 2,202,193 - +0.5 2,202,193 - -0.1 2,202,193 - -0.8 2,202,193 - +0.0 2,202,193 - +0.6 2,202,193 - -0.2 2,202,193 - +1.8 2,202,193 - -0.6 2,202,193 - +0.5 2,202,193 - +0.9 2,202,193 - +0.2 2,202,193 - -1.4 2,202,193 - +0.1 2,202,233 + -0.3 2,202,233 + +0.2 2,202,233 + +1.5 2,202,233 + +0.1 2,202,233 + +0.3 2,202,241 - +0.3 2,202,241 - +2.2 2,202,254 + +1.4 2,202,268 + -0.4 2,202,273 + -1.2 2,202,284 + -1.0 2,202,299 - -0.8 2,202,309 + -0.2 2,202,309 + -0.0 2,202,389 + -0.2 2,202,394 + +0.4 2,202,394 + +0.6 2,202,442 - +1.8 2,202,498 - BWI76_RS10410 0.21 +0.9 2,202,610 - BWI76_RS10410 0.64 +0.3 2,202,806 - +0.2 2,202,835 + BWI76_RS10415 0.11 -0.2 2,202,845 + BWI76_RS10415 0.13 +5.2 2,202,979 + BWI76_RS10415 0.49 -1.2 2,202,979 + BWI76_RS10415 0.49 +0.3 2,203,222 - +1.9 2,203,281 + +0.4 2,203,281 + -0.5 2,203,289 - -1.0 2,203,289 - +0.1 2,203,625 + BWI76_RS10420 0.26 +0.2 2,203,633 - BWI76_RS10420 0.26 +0.6 2,203,633 - BWI76_RS10420 0.26 +0.2 2,203,672 + BWI76_RS10420 0.30 -0.8 2,203,729 + BWI76_RS10420 0.36 -0.2 2,203,880 + BWI76_RS10420 0.50 -0.3
Or see this region's nucleotide sequence