Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07570 and BWI76_RS07575 are separated by 234 nucleotides BWI76_RS07575 and BWI76_RS07580 are separated by 49 nucleotides BWI76_RS07580 and BWI76_RS07585 are separated by 14 nucleotides
BWI76_RS07570: BWI76_RS07570 - GntR family transcriptional regulator with, at 1,602,984 to 1,604,450
_RS07570
BWI76_RS07575: BWI76_RS07575 - ABC transporter substrate-binding protein, at 1,604,685 to 1,605,536
_RS07575
BWI76_RS07580: BWI76_RS07580 - polar amino acid ABC transporter inner membrane subunit, at 1,605,586 to 1,606,227
_RS07580
BWI76_RS07585: BWI76_RS07585 - polar amino acid ABC transporter inner membrane subunit, at 1,606,242 to 1,606,907
_RS07585
Position (kb)
1604
1605
1606 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1604.095 kb on - strand, within BWI76_RS07570 at 1604.144 kb on - strand, within BWI76_RS07570 at 1604.216 kb on + strand, within BWI76_RS07570 at 1604.224 kb on - strand, within BWI76_RS07570 at 1604.238 kb on + strand, within BWI76_RS07570 at 1604.290 kb on - strand, within BWI76_RS07570 at 1604.339 kb on - strand at 1604.439 kb on - strand at 1604.476 kb on + strand at 1604.476 kb on + strand at 1604.484 kb on - strand at 1604.517 kb on - strand at 1604.539 kb on - strand at 1604.598 kb on + strand at 1604.598 kb on + strand at 1604.708 kb on - strand at 1604.719 kb on + strand at 1604.727 kb on - strand at 1604.727 kb on - strand at 1604.789 kb on + strand, within BWI76_RS07575 at 1604.820 kb on + strand, within BWI76_RS07575 at 1604.820 kb on + strand, within BWI76_RS07575 at 1604.828 kb on - strand, within BWI76_RS07575 at 1604.828 kb on - strand, within BWI76_RS07575 at 1604.865 kb on + strand, within BWI76_RS07575 at 1604.914 kb on + strand, within BWI76_RS07575 at 1604.915 kb on - strand, within BWI76_RS07575 at 1604.920 kb on - strand, within BWI76_RS07575 at 1604.922 kb on - strand, within BWI76_RS07575 at 1604.940 kb on - strand, within BWI76_RS07575 at 1604.955 kb on + strand, within BWI76_RS07575 at 1604.964 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.970 kb on + strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.978 kb on - strand, within BWI76_RS07575 at 1604.999 kb on - strand, within BWI76_RS07575 at 1605.001 kb on + strand, within BWI76_RS07575 at 1605.002 kb on + strand, within BWI76_RS07575 at 1605.009 kb on - strand, within BWI76_RS07575 at 1605.066 kb on + strand, within BWI76_RS07575 at 1605.074 kb on - strand, within BWI76_RS07575 at 1605.088 kb on + strand, within BWI76_RS07575 at 1605.088 kb on + strand, within BWI76_RS07575 at 1605.091 kb on - strand, within BWI76_RS07575 at 1605.217 kb on + strand, within BWI76_RS07575 at 1605.225 kb on - strand, within BWI76_RS07575 at 1605.227 kb on - strand, within BWI76_RS07575 at 1605.229 kb on + strand, within BWI76_RS07575 at 1605.293 kb on + strand, within BWI76_RS07575 at 1605.299 kb on + strand, within BWI76_RS07575 at 1605.312 kb on - strand, within BWI76_RS07575 at 1605.332 kb on - strand, within BWI76_RS07575 at 1605.391 kb on - strand, within BWI76_RS07575 at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.510 kb on - strand at 1605.536 kb on - strand at 1605.693 kb on + strand, within BWI76_RS07580 at 1605.726 kb on + strand, within BWI76_RS07580 at 1605.754 kb on - strand, within BWI76_RS07580 at 1605.919 kb on - strand, within BWI76_RS07580 at 1606.014 kb on + strand, within BWI76_RS07580 at 1606.048 kb on - strand, within BWI76_RS07580 at 1606.053 kb on - strand, within BWI76_RS07580 at 1606.112 kb on + strand, within BWI76_RS07580 at 1606.120 kb on - strand, within BWI76_RS07580 at 1606.173 kb on + strand at 1606.183 kb on + strand at 1606.188 kb on + strand at 1606.203 kb on - strand at 1606.203 kb on - strand at 1606.231 kb on - strand at 1606.234 kb on - strand at 1606.262 kb on - strand at 1606.269 kb on + strand at 1606.303 kb on + strand at 1606.311 kb on - strand, within BWI76_RS07585 at 1606.361 kb on - strand, within BWI76_RS07585 at 1606.489 kb on + strand, within BWI76_RS07585 at 1606.489 kb on + strand, within BWI76_RS07585 at 1606.496 kb on + strand, within BWI76_RS07585 at 1606.504 kb on - strand, within BWI76_RS07585 at 1606.504 kb on - strand, within BWI76_RS07585 at 1606.505 kb on - strand, within BWI76_RS07585 at 1606.527 kb on - strand, within BWI76_RS07585 at 1606.532 kb on - strand, within BWI76_RS07585
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,604,095 - BWI76_RS07570 0.76 -1.7 1,604,144 - BWI76_RS07570 0.79 -0.8 1,604,216 + BWI76_RS07570 0.84 +0.9 1,604,224 - BWI76_RS07570 0.85 -0.4 1,604,238 + BWI76_RS07570 0.85 -0.9 1,604,290 - BWI76_RS07570 0.89 -0.5 1,604,339 - +0.8 1,604,439 - -0.6 1,604,476 + +2.5 1,604,476 + -1.6 1,604,484 - +1.1 1,604,517 - -0.9 1,604,539 - -0.6 1,604,598 + -0.8 1,604,598 + +0.7 1,604,708 - -1.0 1,604,719 + +0.1 1,604,727 - -1.7 1,604,727 - -0.7 1,604,789 + BWI76_RS07575 0.12 -1.1 1,604,820 + BWI76_RS07575 0.16 +0.6 1,604,820 + BWI76_RS07575 0.16 +0.6 1,604,828 - BWI76_RS07575 0.17 -1.0 1,604,828 - BWI76_RS07575 0.17 +0.7 1,604,865 + BWI76_RS07575 0.21 -0.2 1,604,914 + BWI76_RS07575 0.27 +1.2 1,604,915 - BWI76_RS07575 0.27 +0.7 1,604,920 - BWI76_RS07575 0.28 +0.3 1,604,922 - BWI76_RS07575 0.28 +4.5 1,604,940 - BWI76_RS07575 0.30 -0.3 1,604,955 + BWI76_RS07575 0.32 +0.1 1,604,964 + BWI76_RS07575 0.33 -0.5 1,604,970 + BWI76_RS07575 0.33 +1.0 1,604,970 + BWI76_RS07575 0.33 -2.2 1,604,970 + BWI76_RS07575 0.33 +0.2 1,604,970 + BWI76_RS07575 0.33 -0.3 1,604,970 + BWI76_RS07575 0.33 +5.9 1,604,978 - BWI76_RS07575 0.34 -0.0 1,604,978 - BWI76_RS07575 0.34 -0.7 1,604,978 - BWI76_RS07575 0.34 -1.1 1,604,978 - BWI76_RS07575 0.34 -0.0 1,604,978 - BWI76_RS07575 0.34 -0.8 1,604,978 - BWI76_RS07575 0.34 +0.2 1,604,978 - BWI76_RS07575 0.34 +0.4 1,604,999 - BWI76_RS07575 0.37 -0.5 1,605,001 + BWI76_RS07575 0.37 -1.2 1,605,002 + BWI76_RS07575 0.37 -0.3 1,605,009 - BWI76_RS07575 0.38 -0.3 1,605,066 + BWI76_RS07575 0.45 -1.0 1,605,074 - BWI76_RS07575 0.46 +1.3 1,605,088 + BWI76_RS07575 0.47 -1.7 1,605,088 + BWI76_RS07575 0.47 -2.0 1,605,091 - BWI76_RS07575 0.48 +0.2 1,605,217 + BWI76_RS07575 0.62 +3.3 1,605,225 - BWI76_RS07575 0.63 +2.0 1,605,227 - BWI76_RS07575 0.64 +0.4 1,605,229 + BWI76_RS07575 0.64 -3.2 1,605,293 + BWI76_RS07575 0.71 -0.7 1,605,299 + BWI76_RS07575 0.72 -0.3 1,605,312 - BWI76_RS07575 0.74 +2.9 1,605,332 - BWI76_RS07575 0.76 -0.5 1,605,391 - BWI76_RS07575 0.83 +3.0 1,605,510 - -1.9 1,605,510 - -0.7 1,605,510 - -0.2 1,605,510 - -0.4 1,605,536 - +0.8 1,605,693 + BWI76_RS07580 0.17 +0.2 1,605,726 + BWI76_RS07580 0.22 -0.4 1,605,754 - BWI76_RS07580 0.26 +0.7 1,605,919 - BWI76_RS07580 0.52 +0.2 1,606,014 + BWI76_RS07580 0.67 -0.9 1,606,048 - BWI76_RS07580 0.72 -0.9 1,606,053 - BWI76_RS07580 0.73 -2.1 1,606,112 + BWI76_RS07580 0.82 +0.1 1,606,120 - BWI76_RS07580 0.83 -0.2 1,606,173 + -0.2 1,606,183 + +0.1 1,606,188 + +0.3 1,606,203 - -0.3 1,606,203 - -2.1 1,606,231 - +0.2 1,606,234 - -2.0 1,606,262 - +0.0 1,606,269 + -2.5 1,606,303 + -1.2 1,606,311 - BWI76_RS07585 0.10 +0.2 1,606,361 - BWI76_RS07585 0.18 -0.8 1,606,489 + BWI76_RS07585 0.37 +0.1 1,606,489 + BWI76_RS07585 0.37 -0.6 1,606,496 + BWI76_RS07585 0.38 -0.4 1,606,504 - BWI76_RS07585 0.39 -0.5 1,606,504 - BWI76_RS07585 0.39 -0.0 1,606,505 - BWI76_RS07585 0.39 -0.4 1,606,527 - BWI76_RS07585 0.43 -0.8 1,606,532 - BWI76_RS07585 0.44 -1.2
Or see this region's nucleotide sequence