Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05860 and BWI76_RS05865 are separated by 222 nucleotides BWI76_RS05865 and BWI76_RS05870 overlap by 4 nucleotides BWI76_RS05870 and BWI76_RS05875 are separated by 23 nucleotides
BWI76_RS05860: BWI76_RS05860 - alkanesulfonates-binding protein, at 1,230,772 to 1,231,839
_RS05860
BWI76_RS05865: BWI76_RS05865 - ABC transporter permease, at 1,232,062 to 1,233,630
_RS05865
BWI76_RS05870: BWI76_RS05870 - ABC transporter, at 1,233,627 to 1,234,370
_RS05870
BWI76_RS05875: BWI76_RS05875 - hypothetical protein, at 1,234,394 to 1,236,181
_RS05875
Position (kb)
1232
1233
1234 Strain fitness (log2 ratio)
-2
-1
0
1 at 1231.329 kb on + strand, within BWI76_RS05860 at 1231.517 kb on + strand, within BWI76_RS05860 at 1231.632 kb on + strand, within BWI76_RS05860 at 1232.345 kb on + strand, within BWI76_RS05865 at 1232.624 kb on + strand, within BWI76_RS05865 at 1232.639 kb on + strand, within BWI76_RS05865 at 1232.655 kb on + strand, within BWI76_RS05865 at 1232.785 kb on - strand, within BWI76_RS05865 at 1232.964 kb on + strand, within BWI76_RS05865 at 1233.062 kb on - strand, within BWI76_RS05865 at 1233.101 kb on - strand, within BWI76_RS05865 at 1233.143 kb on + strand, within BWI76_RS05865 at 1233.417 kb on + strand, within BWI76_RS05865 at 1233.562 kb on + strand at 1233.574 kb on + strand at 1233.575 kb on - strand at 1233.624 kb on + strand at 1233.624 kb on + strand at 1233.642 kb on - strand at 1233.680 kb on + strand at 1233.707 kb on + strand, within BWI76_RS05870 at 1233.760 kb on + strand, within BWI76_RS05870 at 1234.282 kb on + strand, within BWI76_RS05870 at 1234.295 kb on - strand, within BWI76_RS05870 at 1234.458 kb on + strand at 1234.493 kb on + strand at 1234.530 kb on + strand at 1234.586 kb on - strand, within BWI76_RS05875
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,231,329 + BWI76_RS05860 0.52 -1.1 1,231,517 + BWI76_RS05860 0.70 +0.1 1,231,632 + BWI76_RS05860 0.81 -0.6 1,232,345 + BWI76_RS05865 0.18 -1.0 1,232,624 + BWI76_RS05865 0.36 +0.1 1,232,639 + BWI76_RS05865 0.37 -0.3 1,232,655 + BWI76_RS05865 0.38 +1.1 1,232,785 - BWI76_RS05865 0.46 +0.8 1,232,964 + BWI76_RS05865 0.57 -0.9 1,233,062 - BWI76_RS05865 0.64 -1.1 1,233,101 - BWI76_RS05865 0.66 -1.9 1,233,143 + BWI76_RS05865 0.69 -0.4 1,233,417 + BWI76_RS05865 0.86 -0.7 1,233,562 + +0.2 1,233,574 + -0.7 1,233,575 - +0.3 1,233,624 + -0.6 1,233,624 + +0.5 1,233,642 - -1.4 1,233,680 + -0.4 1,233,707 + BWI76_RS05870 0.11 -1.4 1,233,760 + BWI76_RS05870 0.18 -0.6 1,234,282 + BWI76_RS05870 0.88 -1.8 1,234,295 - BWI76_RS05870 0.90 -2.2 1,234,458 + +1.3 1,234,493 + -0.5 1,234,530 + -0.2 1,234,586 - BWI76_RS05875 0.11 +0.3
Or see this region's nucleotide sequence