Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05565 and BWI76_RS05570 are separated by 2 nucleotides BWI76_RS05570 and BWI76_RS05575 overlap by 4 nucleotides BWI76_RS05575 and BWI76_RS05580 overlap by 4 nucleotides
BWI76_RS05565: BWI76_RS05565 - beta-galactosidase, at 1,165,171 to 1,166,589
_RS05565
BWI76_RS05570: BWI76_RS05570 - hypothetical protein, at 1,166,592 to 1,167,002
_RS05570
BWI76_RS05575: BWI76_RS05575 - group 1 glycosyl transferase, at 1,166,999 to 1,168,114
_RS05575
BWI76_RS05580: BWI76_RS05580 - group 1 glycosyl transferase, at 1,168,111 to 1,169,325
_RS05580
Position (kb) 1166
1167
1168
1169 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 1166.292 kb on - strand, within BWI76_RS05565 at 1166.406 kb on - strand, within BWI76_RS05565 at 1166.526 kb on + strand at 1166.557 kb on - strand at 1166.782 kb on + strand, within BWI76_RS05570 at 1166.981 kb on + strand at 1167.207 kb on - strand, within BWI76_RS05575 at 1167.224 kb on + strand, within BWI76_RS05575 at 1167.225 kb on + strand, within BWI76_RS05575 at 1167.250 kb on - strand, within BWI76_RS05575 at 1167.316 kb on + strand, within BWI76_RS05575 at 1167.591 kb on + strand, within BWI76_RS05575 at 1167.599 kb on - strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.814 kb on + strand, within BWI76_RS05575 at 1167.819 kb on + strand, within BWI76_RS05575 at 1167.822 kb on - strand, within BWI76_RS05575 at 1167.952 kb on - strand, within BWI76_RS05575 at 1168.096 kb on + strand at 1168.096 kb on - strand at 1168.109 kb on - strand at 1168.250 kb on + strand, within BWI76_RS05580 at 1168.425 kb on - strand, within BWI76_RS05580 at 1168.549 kb on - strand, within BWI76_RS05580 at 1168.808 kb on + strand, within BWI76_RS05580 at 1168.918 kb on + strand, within BWI76_RS05580 at 1168.978 kb on + strand, within BWI76_RS05580 at 1168.998 kb on + strand, within BWI76_RS05580 at 1169.078 kb on + strand, within BWI76_RS05580 at 1169.114 kb on + strand, within BWI76_RS05580
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 1,166,292 - BWI76_RS05565 0.79 +0.2 1,166,406 - BWI76_RS05565 0.87 +0.0 1,166,526 + +0.5 1,166,557 - -0.3 1,166,782 + BWI76_RS05570 0.46 +0.4 1,166,981 + -0.0 1,167,207 - BWI76_RS05575 0.19 -1.5 1,167,224 + BWI76_RS05575 0.20 -1.1 1,167,225 + BWI76_RS05575 0.20 +1.6 1,167,250 - BWI76_RS05575 0.22 +0.0 1,167,316 + BWI76_RS05575 0.28 +0.8 1,167,591 + BWI76_RS05575 0.53 +4.0 1,167,599 - BWI76_RS05575 0.54 +1.4 1,167,814 + BWI76_RS05575 0.73 -0.5 1,167,814 + BWI76_RS05575 0.73 -0.5 1,167,819 + BWI76_RS05575 0.73 +4.3 1,167,822 - BWI76_RS05575 0.74 +2.5 1,167,952 - BWI76_RS05575 0.85 +0.1 1,168,096 + -0.1 1,168,096 - -0.4 1,168,109 - -0.2 1,168,250 + BWI76_RS05580 0.11 -0.1 1,168,425 - BWI76_RS05580 0.26 +0.1 1,168,549 - BWI76_RS05580 0.36 -1.7 1,168,808 + BWI76_RS05580 0.57 +0.8 1,168,918 + BWI76_RS05580 0.66 +0.3 1,168,978 + BWI76_RS05580 0.71 -0.5 1,168,998 + BWI76_RS05580 0.73 +1.0 1,169,078 + BWI76_RS05580 0.80 +0.8 1,169,114 + BWI76_RS05580 0.83 -0.6
Or see this region's nucleotide sequence