Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04230 and BWI76_RS04235 are separated by 634 nucleotides BWI76_RS04235 and BWI76_RS04240 are separated by 212 nucleotides BWI76_RS04240 and BWI76_RS04245 are separated by 81 nucleotides
BWI76_RS04230: BWI76_RS04230 - DNA-binding response regulator, at 870,125 to 870,841
_RS04230
BWI76_RS04235: BWI76_RS04235 - tRNA/rRNA methyltransferase, at 871,476 to 872,162
_RS04235
BWI76_RS04240: BWI76_RS04240 - thr operon leader peptide, at 872,375 to 872,443
_RS04240
BWI76_RS04245: BWI76_RS04245 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 872,525 to 874,987
_RS04245
Position (kb) 871
872
873 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 870.897 kb on - strand at 870.897 kb on - strand at 871.043 kb on - strand at 871.080 kb on + strand at 871.088 kb on - strand at 871.088 kb on - strand at 871.145 kb on - strand at 871.147 kb on + strand at 871.155 kb on - strand at 871.155 kb on - strand at 871.156 kb on - strand at 871.255 kb on - strand at 871.321 kb on + strand at 871.337 kb on - strand at 871.620 kb on - strand, within BWI76_RS04235 at 871.624 kb on + strand, within BWI76_RS04235 at 871.632 kb on - strand, within BWI76_RS04235 at 871.784 kb on + strand, within BWI76_RS04235 at 871.868 kb on - strand, within BWI76_RS04235 at 871.870 kb on - strand, within BWI76_RS04235 at 871.886 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.903 kb on + strand, within BWI76_RS04235 at 871.911 kb on - strand, within BWI76_RS04235 at 871.930 kb on - strand, within BWI76_RS04235 at 872.053 kb on + strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.061 kb on - strand, within BWI76_RS04235 at 872.071 kb on + strand, within BWI76_RS04235 at 872.079 kb on - strand, within BWI76_RS04235 at 872.110 kb on + strand at 872.110 kb on + strand at 872.118 kb on + strand at 872.123 kb on - strand at 872.337 kb on + strand at 872.362 kb on + strand at 872.368 kb on - strand at 872.433 kb on - strand, within BWI76_RS04240 at 872.433 kb on - strand, within BWI76_RS04240 at 872.473 kb on + strand at 872.473 kb on + strand at 872.473 kb on + strand at 872.475 kb on + strand at 872.475 kb on + strand at 872.481 kb on - strand at 872.515 kb on + strand at 872.612 kb on - strand at 872.625 kb on + strand at 872.754 kb on - strand at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.844 kb on + strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.852 kb on - strand, within BWI76_RS04245 at 872.882 kb on - strand, within BWI76_RS04245 at 872.935 kb on + strand, within BWI76_RS04245
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 870,897 - -0.2 870,897 - -1.4 871,043 - -1.7 871,080 + -1.3 871,088 - -0.3 871,088 - +0.5 871,145 - -1.0 871,147 + -0.1 871,155 - +0.2 871,155 - -0.4 871,156 - -1.0 871,255 - +0.0 871,321 + -1.8 871,337 - -0.2 871,620 - BWI76_RS04235 0.21 +0.5 871,624 + BWI76_RS04235 0.22 -1.3 871,632 - BWI76_RS04235 0.23 +0.3 871,784 + BWI76_RS04235 0.45 +0.0 871,868 - BWI76_RS04235 0.57 -0.7 871,870 - BWI76_RS04235 0.57 -1.7 871,886 + BWI76_RS04235 0.60 -1.1 871,903 + BWI76_RS04235 0.62 -1.1 871,903 + BWI76_RS04235 0.62 -0.3 871,911 - BWI76_RS04235 0.63 -1.0 871,930 - BWI76_RS04235 0.66 -1.0 872,053 + BWI76_RS04235 0.84 -0.0 872,061 - BWI76_RS04235 0.85 +0.5 872,061 - BWI76_RS04235 0.85 -1.6 872,071 + BWI76_RS04235 0.87 -0.6 872,079 - BWI76_RS04235 0.88 -0.0 872,110 + -0.7 872,110 + -0.6 872,118 + +0.0 872,123 - -0.1 872,337 + -0.4 872,362 + +0.6 872,368 - +1.8 872,433 - BWI76_RS04240 0.84 +0.2 872,433 - BWI76_RS04240 0.84 +1.3 872,473 + +0.4 872,473 + -0.9 872,473 + -0.1 872,475 + +2.8 872,475 + +0.7 872,481 - +0.3 872,515 + -1.2 872,612 - +3.8 872,625 + -0.2 872,754 - -0.8 872,844 + BWI76_RS04245 0.13 -0.1 872,844 + BWI76_RS04245 0.13 +0.3 872,844 + BWI76_RS04245 0.13 -0.7 872,844 + BWI76_RS04245 0.13 +0.2 872,852 - BWI76_RS04245 0.13 -1.3 872,852 - BWI76_RS04245 0.13 +0.2 872,852 - BWI76_RS04245 0.13 +0.6 872,852 - BWI76_RS04245 0.13 +1.4 872,852 - BWI76_RS04245 0.13 +0.6 872,882 - BWI76_RS04245 0.14 +0.1 872,935 + BWI76_RS04245 0.17 -1.2
Or see this region's nucleotide sequence