Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03745 and BWI76_RS03750 are separated by 82 nucleotides BWI76_RS03750 and BWI76_RS03755 are separated by 373 nucleotides BWI76_RS03755 and BWI76_RS03760 overlap by 1 nucleotides BWI76_RS03760 and BWI76_RS03765 are separated by 10 nucleotides
BWI76_RS03745: BWI76_RS03745 - putative 2-hydroxyglutaryl-CoA dehydratase, at 775,567 to 776,718
_RS03745
BWI76_RS03750: BWI76_RS03750 - hypothetical protein, at 776,801 to 777,040
_RS03750
BWI76_RS03755: BWI76_RS03755 - phosphoadenosine phosphosulfate reductase, at 777,414 to 778,625
_RS03755
BWI76_RS03760: BWI76_RS03760 - hypothetical protein, at 778,625 to 779,251
_RS03760
BWI76_RS03765: BWI76_RS03765 - MFS transporter, at 779,262 to 780,503
_RS03765
Position (kb) 777
778
779 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 776.492 kb on - strand, within BWI76_RS03745 at 776.589 kb on + strand, within BWI76_RS03745 at 776.597 kb on - strand, within BWI76_RS03745 at 776.597 kb on - strand, within BWI76_RS03745 at 776.606 kb on + strand at 776.616 kb on + strand at 776.623 kb on + strand at 776.682 kb on + strand at 776.705 kb on - strand at 776.762 kb on + strand at 776.826 kb on - strand, within BWI76_RS03750 at 776.837 kb on - strand, within BWI76_RS03750 at 776.843 kb on + strand, within BWI76_RS03750 at 776.848 kb on + strand, within BWI76_RS03750 at 776.851 kb on - strand, within BWI76_RS03750 at 776.851 kb on - strand, within BWI76_RS03750 at 776.851 kb on - strand, within BWI76_RS03750 at 776.855 kb on + strand, within BWI76_RS03750 at 776.856 kb on - strand, within BWI76_RS03750 at 776.859 kb on - strand, within BWI76_RS03750 at 776.863 kb on - strand, within BWI76_RS03750 at 776.870 kb on - strand, within BWI76_RS03750 at 776.900 kb on + strand, within BWI76_RS03750 at 776.900 kb on + strand, within BWI76_RS03750 at 776.900 kb on + strand, within BWI76_RS03750 at 776.901 kb on - strand, within BWI76_RS03750 at 776.911 kb on - strand, within BWI76_RS03750 at 776.959 kb on + strand, within BWI76_RS03750 at 776.978 kb on + strand, within BWI76_RS03750 at 776.978 kb on + strand, within BWI76_RS03750 at 776.987 kb on - strand, within BWI76_RS03750 at 776.989 kb on + strand, within BWI76_RS03750 at 776.989 kb on + strand, within BWI76_RS03750 at 776.997 kb on - strand, within BWI76_RS03750 at 776.997 kb on - strand, within BWI76_RS03750 at 776.997 kb on - strand, within BWI76_RS03750 at 776.999 kb on + strand, within BWI76_RS03750 at 777.001 kb on + strand, within BWI76_RS03750 at 777.009 kb on - strand, within BWI76_RS03750 at 777.009 kb on - strand, within BWI76_RS03750 at 777.009 kb on - strand, within BWI76_RS03750 at 777.025 kb on + strand at 777.027 kb on - strand at 777.035 kb on + strand at 777.036 kb on + strand at 777.044 kb on - strand at 777.083 kb on + strand at 777.083 kb on + strand at 777.083 kb on + strand at 777.083 kb on + strand at 777.091 kb on - strand at 777.091 kb on - strand at 777.091 kb on - strand at 777.119 kb on - strand at 777.137 kb on + strand at 777.137 kb on + strand at 777.145 kb on - strand at 777.175 kb on - strand at 777.182 kb on + strand at 777.182 kb on + strand at 777.191 kb on - strand at 777.191 kb on - strand at 777.191 kb on - strand at 777.191 kb on - strand at 777.192 kb on + strand at 777.270 kb on + strand at 777.278 kb on - strand at 777.280 kb on - strand at 777.294 kb on + strand at 777.294 kb on + strand at 777.294 kb on + strand at 777.302 kb on - strand at 777.302 kb on - strand at 777.308 kb on + strand at 777.329 kb on + strand at 777.329 kb on + strand at 777.329 kb on + strand at 777.329 kb on + strand at 777.329 kb on + strand at 777.337 kb on - strand at 777.337 kb on - strand at 777.337 kb on - strand at 777.337 kb on - strand at 777.337 kb on - strand at 777.337 kb on - strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.339 kb on + strand at 777.347 kb on - strand at 777.347 kb on - strand at 777.347 kb on - strand at 777.347 kb on - strand at 777.347 kb on - strand at 777.347 kb on - strand at 777.376 kb on + strand at 777.384 kb on - strand at 777.404 kb on + strand at 777.406 kb on + strand at 777.406 kb on + strand at 777.407 kb on + strand at 777.414 kb on - strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.453 kb on + strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.461 kb on - strand at 777.462 kb on + strand at 777.462 kb on + strand at 777.467 kb on + strand at 777.467 kb on + strand at 777.470 kb on - strand at 777.473 kb on + strand at 777.476 kb on + strand at 777.494 kb on + strand at 777.507 kb on - strand at 777.531 kb on + strand at 777.531 kb on + strand at 777.539 kb on - strand, within BWI76_RS03755 at 777.545 kb on + strand, within BWI76_RS03755 at 777.545 kb on + strand, within BWI76_RS03755 at 777.552 kb on + strand, within BWI76_RS03755 at 777.585 kb on + strand, within BWI76_RS03755 at 777.593 kb on - strand, within BWI76_RS03755 at 777.599 kb on - strand, within BWI76_RS03755 at 777.619 kb on - strand, within BWI76_RS03755 at 777.624 kb on + strand, within BWI76_RS03755 at 777.653 kb on + strand, within BWI76_RS03755 at 777.655 kb on + strand, within BWI76_RS03755 at 777.655 kb on + strand, within BWI76_RS03755 at 777.663 kb on - strand, within BWI76_RS03755 at 777.663 kb on - strand, within BWI76_RS03755 at 777.663 kb on - strand, within BWI76_RS03755 at 777.705 kb on + strand, within BWI76_RS03755 at 777.707 kb on - strand, within BWI76_RS03755 at 777.712 kb on + strand, within BWI76_RS03755 at 777.712 kb on + strand, within BWI76_RS03755 at 777.712 kb on + strand, within BWI76_RS03755 at 777.712 kb on + strand, within BWI76_RS03755 at 777.754 kb on + strand, within BWI76_RS03755 at 777.769 kb on + strand, within BWI76_RS03755 at 777.944 kb on - strand, within BWI76_RS03755 at 778.013 kb on + strand, within BWI76_RS03755 at 778.034 kb on - strand, within BWI76_RS03755 at 778.079 kb on + strand, within BWI76_RS03755 at 778.089 kb on - strand, within BWI76_RS03755 at 778.174 kb on + strand, within BWI76_RS03755 at 778.225 kb on - strand, within BWI76_RS03755 at 778.279 kb on + strand, within BWI76_RS03755 at 778.287 kb on - strand, within BWI76_RS03755 at 778.559 kb on + strand at 778.566 kb on - strand at 778.568 kb on - strand at 778.576 kb on - strand at 778.627 kb on - strand at 778.724 kb on - strand, within BWI76_RS03760 at 778.806 kb on + strand, within BWI76_RS03760 at 778.806 kb on + strand, within BWI76_RS03760 at 778.814 kb on - strand, within BWI76_RS03760 at 778.874 kb on - strand, within BWI76_RS03760 at 778.933 kb on + strand, within BWI76_RS03760 at 778.973 kb on + strand, within BWI76_RS03760 at 779.072 kb on + strand, within BWI76_RS03760 at 779.121 kb on + strand, within BWI76_RS03760 at 779.242 kb on - strand at 779.622 kb on + strand, within BWI76_RS03765
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 776,492 - BWI76_RS03745 0.80 -0.3 776,589 + BWI76_RS03745 0.89 -0.2 776,597 - BWI76_RS03745 0.89 +1.1 776,597 - BWI76_RS03745 0.89 -0.6 776,606 + -0.3 776,616 + +0.9 776,623 + -1.6 776,682 + -2.5 776,705 - -0.1 776,762 + +0.0 776,826 - BWI76_RS03750 0.10 -0.1 776,837 - BWI76_RS03750 0.15 -0.5 776,843 + BWI76_RS03750 0.17 -0.7 776,848 + BWI76_RS03750 0.20 -1.8 776,851 - BWI76_RS03750 0.21 +0.8 776,851 - BWI76_RS03750 0.21 +0.2 776,851 - BWI76_RS03750 0.21 +0.1 776,855 + BWI76_RS03750 0.23 +0.3 776,856 - BWI76_RS03750 0.23 -2.3 776,859 - BWI76_RS03750 0.24 -0.5 776,863 - BWI76_RS03750 0.26 -1.1 776,870 - BWI76_RS03750 0.29 -0.4 776,900 + BWI76_RS03750 0.41 +0.9 776,900 + BWI76_RS03750 0.41 +0.3 776,900 + BWI76_RS03750 0.41 -2.5 776,901 - BWI76_RS03750 0.42 +0.1 776,911 - BWI76_RS03750 0.46 -1.5 776,959 + BWI76_RS03750 0.66 +4.8 776,978 + BWI76_RS03750 0.74 +0.3 776,978 + BWI76_RS03750 0.74 -0.8 776,987 - BWI76_RS03750 0.78 +0.4 776,989 + BWI76_RS03750 0.78 -1.3 776,989 + BWI76_RS03750 0.78 +1.0 776,997 - BWI76_RS03750 0.82 -0.2 776,997 - BWI76_RS03750 0.82 +1.4 776,997 - BWI76_RS03750 0.82 +1.0 776,999 + BWI76_RS03750 0.82 +0.3 777,001 + BWI76_RS03750 0.83 -0.1 777,009 - BWI76_RS03750 0.87 +1.3 777,009 - BWI76_RS03750 0.87 +0.5 777,009 - BWI76_RS03750 0.87 -0.2 777,025 + -0.3 777,027 - -1.1 777,035 + -0.8 777,036 + +0.6 777,044 - +0.9 777,083 + +0.5 777,083 + +1.0 777,083 + +0.3 777,083 + +0.3 777,091 - +0.3 777,091 - +0.3 777,091 - +0.9 777,119 - +0.4 777,137 + +0.9 777,137 + -0.9 777,145 - -0.1 777,175 - -1.2 777,182 + -0.2 777,182 + -2.8 777,191 - -1.1 777,191 - +0.2 777,191 - +1.0 777,191 - +1.3 777,192 + +2.1 777,270 + +0.7 777,278 - +0.8 777,280 - +0.8 777,294 + +0.1 777,294 + +0.7 777,294 + -0.5 777,302 - +0.9 777,302 - -0.8 777,308 + +0.3 777,329 + -0.7 777,329 + -0.3 777,329 + -0.2 777,329 + -0.9 777,329 + +0.3 777,337 - -1.7 777,337 - +0.2 777,337 - -0.5 777,337 - -0.5 777,337 - -0.5 777,337 - -0.1 777,339 + +1.9 777,339 + -1.8 777,339 + -1.5 777,339 + +0.5 777,339 + +0.7 777,339 + +0.3 777,339 + +1.0 777,339 + -1.0 777,339 + +0.3 777,347 - +0.3 777,347 - +0.3 777,347 - -1.3 777,347 - -0.5 777,347 - -0.0 777,347 - +1.0 777,376 + +0.6 777,384 - -0.1 777,404 + -0.1 777,406 + +1.1 777,406 + -0.1 777,407 + +0.0 777,414 - +2.4 777,453 + -0.6 777,453 + +0.3 777,453 + +1.1 777,453 + -0.6 777,453 + -0.3 777,453 + -0.3 777,453 + -0.2 777,453 + -0.3 777,453 + -0.2 777,453 + -0.5 777,453 + +0.8 777,453 + -0.1 777,453 + +0.9 777,453 + -0.3 777,453 + -0.8 777,453 + -0.0 777,453 + -0.6 777,453 + -1.3 777,453 + -1.7 777,453 + +1.5 777,453 + -0.2 777,453 + +0.7 777,453 + -0.3 777,453 + +0.7 777,453 + -1.0 777,453 + -1.5 777,461 - +0.3 777,461 - -1.9 777,461 - -1.1 777,461 - -1.0 777,461 - -2.0 777,461 - -0.1 777,461 - -0.5 777,461 - +0.1 777,461 - +0.8 777,461 - +0.8 777,461 - -0.7 777,461 - +1.9 777,461 - -0.6 777,461 - +1.3 777,461 - -0.1 777,461 - -0.7 777,461 - +0.8 777,461 - +0.2 777,461 - +0.5 777,461 - -0.9 777,461 - -0.6 777,461 - +1.6 777,462 + +0.5 777,462 + -0.4 777,467 + -0.1 777,467 + +0.2 777,470 - -0.2 777,473 + -1.2 777,476 + -0.1 777,494 + -0.6 777,507 - +0.9 777,531 + +0.4 777,531 + -1.6 777,539 - BWI76_RS03755 0.10 +0.6 777,545 + BWI76_RS03755 0.11 +1.4 777,545 + BWI76_RS03755 0.11 +2.2 777,552 + BWI76_RS03755 0.11 -0.7 777,585 + BWI76_RS03755 0.14 +0.1 777,593 - BWI76_RS03755 0.15 +0.1 777,599 - BWI76_RS03755 0.15 +0.2 777,619 - BWI76_RS03755 0.17 +1.6 777,624 + BWI76_RS03755 0.17 +0.5 777,653 + BWI76_RS03755 0.20 -0.3 777,655 + BWI76_RS03755 0.20 +2.0 777,655 + BWI76_RS03755 0.20 -0.0 777,663 - BWI76_RS03755 0.21 -0.6 777,663 - BWI76_RS03755 0.21 -0.2 777,663 - BWI76_RS03755 0.21 +0.5 777,705 + BWI76_RS03755 0.24 +0.1 777,707 - BWI76_RS03755 0.24 +3.5 777,712 + BWI76_RS03755 0.25 +0.5 777,712 + BWI76_RS03755 0.25 +0.4 777,712 + BWI76_RS03755 0.25 -1.4 777,712 + BWI76_RS03755 0.25 -0.5 777,754 + BWI76_RS03755 0.28 -0.9 777,769 + BWI76_RS03755 0.29 +0.8 777,944 - BWI76_RS03755 0.44 -0.6 778,013 + BWI76_RS03755 0.49 -0.7 778,034 - BWI76_RS03755 0.51 +0.2 778,079 + BWI76_RS03755 0.55 +1.3 778,089 - BWI76_RS03755 0.56 -0.1 778,174 + BWI76_RS03755 0.63 -0.8 778,225 - BWI76_RS03755 0.67 +0.1 778,279 + BWI76_RS03755 0.71 +0.5 778,287 - BWI76_RS03755 0.72 -0.6 778,559 + +0.0 778,566 - +1.9 778,568 - -0.0 778,576 - +1.3 778,627 - +0.9 778,724 - BWI76_RS03760 0.16 +1.1 778,806 + BWI76_RS03760 0.29 -0.7 778,806 + BWI76_RS03760 0.29 -0.5 778,814 - BWI76_RS03760 0.30 -0.1 778,874 - BWI76_RS03760 0.40 -0.9 778,933 + BWI76_RS03760 0.49 -0.2 778,973 + BWI76_RS03760 0.56 +0.1 779,072 + BWI76_RS03760 0.71 -0.5 779,121 + BWI76_RS03760 0.79 -0.9 779,242 - -1.0 779,622 + BWI76_RS03765 0.29 -0.1
Or see this region's nucleotide sequence