Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02105 and BWI76_RS02110 are separated by 36 nucleotides BWI76_RS02110 and BWI76_RS02115 are separated by 203 nucleotides BWI76_RS02115 and BWI76_RS02120 are separated by 198 nucleotides
BWI76_RS02105: BWI76_RS02105 - glutamate/aspartate:proton symporter GltP, at 434,204 to 435,517
_RS02105
BWI76_RS02110: BWI76_RS02110 - hypothetical protein, at 435,554 to 436,237
_RS02110
BWI76_RS02115: BWI76_RS02115 - formate dehydrogenase subunit alpha, at 436,441 to 438,588
_RS02115
BWI76_RS02120: BWI76_RS02120 - multidrug resistance transporter, at 438,787 to 440,250
_RS02120
Position (kb) 436
437
438
439 Strain fitness (log2 ratio) -1
0
1
2
3 at 435.480 kb on + strand at 435.553 kb on - strand at 435.572 kb on + strand at 435.612 kb on - strand at 435.786 kb on + strand, within BWI76_RS02110 at 435.814 kb on - strand, within BWI76_RS02110 at 435.864 kb on - strand, within BWI76_RS02110 at 435.879 kb on + strand, within BWI76_RS02110 at 436.084 kb on - strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.122 kb on + strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.130 kb on - strand, within BWI76_RS02110 at 436.267 kb on - strand at 436.269 kb on + strand at 436.269 kb on + strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.277 kb on - strand at 436.278 kb on - strand at 436.287 kb on - strand at 436.288 kb on - strand at 436.421 kb on + strand at 436.442 kb on - strand at 436.493 kb on + strand at 436.501 kb on + strand at 436.633 kb on - strand at 436.661 kb on + strand, within BWI76_RS02115 at 436.661 kb on + strand, within BWI76_RS02115 at 436.679 kb on - strand, within BWI76_RS02115 at 436.904 kb on - strand, within BWI76_RS02115 at 436.962 kb on + strand, within BWI76_RS02115 at 436.962 kb on + strand, within BWI76_RS02115 at 436.970 kb on - strand, within BWI76_RS02115 at 437.012 kb on + strand, within BWI76_RS02115 at 437.319 kb on + strand, within BWI76_RS02115 at 437.365 kb on + strand, within BWI76_RS02115 at 437.366 kb on + strand, within BWI76_RS02115 at 437.397 kb on - strand, within BWI76_RS02115 at 437.477 kb on + strand, within BWI76_RS02115 at 437.484 kb on - strand, within BWI76_RS02115 at 437.606 kb on - strand, within BWI76_RS02115 at 437.696 kb on - strand, within BWI76_RS02115 at 437.760 kb on + strand, within BWI76_RS02115 at 437.763 kb on - strand, within BWI76_RS02115 at 437.784 kb on + strand, within BWI76_RS02115 at 437.851 kb on - strand, within BWI76_RS02115 at 437.911 kb on - strand, within BWI76_RS02115 at 437.911 kb on - strand, within BWI76_RS02115 at 438.064 kb on - strand, within BWI76_RS02115 at 438.438 kb on + strand at 438.849 kb on + strand at 438.986 kb on + strand, within BWI76_RS02120 at 439.067 kb on + strand, within BWI76_RS02120 at 439.091 kb on - strand, within BWI76_RS02120 at 439.113 kb on - strand, within BWI76_RS02120 at 439.132 kb on - strand, within BWI76_RS02120 at 439.168 kb on + strand, within BWI76_RS02120 at 439.249 kb on - strand, within BWI76_RS02120 at 439.309 kb on + strand, within BWI76_RS02120 at 439.388 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 435,480 + -0.1 435,553 - +2.7 435,572 + +1.2 435,612 - -1.0 435,786 + BWI76_RS02110 0.34 -0.1 435,814 - BWI76_RS02110 0.38 -1.0 435,864 - BWI76_RS02110 0.45 -0.4 435,879 + BWI76_RS02110 0.48 +0.2 436,084 - BWI76_RS02110 0.77 -0.2 436,122 + BWI76_RS02110 0.83 +3.6 436,122 + BWI76_RS02110 0.83 -0.8 436,122 + BWI76_RS02110 0.83 -0.6 436,122 + BWI76_RS02110 0.83 -1.0 436,122 + BWI76_RS02110 0.83 +0.3 436,122 + BWI76_RS02110 0.83 +3.5 436,130 - BWI76_RS02110 0.84 +0.8 436,130 - BWI76_RS02110 0.84 -0.4 436,130 - BWI76_RS02110 0.84 +0.4 436,267 - -0.9 436,269 + -0.6 436,269 + +0.6 436,277 - +1.2 436,277 - -0.4 436,277 - +0.8 436,277 - -0.1 436,277 - -0.9 436,277 - -1.2 436,277 - -1.1 436,278 - +1.5 436,287 - +0.1 436,288 - +0.2 436,421 + -0.7 436,442 - +0.3 436,493 + +0.1 436,501 + -0.2 436,633 - -0.9 436,661 + BWI76_RS02115 0.10 +0.2 436,661 + BWI76_RS02115 0.10 -0.1 436,679 - BWI76_RS02115 0.11 +1.8 436,904 - BWI76_RS02115 0.22 +1.6 436,962 + BWI76_RS02115 0.24 -0.1 436,962 + BWI76_RS02115 0.24 +0.4 436,970 - BWI76_RS02115 0.25 +1.6 437,012 + BWI76_RS02115 0.27 +0.2 437,319 + BWI76_RS02115 0.41 +2.2 437,365 + BWI76_RS02115 0.43 +1.6 437,366 + BWI76_RS02115 0.43 +0.8 437,397 - BWI76_RS02115 0.45 -0.1 437,477 + BWI76_RS02115 0.48 -1.4 437,484 - BWI76_RS02115 0.49 -0.4 437,606 - BWI76_RS02115 0.54 -0.9 437,696 - BWI76_RS02115 0.58 -0.7 437,760 + BWI76_RS02115 0.61 +1.9 437,763 - BWI76_RS02115 0.62 +1.9 437,784 + BWI76_RS02115 0.63 +1.4 437,851 - BWI76_RS02115 0.66 +0.7 437,911 - BWI76_RS02115 0.68 -0.4 437,911 - BWI76_RS02115 0.68 +0.9 438,064 - BWI76_RS02115 0.76 -0.3 438,438 + -0.5 438,849 + +0.8 438,986 + BWI76_RS02120 0.14 +1.7 439,067 + BWI76_RS02120 0.19 -1.1 439,091 - BWI76_RS02120 0.21 -1.5 439,113 - BWI76_RS02120 0.22 -0.6 439,132 - BWI76_RS02120 0.24 -0.3 439,168 + BWI76_RS02120 0.26 +0.2 439,249 - BWI76_RS02120 0.32 -0.3 439,309 + BWI76_RS02120 0.36 -0.3 439,388 - BWI76_RS02120 0.41 -0.4 439,582 - BWI76_RS02120 0.54 -1.6 439,582 - BWI76_RS02120 0.54 -0.1 439,582 - BWI76_RS02120 0.54 +0.7
Or see this region's nucleotide sequence