Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00790 and BWI76_RS00795 are separated by 39 nucleotides BWI76_RS00795 and BWI76_RS00800 are separated by 91 nucleotides
BWI76_RS00790: BWI76_RS00790 - bifunctional aspartokinase II/homoserine dehydrogenase II, at 160,976 to 163,408
_RS00790
BWI76_RS00795: BWI76_RS00795 - carboxymuconolactone decarboxylase, at 163,448 to 163,819
_RS00795
BWI76_RS00800: BWI76_RS00800 - LysR family transcriptional regulator, at 163,911 to 164,816
_RS00800
Position (kb) 163
164 Strain fitness (log2 ratio) -2
-1
0
1
2 at 162.769 kb on + strand, within BWI76_RS00790 at 162.812 kb on - strand, within BWI76_RS00790 at 162.843 kb on + strand, within BWI76_RS00790 at 162.911 kb on - strand, within BWI76_RS00790 at 163.095 kb on + strand, within BWI76_RS00790 at 163.183 kb on + strand at 163.191 kb on - strand at 163.379 kb on + strand at 163.613 kb on - strand, within BWI76_RS00795 at 163.650 kb on - strand, within BWI76_RS00795 at 163.740 kb on - strand, within BWI76_RS00795 at 163.778 kb on + strand, within BWI76_RS00795 at 163.799 kb on + strand at 163.822 kb on - strand at 163.839 kb on - strand at 163.866 kb on + strand at 163.889 kb on - strand at 164.060 kb on + strand at 164.154 kb on + strand, within BWI76_RS00800 at 164.233 kb on + strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800 at 164.241 kb on - strand, within BWI76_RS00800 at 164.464 kb on + strand, within BWI76_RS00800 at 164.734 kb on - strand at 164.739 kb on - strand at 164.745 kb on - strand at 164.791 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 162,769 + BWI76_RS00790 0.74 -1.0 162,812 - BWI76_RS00790 0.75 -0.2 162,843 + BWI76_RS00790 0.77 +1.1 162,911 - BWI76_RS00790 0.80 +1.6 163,095 + BWI76_RS00790 0.87 +0.2 163,183 + -0.4 163,191 - -2.0 163,379 + -0.1 163,613 - BWI76_RS00795 0.44 -0.3 163,650 - BWI76_RS00795 0.54 +2.2 163,740 - BWI76_RS00795 0.78 -0.3 163,778 + BWI76_RS00795 0.89 +1.8 163,799 + -1.9 163,822 - +0.1 163,839 - +0.0 163,866 + -1.7 163,889 - +0.3 164,060 + +0.9 164,154 + BWI76_RS00800 0.27 +1.2 164,233 + BWI76_RS00800 0.36 +0.3 164,241 - BWI76_RS00800 0.36 -1.1 164,241 - BWI76_RS00800 0.36 -1.2 164,464 + BWI76_RS00800 0.61 +0.2 164,734 - -0.3 164,739 - +2.2 164,745 - -0.5 164,791 - +0.2
Or see this region's nucleotide sequence