Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27350 and BWI76_RS27355 are separated by 198 nucleotides BWI76_RS27355 and BWI76_RS27360 are separated by 20 nucleotides BWI76_RS27360 and BWI76_RS27365 are separated by 216 nucleotides BWI76_RS27365 and BWI76_RS27370 are separated by 175 nucleotides
BWI76_RS27350: BWI76_RS27350 - hypothetical protein, at 5,677,498 to 5,678,175
_RS27350
BWI76_RS27355: BWI76_RS27355 - 50S ribosomal protein L33, at 5,678,374 to 5,678,541
_RS27355
BWI76_RS27360: BWI76_RS27360 - 50S ribosomal protein L28, at 5,678,562 to 5,678,798
_RS27360
BWI76_RS27365: BWI76_RS27365 - hypothetical protein, at 5,679,015 to 5,679,680
_RS27365
BWI76_RS27370: BWI76_RS27370 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 5,679,856 to 5,681,067
_RS27370
Position (kb)
5679
5680 Strain fitness (log2 ratio)
-2
-1
0
1 at 5678.120 kb on + strand at 5678.121 kb on - strand at 5678.130 kb on + strand at 5678.321 kb on - strand at 5679.146 kb on + strand, within BWI76_RS27365 at 5679.146 kb on + strand, within BWI76_RS27365 at 5679.188 kb on - strand, within BWI76_RS27365 at 5679.397 kb on + strand, within BWI76_RS27365 at 5679.447 kb on - strand, within BWI76_RS27365 at 5679.480 kb on - strand, within BWI76_RS27365 at 5679.605 kb on + strand, within BWI76_RS27365 at 5679.711 kb on - strand at 5679.755 kb on + strand at 5679.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,678,120 + +0.6 5,678,121 - +0.2 5,678,130 + -1.2 5,678,321 - +0.0 5,679,146 + BWI76_RS27365 0.20 -0.2 5,679,146 + BWI76_RS27365 0.20 +1.7 5,679,188 - BWI76_RS27365 0.26 -2.3 5,679,397 + BWI76_RS27365 0.57 -0.5 5,679,447 - BWI76_RS27365 0.65 -0.3 5,679,480 - BWI76_RS27365 0.70 -1.1 5,679,605 + BWI76_RS27365 0.89 -0.6 5,679,711 - -1.4 5,679,755 + -0.3 5,679,816 - -0.3
Or see this region's nucleotide sequence