Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS27365

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS27350 and BWI76_RS27355 are separated by 198 nucleotidesBWI76_RS27355 and BWI76_RS27360 are separated by 20 nucleotidesBWI76_RS27360 and BWI76_RS27365 are separated by 216 nucleotidesBWI76_RS27365 and BWI76_RS27370 are separated by 175 nucleotides BWI76_RS27350: BWI76_RS27350 - hypothetical protein, at 5,677,498 to 5,678,175 _RS27350 BWI76_RS27355: BWI76_RS27355 - 50S ribosomal protein L33, at 5,678,374 to 5,678,541 _RS27355 BWI76_RS27360: BWI76_RS27360 - 50S ribosomal protein L28, at 5,678,562 to 5,678,798 _RS27360 BWI76_RS27365: BWI76_RS27365 - hypothetical protein, at 5,679,015 to 5,679,680 _RS27365 BWI76_RS27370: BWI76_RS27370 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 5,679,856 to 5,681,067 _RS27370 Position (kb) 5679 5680Strain fitness (log2 ratio) -2 -1 0 1at 5678.120 kb on + strandat 5678.121 kb on - strandat 5678.130 kb on + strandat 5678.321 kb on - strandat 5679.146 kb on + strand, within BWI76_RS27365at 5679.146 kb on + strand, within BWI76_RS27365at 5679.188 kb on - strand, within BWI76_RS27365at 5679.397 kb on + strand, within BWI76_RS27365at 5679.447 kb on - strand, within BWI76_RS27365at 5679.480 kb on - strand, within BWI76_RS27365at 5679.605 kb on + strand, within BWI76_RS27365at 5679.711 kb on - strandat 5679.755 kb on + strandat 5679.816 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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5,678,120 + +0.6
5,678,121 - +0.2
5,678,130 + -1.2
5,678,321 - +0.0
5,679,146 + BWI76_RS27365 0.20 -0.2
5,679,146 + BWI76_RS27365 0.20 +1.7
5,679,188 - BWI76_RS27365 0.26 -2.3
5,679,397 + BWI76_RS27365 0.57 -0.5
5,679,447 - BWI76_RS27365 0.65 -0.3
5,679,480 - BWI76_RS27365 0.70 -1.1
5,679,605 + BWI76_RS27365 0.89 -0.6
5,679,711 - -1.4
5,679,755 + -0.3
5,679,816 - -0.3

Or see this region's nucleotide sequence