Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26495 and BWI76_RS26500 are separated by 374 nucleotides BWI76_RS26500 and BWI76_RS26505 overlap by 1 nucleotides BWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotides
BWI76_RS26495: BWI76_RS26495 - LysR family transcriptional regulator, at 5,483,740 to 5,484,600
_RS26495
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487
_RS26505
BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317
_RS26510
Position (kb)
5484
5485
5486
5487 Strain fitness (log2 ratio)
-1
0
1
2
3 at 5483.993 kb on + strand, within BWI76_RS26495 at 5484.001 kb on - strand, within BWI76_RS26495 at 5484.083 kb on + strand, within BWI76_RS26495 at 5484.417 kb on + strand, within BWI76_RS26495 at 5484.585 kb on + strand at 5484.585 kb on + strand at 5484.616 kb on - strand at 5484.616 kb on - strand at 5484.716 kb on + strand at 5485.097 kb on + strand at 5485.151 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.313 kb on - strand, within BWI76_RS26500 at 5485.480 kb on - strand, within BWI76_RS26500 at 5485.638 kb on - strand, within BWI76_RS26500 at 5485.643 kb on - strand, within BWI76_RS26500 at 5485.711 kb on - strand, within BWI76_RS26500 at 5485.796 kb on - strand, within BWI76_RS26500 at 5485.836 kb on + strand, within BWI76_RS26500 at 5485.839 kb on - strand, within BWI76_RS26500 at 5485.886 kb on - strand, within BWI76_RS26500 at 5486.002 kb on + strand, within BWI76_RS26500 at 5486.303 kb on + strand, within BWI76_RS26500 at 5486.464 kb on + strand at 5486.472 kb on - strand at 5486.493 kb on - strand at 5486.522 kb on + strand at 5486.583 kb on - strand at 5487.167 kb on + strand, within BWI76_RS26505 at 5487.213 kb on + strand, within BWI76_RS26505 at 5487.226 kb on - strand, within BWI76_RS26505 at 5487.305 kb on + strand, within BWI76_RS26505 at 5487.386 kb on - strand, within BWI76_RS26505 at 5487.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,483,993 + BWI76_RS26495 0.29 -1.3 5,484,001 - BWI76_RS26495 0.30 +1.4 5,484,083 + BWI76_RS26495 0.40 +0.7 5,484,417 + BWI76_RS26495 0.79 -1.3 5,484,585 + +0.0 5,484,585 + +0.7 5,484,616 - -0.4 5,484,616 - +0.5 5,484,716 + +1.1 5,485,097 + +0.1 5,485,151 + BWI76_RS26500 0.11 -0.7 5,485,225 + BWI76_RS26500 0.16 +0.4 5,485,225 + BWI76_RS26500 0.16 +1.4 5,485,225 + BWI76_RS26500 0.16 +0.1 5,485,313 - BWI76_RS26500 0.22 -0.2 5,485,480 - BWI76_RS26500 0.32 -1.7 5,485,638 - BWI76_RS26500 0.42 -0.1 5,485,643 - BWI76_RS26500 0.43 -0.2 5,485,711 - BWI76_RS26500 0.47 -0.9 5,485,796 - BWI76_RS26500 0.52 +0.7 5,485,836 + BWI76_RS26500 0.55 -0.9 5,485,839 - BWI76_RS26500 0.55 +0.5 5,485,886 - BWI76_RS26500 0.58 +0.3 5,486,002 + BWI76_RS26500 0.65 -0.5 5,486,303 + BWI76_RS26500 0.85 -0.0 5,486,464 + +0.8 5,486,472 - +0.6 5,486,493 - +1.7 5,486,522 + +0.3 5,486,583 - -0.8 5,487,167 + BWI76_RS26505 0.66 +0.3 5,487,213 + BWI76_RS26505 0.71 +3.7 5,487,226 - BWI76_RS26505 0.72 -1.2 5,487,305 + BWI76_RS26505 0.81 -1.5 5,487,386 - BWI76_RS26505 0.89 +0.1 5,487,448 + +2.4
Or see this region's nucleotide sequence