Experiment: Parafilmed volatile agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25235 and BWI76_RS25240 are separated by 14 nucleotides BWI76_RS25240 and BWI76_RS25245 are separated by 14 nucleotides BWI76_RS25245 and BWI76_RS25250 overlap by 4 nucleotides
BWI76_RS25235: BWI76_RS25235 - calcium/sodium antiporter, at 5,247,422 to 5,248,399
_RS25235
BWI76_RS25240: BWI76_RS25240 - D-arabinose 5-phosphate isomerase, at 5,248,414 to 5,249,400
_RS25240
BWI76_RS25245: BWI76_RS25245 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 5,249,415 to 5,249,981
_RS25245
BWI76_RS25250: BWI76_RS25250 - LPS export ABC transporter periplasmic protein LptC, at 5,249,978 to 5,250,553
_RS25250
Position (kb) 5248
5249
5250 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5247.432 kb on - strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.487 kb on + strand at 5247.553 kb on + strand, within BWI76_RS25235 at 5247.605 kb on - strand, within BWI76_RS25235 at 5247.633 kb on + strand, within BWI76_RS25235 at 5247.699 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand, within BWI76_RS25235 at 5247.883 kb on - strand at 5247.998 kb on + strand, within BWI76_RS25235 at 5248.166 kb on + strand, within BWI76_RS25235 at 5248.283 kb on + strand, within BWI76_RS25235 at 5248.369 kb on + strand at 5248.369 kb on + strand at 5248.377 kb on - strand at 5248.409 kb on + strand at 5248.489 kb on + strand at 5248.489 kb on + strand at 5248.694 kb on - strand, within BWI76_RS25240 at 5248.730 kb on - strand, within BWI76_RS25240 at 5248.858 kb on - strand, within BWI76_RS25240 at 5248.882 kb on + strand, within BWI76_RS25240 at 5248.907 kb on - strand, within BWI76_RS25240 at 5248.917 kb on + strand, within BWI76_RS25240 at 5249.215 kb on - strand, within BWI76_RS25240 at 5249.395 kb on + strand at 5249.446 kb on + strand at 5249.595 kb on + strand, within BWI76_RS25245 at 5249.720 kb on + strand, within BWI76_RS25245 at 5249.823 kb on + strand, within BWI76_RS25245 at 5249.945 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus remove 5,247,432 - -1.0 5,247,487 + +1.3 5,247,487 + +1.7 5,247,487 + +0.0 5,247,487 + -1.4 5,247,553 + BWI76_RS25235 0.13 -0.1 5,247,605 - BWI76_RS25235 0.19 -0.6 5,247,633 + BWI76_RS25235 0.22 +1.8 5,247,699 - BWI76_RS25235 0.28 +0.2 5,247,883 - BWI76_RS25235 0.47 -0.1 5,247,883 - +1.7 5,247,998 + BWI76_RS25235 0.59 +0.6 5,248,166 + BWI76_RS25235 0.76 -0.1 5,248,283 + BWI76_RS25235 0.88 +0.5 5,248,369 + +0.3 5,248,369 + -0.6 5,248,377 - +0.4 5,248,409 + -0.6 5,248,489 + +0.1 5,248,489 + +0.4 5,248,694 - BWI76_RS25240 0.28 -0.2 5,248,730 - BWI76_RS25240 0.32 -0.8 5,248,858 - BWI76_RS25240 0.45 -0.1 5,248,882 + BWI76_RS25240 0.47 -0.1 5,248,907 - BWI76_RS25240 0.50 +0.3 5,248,917 + BWI76_RS25240 0.51 -0.0 5,249,215 - BWI76_RS25240 0.81 +0.4 5,249,395 + +0.1 5,249,446 + -0.1 5,249,595 + BWI76_RS25245 0.32 -2.0 5,249,720 + BWI76_RS25245 0.54 -0.9 5,249,823 + BWI76_RS25245 0.72 -1.3 5,249,945 + +0.1
Or see this region's nucleotide sequence