Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23140

Experiment: Parafilmed volatile agar plate with no fungus

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23130 and BWI76_RS23135 overlap by 11 nucleotidesBWI76_RS23135 and BWI76_RS23140 are separated by 19 nucleotidesBWI76_RS23140 and BWI76_RS23145 are separated by 24 nucleotidesBWI76_RS23145 and BWI76_RS23150 overlap by 1 nucleotides BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429 _RS23130 BWI76_RS23135: BWI76_RS23135 - propanediol dehydratase, at 4,823,419 to 4,823,769 _RS23135 BWI76_RS23140: BWI76_RS23140 - carboxysome shell protein, at 4,823,789 to 4,824,064 _RS23140 BWI76_RS23145: BWI76_RS23145 - propanediol utilization protein, at 4,824,089 to 4,824,550 _RS23145 BWI76_RS23150: BWI76_RS23150 - propanediol utilization phosphotransacylase, at 4,824,550 to 4,825,182 _RS23150 Position (kb) 4823 4824 4825Strain fitness (log2 ratio) -1 0 1 2at 4822.955 kb on - strand, within BWI76_RS23130at 4823.457 kb on - strand, within BWI76_RS23135at 4823.491 kb on + strand, within BWI76_RS23135at 4823.559 kb on + strand, within BWI76_RS23135at 4823.567 kb on - strand, within BWI76_RS23135at 4823.914 kb on - strand, within BWI76_RS23140at 4823.914 kb on - strand, within BWI76_RS23140at 4824.082 kb on + strandat 4824.675 kb on - strand, within BWI76_RS23150at 4824.841 kb on + strand, within BWI76_RS23150at 4824.879 kb on + strand, within BWI76_RS23150

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Per-strain Table

Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with no fungus
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4,822,955 - BWI76_RS23130 0.74 +0.3
4,823,457 - BWI76_RS23135 0.11 -0.7
4,823,491 + BWI76_RS23135 0.21 -0.6
4,823,559 + BWI76_RS23135 0.40 -0.2
4,823,567 - BWI76_RS23135 0.42 +1.8
4,823,914 - BWI76_RS23140 0.45 -0.8
4,823,914 - BWI76_RS23140 0.45 -1.1
4,824,082 + +0.3
4,824,675 - BWI76_RS23150 0.20 +0.3
4,824,841 + BWI76_RS23150 0.46 +0.5
4,824,879 + BWI76_RS23150 0.52 -0.8

Or see this region's nucleotide sequence